Faculty

Tza-Huei (Jeff) Wang

Professor

Research Interests

BioMEMS and microfluidics, single molecule manipulation and detection, nano/micro scale fabrication, conformational dynamics of biomolecules.

Education
  • Ph.D. 2002, University of California, Los Angeles
  • M.S. 1994, National Taiwan University
  • B.S. 1992, National Taiwan University
Research Areas
  • BioMEMS and Microfluidics
  • Nano/Micro Scale Fabrication
  • Single Molecule Manipulation and Detection
  • Genetic and Epigenetic Biomarker for cancer
Awards
  • 2016:  Best Paper Award
  • 2016:  Fellow of American Institute for Medical and Biological Engineering (AIMBE)
  • 2016:  Best Paper Award, The 29th IEEE International Conference on Micro Electro Mechanical Systems (MEMS 2016)
  • 2015:  Siebel Scholar Award awarded to Wang's student Dong Jin Shin
  • 2015:  Johns Hopkins Discovery Award
  • 2014:  JHU PURA awarded to Wang’s student Polly Ma
  • 2014:  Viterbi Postdoctoral Fellowship awarded to Wang’s student Tushar Rane
  • 2013:  Burroughs Wellcome Fund Career Award at the Scientific Interface awarded to Wang's postdoc Stephanie Fraley
  • 2013:  JALA & JBS Art of Science Award awarded to Wang's students Y Zhang and C Beh
  • 2013:  Siebel Scholar Award awarded to Wang's student Tushar Rane
  • 2012:  Best Paper Award - The 6th IEEE International Conference on Nano/Molecular Medicine and Engineering (IEEE NANOMED 2012)
  • 2012:  Burroughs Wellcome Fund Career Award at the Scientific Interface
  • 2012:  JALA & JBS Art of Science Award awarded to Wang's students Y Zhang and C Beh
  • 2012:  NIST/ Lab on a Chip Art in Science Award - micro-TAS 2012 - awarded to Wang's graduate student Yi Zhang
  • 2012:  Siebel Scholar Award awarded to Wang's student Yi Zhang
  • 2011:  Best Paper Award - The 6th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (IEEE NEMS 2011)
  • 2011:  Best Poster Award - 5th Annual INBT NanoBio Symposium
  • 2011:  Early Career Award from Thrasher Research Fund awarded to Wang's postdoc Seungkung Park
  • 2011:  JALA Ten Award - Journal of Laboratory Automation - Society for Laboratory Automation and Screening
  • 2011:  NIST/ Lab on a Chip Art in Science Award - micro-TAS 2011 - awarded to Wang's graduate student DJ Shin and Yi Zhang
  • 2010:  American Association for Cancer Research (AACR) Scholar-in-Training Award awarded to Wang's graduate student Yi Zhang
  • 2010:  Best Poster Award - 4th Johns Hopkins NanoBio Symposium
  • 2010:  Siebel Scholar Award awarded to Wang's graduate student Kelvin Liu
  • 2009:  Best Poster Award - 3rd Johns Hopkins NanoBio Symposium
  • 2009:  Johns Hoplins University WSE Dean’s Leadership Award
  • 2009:  Siebel Scholar Award awarded to Wang's graduate student Vas Bailey
  • 2007:  Co-author and Faculty Advisor to Hunter Chen - winner of Excellence in Research Award - American Society of Gene Therapy
  • 2007:  CRS Jorge Heller Award - Journal of Controlled Release Oustanding Paper Award
  • 2006:  CAREER Award - National Science Foundatiom
Journal Articles
  • Rao, L., Meng, Q. F., Bu, L. L., Cai, B., Huang, Q., Sun, Z. J., Zhang, W. F., Li , A., Guo, S. S., Liu, W., Wang, T. H., Zhao, X. Z. (2017).  Erythrocyte Membrane-Coated Upconversion Nanoparticles with Minimal Protein Adsorption for Enhanced Tumor Imaging..  ACS applied materials & interfaces.  9(3).  2159-2168.
  • Kilburn, D., Song, Y., Wang, T. H., Liu, K. J. (2017).  A Multiplex Ligation Assay for miRNA Copy Number Profiling..  Methods in molecular biology (Clifton, N.J.).  1509.  185-193.
  • Zhang, Y., Zhang, Y., Burke, J. M., Gleitsman, K., Friedrich, S. M., Liu, K. J., Wang, T. H. (2016).  A Simple Thermoplastic Substrate Containing Hierarchical Silica Lamellae for High-Molecular-Weight DNA Extraction..  Advanced materials (Deerfield Beach, Fla.).  28(48).  10630-10636.
  • Santos, J. L., Ren, Y., Vandermark, J., Archang, M. M., Williford, J. M., Liu, H. W., Lee, J., Wang, T. H., Mao, H. (2016).  Continuous Production of Discrete Plasmid DNA-Polycation Nanoparticles Using Flash Nanocomplexation..  Small (Weinheim an der Bergstrasse, Germany).  12(45).  6214-6222.
  • Hulbert, A., Jusue-Torres, I., Stark, A., Chen, C., Rodgers, K., Lee, B., Griffin, C., Yang, A., Huang, P., Wrangle, J., Belinsky, S. A., Wang, T. H., Yang, S. C., Baylin, S. B., Brock, M. V., Herman, J. G. (2016).  Early Detection of Lung Cancer Using DNA Promoter Hypermethylation in Plasma and Sputum..  Clinical cancer research : an official journal of the American Association for Cancer Research.
  • Pisanic, 2nd, T. R., Athamanolap, P., Wang, T. H. (2016).  Defining, distinguishing and detecting the contribution of heterogeneous methylation to cancer heterogeneity..  Seminars in cell & developmental biology.
  • Ford Carleton, P., Schachter, S., Parrish, J. A., Collins, J. M., Crocker, J. B., Dixon, R. F., Edgman-Levitan, S., Lewandrowski, K. B., Stahl, J. E., Klapperich, C., Cabodi, M., Gaydos, C. A., Rompalo, A. M., Manabe, Y., Wang, T. H., Rothman, R., Geddes, C. D., Widdice, L., Jackman, J., Mathura, R. A., Lash, T. B. (2016).  National Institute of Biomedical Imaging and Bioengineering Point-of-Care Technology Research Network: Advancing Precision Medicine..  IEEE journal of translational engineering in health and medicine.  4.  2800614.
  • Lao, Y. H., Chi, C. W., Friedrich, S. M., Peck, K., Wang, T. H., Leong, K. W., Chen, L. C. (2016).  Signal-on Protein Detection via Dye Translocation between Aptamer and Quantum Dot..  ACS applied materials & interfaces.  8(19).  12048-55.
  • Rao, L., Bu, L. L., Cai, B., Xu, J. H., Li , A., Zhang, W. F., Sun, Z. J., Guo, S. S., Liu, W., Wang, T. H., Zhao, X. Z. (2016).  Cancer Cell Membrane-Coated Upconversion Nanoprobes for Highly Specific Tumor Imaging..  Advanced materials (Deerfield Beach, Fla.).  28(18).  3460-6.
  • Friedrich, S. M., Zec, H. C., Wang, T. H. (2016).  Analysis of single nucleic acid molecules in micro- and nano-fluidics..  Lab on a chip.  16(5).  790-811.
  • Stark, A., Shin, D. J., Pisanic, 2nd, T., Hsieh, K., Wang, T. H. (2016).  A parallelized microfluidic DNA bisulfite conversion module for streamlined methylation analysis..  Biomedical microdevices.  18(1).  5.
  • Fraley, S. I., Athamanolap, P., Masek, B. J., Hardick, J., Carroll, K. C., Hsieh, Y. H., Rothman, R. E., Gaydos, C. A., Wang, T. H., Yang, S. (2016).  Nested Machine Learning Facilitates Increased Sequence Content for Large-Scale Automated High Resolution Melt Genotyping..  Scientific reports.  6.  19218.
  • Friedrich, S. M., Liu, K. J., Wang, T. H. (2016).  Single Molecule Hydrodynamic Separation Allows Sensitive and Quantitative Analysis of DNA Conformation and Binding Interactions in Free Solution..  Journal of the American Chemical Society.  138(1).  319-27.
  • Rane, T. D., Zec, H. C., Wang, T. H. (2015).  A Barcode-Free Combinatorial Screening Platform for Matrix Metalloproteinase Screening.  Anal Chem.
  • Hsieh, K., Zec, H. C., Ma, P. C., Rane, T. D., Wang, J. (2015).  Enhancing Throughput of Combinatorial Droplet Devices via Droplet Bifurcation, Parallelized Droplet Fusion, and Parallelized Detection.  Micromachines.  6(10).  1490-1504.
  • Zhang, Y., Wang, J. (2015).  High-resolution quantification by charge-dominant electrophoretic mobility shift of quantum dots.  Electrophoresis.  36(7-8).  1011-1015.
  • Rane, T. D., Chen, L., Zec, H. C., Wang, J. (2015).  Microfluidic continuous flow digital loop-mediated isothermal amplification (LAMP).  Lab on a Chip.  15(3).  776-782.
  • Pisanic, 2nd, T. R., Athamanolap, P., Poh, W., Chen, C., Hulbert, A., Brock, M. V., Herman, J. G., Wang, T. H. (2015).  DREAMing: a simple and ultrasensitive method for assessing intratumor epigenetic heterogeneity directly from liquid biopsies..  Nucleic acids research.  43(22).  e154.
  • Rao, L., Bu, L. L., Xu, J. H., Cai, B., Yu, G. T., Yu, X., He, Z., Huang, Q., Li , A., Guo, S. S., Zhang, W. F., Liu, W., Sun, Z. J., Wang, H., Wang, T. H., Zhao, X. Z. (2015).  Red Blood Cell Membrane as a Biomimetic Nanocoating for Prolonged Circulation Time and Reduced Accelerated Blood Clearance..  Small (Weinheim an der Bergstrasse, Germany).  11(46).  6225-36.
  • Song, Y., Liu, K. J., Wang, T. H. (2015).  Efficient synthesis of stably adenylated DNA and RNA adapters for microRNA capture using T4 RNA ligase 1..  Scientific reports.  5.  15620.
  • Guan, W., Chen, L., Rane, T. D., Wang, T. H. (2015).  Droplet Digital Enzyme-Linked Oligonucleotide Hybridization Assay for Absolute RNA Quantification..  Scientific reports.  5.  13795.
  • Shin, D. J., Zhang, Y., Wang, T. (2014).  A droplet microfluidic approach to single-stream nucleic acid isolation and mutation detection.  Microfluidics and Nanofluidics.  17(2).  425-430.
  • Beh, C. W., Pan, D., Lee, J., Jiang, X., Liu, K. J., Mao, H., Wang, T. (2014).  Direct Interrogation of DNA Content Distribution in Nanoparticles by a Novel Microfluidics-Based Single-Particle Analysis.  Nano Letters.  14(8).  4729-4735.
  • Athamanolap, P., Shin, D. J., Wang, T. (2014).  Droplet Array Platform for High-Resolution Melt Analysis of DNA Methylation Density.  Jala.  19(3).  304-312.
  • Song, Y., Liu, K. J., Wang, T. (2014).  Elimination of Ligation Dependent Artifacts in T4 RNA Ligase to Achieve High Efficiency and Low Bias MicroRNA Capture.  Plos One.  9(4).
  • Zhang, Y., Wang, T. H. (2014).  High-resolution quantification by charge-dominant electrophoretic mobility shift of quantum dots.  Electrophoresis.
  • Zhang, L., Song, Y., Fujita, T., Zhang, Y., Chen, M., Wang, T. (2014).  Large Enhancement of Quantum Dot Fluorescence by Highly Scalable Nanoporous Gold.  Advanced Materials.  26(8).  1289-1294.
  • Shin, D. J., Wang, T. (2014).  Magnetic Droplet Manipulation Platforms for Nucleic Acid Detection at the Point of Care.  Annals of Biomedical Engineering.  42(11).  2289-2302.
  • Rane, T. D., Chen, L., Zec, H. C., Wang, T. H. (2014).  Microfluidic continuous flow digital loop-mediated isothermal amplification (LAMP).  Lab Chip.
  • Zec, H., Shin, D. J., Wang, T. (2014).  Novel droplet platforms for the detection of disease biomarkers.  Expert Review of Molecular Diagnostics.  14(7).  787-801.
  • Nalayanda, D. D., Fulton, W. B., Colombani, P. M., Wang, T., Abdullah, F. (2014).  Pressure induced lung injury in a novel in vitro model of the alveolar interface: Protective effect of dexamethasone.  Journal of Pediatric Surgery.  49(1).  61-65.
  • Pisanic, II, T. R., Zhang, Y., Wang, T. H. (2014).  Quantum dots in diagnostics and detection: principles and paradigms.  Analyst.  139(12).  2968-2981.
  • Guzzetta, A. A., Pisanic, Ii, T. R., Sharma, P., Yi, J. M., Stark, A., Wang, T. H., Ahuja, N. (2014).  The promise of methylation on beads for cancer detection and treatment.  Expert Rev Mol Diagn.  14(7).  845-52.
  • Athamanolap, P., Parekh, V., Fraley, S. I., Agarwal, V., Shin, D. J., Jacobs, M., Wang, T., Yang, S. (2014).  Trainable High Resolution Melt Curve Machine Learning Classifier for Large-Scale Reliable Genotyping of Sequence Variants.  Plos One.  9(10).
  • Nalayanda, D. D., Fulton, W. B., Wang, T., Abdullah, F. (2013).  A multiphase fluidic platform for studying ventilator-induced injury of the pulmonary epithelial barrier.  Integrative Biology.  5(9).  1141-1148.
  • Zhang, Y., Park, S., Yang, S., Wang, T. (2013).  An all-in-one microfluidic device for parallel DNA extraction and gene analysis (vol 12, pg 1043, 2010).  Biomedical Microdevices.  15(2).  383.
  • Wang, T. H. (2013).  Discerning single molecule interactions of DNA and quantum dots.  Biotechnology Journal.  8(1).  15-16.
  • Keeley, B., Stark, A., Pisanic, II, T. R., Kwak, R., Zhang, Y., Wrangle, J., Baylin, S., Herman, J., Ahuja, N., Brock, M. V., Wang, T. (2013).  Extraction and processing of circulating DNA from large sample volumes using methylation on beads for the detection of rare epigenetic events.  Clinica Chimica Acta.  425.  169-175.
  • Zhang, Y., Wang, T. H. (2013).  Full-range magnetic manipulation of droplets via surface energy traps enables complex bioassays.  Adv Mater.  25(21).  2903-8.
  • Wang, T. (2013).  Micro and Nanotechnologies Enhanced Biomolecular Sensing.  Biosensors.  3(3).  283-285.
  • Yi, J. M., Guzzetta, A. A., Bailey, V. J., Downing, S. R., Van Neste, L., Chiappinelli, K. B., Keeley, B. P., Stark, A., Herrera, A., Wolfgang, C., Pappou, E. P., Iacobuzio-Donahue, C. A., Goggins, M. G., Herman, J. G., Wang, T., Baylin, S. B., Ahuja, N. (2013).  Novel Methylation Biomarker Panel for the Early Detection of Pancreatic Cancer.  Clinical Cancer Research.  19(23).  6544-6555.
  • Zhang, Y., Shin, D. J., Wang, T. (2013).  Serial dilution via surface energy trap-assisted magnetic droplet manipulation.  Lab on a Chip.  13(24).  4827-4831.
  • Song, Y., Zhang, Y., Wang, T. (2013).  Single Quantum Dot Analysis Enables Multiplexed Point Mutation Detection by Gap Ligase Chain Reaction.  Small.  9(7).  1096-1105.
  • Chiou, C., Shin, D. J., Zhang, Y., Wang, T. (2013).  Topography-assisted electromagnetic platform for blood-to-PCR in a droplet.  Biosensors & Bioelectronics.  50.  91-99.
  • Fraley, S. I., Hardick, J., Masek, B. J., Athamanolap, P., Rothman, R. E., Gaydos, C. A., Carroll, K. C., Wakefield, T., Wang, T., Yang, S. (2013).  Universal digital high-resolution melt: a novel approach to broad-based profiling of heterogeneous biological samples.  Nucleic Acids Research.  41(18).
  • Rane, T. D., Zec, H. C., Wang, T. H. (2012).  A serial sample loading system: interfacing multiwell plates with microfluidic devices.  J Lab Autom.  17(5).  370-7.
  • Rane, T. D., Zec, H. C., Puleo, C., Lee, A. P., Wang, T. H. (2012).  Droplet microfluidics for amplification-free genetic detection of single cells.  Lab on a Chip.  12(18).  3341-7.
  • Zhang, Y., Liu, K. J., Wang, T. L., Shih, I., Wang, T. H. (2012).  Mapping DNA Quantity into Electrophoretic Mobility through Quantum Dot Nanotethers for High-Resolution Genetic and Epigenetic Analysis..  ACS Nano.  6(1).  858-64.
  • Zhang, Y., Wang, T. H. (2012).  Micro magnetic gyromixer for speeding up reactions in droplets.  Microfluidics and Nanofluidics.  12(5).  787-794.
  • Zec, H., Rane, T. D., Wang, T. H. (2012).  Microfluidic platform for on-demand generation of spatially indexed combinatorial droplets.  Lab on a Chip.  12(17).  3055-3062.
  • Beh, C. W., Zhou, W. Z., Wang, T. H. (2012).  PDMS-glass bonding using grafted polymeric adhesive - alternative process flow for compatibility with patterned biological molecules.  Lab on a Chip.  12(20).  4120-4127.
  • Zhang, Y., Wang, T. H. (2012).  Quantum dot enabled molecular sensing and diagnostics.  Theranostics.  2(7).  631-54.
  • Song, Y., Zhang, Y., Wang, T. H. (2012).  Single Quantum Dot Analysis Enables Multiplexed Point Mutation Detection by Gap Ligase Chain Reaction.  Small.
  • Zhang, Y., Park, S., Liu, K., Tsuan, J., Yang, S., Wang, T. (2011).  A surface topography assisted droplet manipulation platform for biomarker detection and pathogen identification.  Lab on a Chip.  11(3).  398-406.
  • Park, S., Zhang, Y., Lin, S., Wang, T. H., Yang, S. (2011).  Advances in microfluidic PCR for point-of-care infectious disease diagnostics.  Biotechnology Advances.  29(6).  830-839.
  • Park, S., Zhang, Y., Wang, T. H., Yang, S. (2011).  Continuous dielectrophoretic bacterial separation and concentration from physiological media of high conductivity.  Lab on a Chip - Miniaturisation for Chemistry and Biology.  11(17).  2893-2900.
  • Zhang, Y., Wang, T. H. (2011).  Quantum dots-enabled high-resolution analysis of gene copy number variation: Electrophoretic migration and band broadening of QD-DNA nanocomplex.  IEEE Nanotechnology Magazine.  5(2).  23-27.
  • Liu, K. J., Rane, T. D., Zhang, Y., Wang, T. H. (2011).  Single-molecule analysis enables free solution hydrodynamic separation using yoctomole levels of DNA.  Journal of the American Chemical Society.  133(18).  6898-6901.
  • Zhang, Y., Park, S., Yang, S., Wang, T. (2010).  An all-in-one microfluidic device for parallel DNA extraction and gene analysis.  Biomedical Microdevices.  12(6).  1043-1049.
  • Zhang, Y., Park, S., Yang, S., Wang, T. H. (2010).  An all-in-one microfluidic device for parallel DNA extraction and gene analysis..  Biomed Microdevices.  12(6).  1043-9.
  • RANE, T., PULEO, C., LIU, K., ZHANG, Y., LEE, A., WANG, T. (2010).  Counting single molecules in sub-nanolitre droplets.  Lab on a Chip.  10(2).  161-164.
  • Liu, K. J., Brock, M. V., Shih, I. M., Wang, T. H. (2010).  Decoding circulating nucleic acids in human serum using microfluidic single molecule spectroscopy.  Journal of the American Chemical Society.  132(16).  5793-5798.
  • Bailey, V. J., Keeley, B. P., Razavi, C. R., Griffiths, E., Carraway, H. E., Wang, T. (2010).  DNA methylation detection using MS-qFRET, a quantum dot-based nanoassay.  Methods.  52(3).  237-241.
  • Bailey, V. J., Keeley, B. P., Razavi, C. R., Griffiths, E., Carraway, H. E., Wang, T. H. (2010).  DNA methylation detection using MS-qFRET, a quantum dot-based nanoassay..  Methods.  52(3).  237-41.
  • Jiang, X., Zheng, Y., Chen, H. H., Leong, K. W., Wang, T. H., Mao, H. (2010).  Dual-sensitive micellar nanoparticles regulate dna unpacking and enhance gene-delivery efficiency.  Advanced Materials.  22(23).  2556-2560.
  • Nalayanda, D. D., Wang, Q., Fulton, W. B., Wang, T. H., Abdullah, F. (2010).  Engineering an artificial alveolar-capillary membrane: a novel continuously perfused model within microchannels.  Journal of Pediatric Surgery.  45(1).  45-51.
  • Bailey, V. J., Keeley, B. P., Zhang, Y., Ho, Y. P., Easwaran, H., Brock, M. V., Pelosky, K. L., Carraway, H. E., Baylin, S. B., Herman, J. G., Wang, T. H. (2010).  Enzymatic incorporation of multiple dyes for increased sensitivity in QD-FRET sensing for DNA methylation detection.  ChemBioChem.  11(1).  71-74.
  • Wang, T., Bailey, V., Zhang, Y., Liu, K. (2010).  Quantum Dots DNA Nanosensors - Ultrasensitive Platform for Detecting Genomic Cancer Markers.  BIOforum Europe.  1-2.  28-30.
  • Bailey, V. J., Zhang, Y., Keeley, B. P., Yin, C., Pelosky, K. L., Brock, M., Baylin, S. B., Herman, J. G., Wang, T. H. (2010).  Single-tube analysis of DNA methylation with silica superparamagnetic beads.  Clinical Chemistry.  56(6).  1022-1025.
  • Wang, T., Wong, P. K. (2010).  Transforming Microfluidics into Laboratory Automation.  Jala.  15(3).  A15-A16.
  • Wang, T. H., Wong, P. K. (2010).  Transforming Microfluidics into Laboratory Automation.  JALA - Journal of the Association for Laboratory Automation.  15(3).  A15-A16.
  • Schiminovich, D., Catinella, B., Kauffmann, G., Fabello, S., Wang, J., Hummels, C., Lemonias, J., Moran, S., Wu, R., Giovanelli, R., Haynes, M., Heckman, T., Basu-Zych, A., Blanton, M., Brinchmann, J., Budavari, T., Goncalves, T., Johnson, B., Kennicutt, R., Madore, B., Martin, C., Rich, M., Tacconi, L., Thilker, D., Wild, V., Wyder, T. (2010).  The GALEX Arecibo SDSS Survey - II. The star formation efficiency of massive galaxies.  Monthly Notices of the Royal Astronomical Society.  408.  919-934.
  • Catinella, B., Schiminovich, D., Kauffmann, G., Fabello, S., Wang, J., Hummels, C., Lemonias, J., Moran, S., Wu, R., Giovanelli, R., Haynes, M., Heckman, T., Basu-Zych, A., Blanton, M., Brinchmann, J., Budavari, T., Goncalves, T., Johnson, B., Kennicutt, R., Madore, B., Martin, C., Rich, M., Tacconi, L., Thilker, D., Wild, V., Wyder, T. (2010).  The GALEX Arecibo SDSS Survey - I. Gas fraction scaling relations of massive galaxies and first data release.  Monthly Notices of the Royal Astronomical Society.  403.  683-708.
  • NALAYANDA, D., PULEO, C., FULTON, W., SHARPE, L., WANG, T., ABDULLAH, F. (2009).  An open-access microfluidic model for lung-specific functional studies at an air-liquid interface.  Biomedical Microdevices.  11(5).  1081-1089.
  • Ho, Y. P., Chen, H. H., Leong, K. W., Wang, T. H. (2009).  Combining QD-FRET and microfluidics to monitor DNA nanocomplex self-assembly in real-time.  Journal of visualized experiments : JoVE.  (30).
  • Zhang, Y., Bailey, V., Puleo, C., Easwaran, H., Griffiths, E., Herman, J., Baylin, S., Wang, T. (2009).  DNA methylation analysis on a droplet-in-oil PCR array.  Lab on a Chip.  1059-1064.
  • Chen, H. H., Ho, Y. P., Jiang, X., Mao, H., Wang, T. H., Leong, K. W. (2009).  Erratum to "Simultaneous non-invasive analysis of DNA condensation and stability by two-step QD-FRET" [Nano Today 4 (2009) 125-134] (DOI:10.1016/j.nantod.2009.02.008).  Nano Today.  4(3).  280.
  • PULEO, C., AMBROSE, W., TAKEZAWA, T., ELISSEEFF, J., WANG, T. (2009).  Integration and application of vitrified collagen in multilayered microfluidic devices for corneal microtissue culture.  Lab on a Chip.  9(22).  3221-3227.
  • Puleo, C. M., Wang, T. H. (2009).  Microfluidic means of achieving attomolar detection limits with molecular beacon probes.  Lab on a Chip - Miniaturisation for Chemistry and Biology.  9(8).  1065-1072.
  • Puleo, C. M., Wang, T. (2009).  Microfluidic means of achieving attomolar detection limits with molecular beacon probes.  Lab on a Chip.  9(8).  1065-1072.
  • BAILEY, V., EASWARAN, H., ZHANG, Y., GRIFFITHS, E., BELINSKY, S., HERMAN, J., BAYLIN, S., CARRAWAY, H., WANG, T. (2009).  MS-qFRET: A quantum dot-based method for analysis of DNA methylation.  Genome Research.  19(8).  1455-1461.
  • CHEN, H., HO, Y., JIANG, X., MAO, H., WANG, T., LEONG, K. (2009).  Simultaneous non-invasive analysis of DNA condensation and stability by two-step QD-FRET.  Nano Today.  4(2).  125-134.
  • Ho, Y. P., Chen, H. H., Leong, K. W., Wang, T. H. (2009).  The convergence of quantum-dot-mediated fluorescence resonance energy transfer and microfluidics for monitoring DNA polyplex self-assembly in real time.  Nanotechnology.  20(9).
  • Puleo, C. M., Yeh, H. C., Liu, K. J., Wang, T. H. (2008).  Coupling confocal fluorescence detection and recirculating microfluidic control for single particle analysis in discrete nanoliter volumes.  Lab on a Chip.  8(5).  822-825.
  • Liu, K. J., Wang, T. H. (2008).  Cylindrical illumination confocal spectroscopy: Rectifying the limitations of confocal single molecule spectroscopy through one-dimensional beam shaping.  Biophysical Journal.  95(6).  2964-2975.
  • Liu, K. J., Zhang, Y., Wang, T. H. (2008).  Detect the dots.  IEEE Nanotechnology Magazine.  2(1).  14-18.
  • Lim, T. C., Bailey, V. J., Ho, Y. P., Wang, T. H. (2008).  Intercalating dye as an acceptor in quantum-dot-mediated FRET.  Nanotechnology.  19(7).
  • CHEN, H., HO, Y., JIANG, X., MAO, H., WANG, T., LEONG, K. (2008).  Quantitative comparison of intracellular unpacking kinetics of polyplexes by a model constructed from quantum Dot-FRET.  Molecular Therapy.  16(2).  324-332.
  • Sun, T. M., Du, J. Z., Yan, L. F., Mao, H., Wang, J. (2008).  Self-assembled biodegradable micellar nanoparticles of amphiphilic and cationic block copolymer for siRNA delivery.  Biomaterials.  29(32).  4348-4355.
  • Yeh, H. C., Puleo, C. M., Ho, Y. P., Bailey, V. J., Teck, C. L., Liu, K., Wang, T. H. (2008).  Tunable blinking kinetics of Cy5 for precise DNA quantification and single-nucleotide difference detection.  Biophysical Journal.  95(2).  729-737.
  • Puleo, C. M., Yeh, H. C., Wang, T. H. (2007).  Applications of MEMS technologies in tissue engineering.  Tissue Engineering.  13(12).  2839-2854.
  • Nalayanda, D. D., Puleo, C. M., Fulton, W. B., Wang, T. H., Abdullah, F. (2007).  Characterization of pulmonary cell growth parameters in a continuous perfusion microfluidic environment.  Experimental Lung Research.  33(6).  321-335.
  • Nathani, H., Wang, J., Weihs, T. (2007).  Long-term stability of nanostructured systems with negative heats of mixing.  Journal of Applied Physics.  101(10).
  • Chao, S. Y., Ho, Y. P., Bailey, V. J., Wang, T. H. (2007).  Quantification of low concentrations of DNA using single molecule detection and velocity measurement in a microchannel.  Journal of Fluorescence.  17(6).  767-774.
  • Yeh, H. C., Puleo, C. M., Lim, T. C., Ho, Y. P., Giza, P. E., Huang, R., Wang, T. H. (2006).  A microfluidic-FCS platform for investigation on the dissociation of Sp1-DNA complex by doxorubicin.  Nucleic Acids Research.  34(21).
  • HO, Y., CHEN, H., LEONG, K., WANG, T. (2006).  Evaluating the intracellular stability and unpacking of DNA nanocomplexes by quantum dots-FRET.  Journal of Controlled Release.  116(1).  83-89.
  • YEH, H., HO, Y., SHIH, I., WANG, T. (2006).  Homogeneous point mutation detection by quantum dot-mediated two-color fluorescence coincidence analysis.  Nucleic Acids Research.  34(5).
  • Puleo, C. M., Liu, K., Wang, T. (2006).  Pushing miRNA quantification to the limits: high-throughput miRNA gene expression analysis using single-molecule detection.  Nanomedicine.  1(1).  123-127.
  • ZHANG, C., CHAO, S., WANG, T. (2005).  Comparative quantification of nucleic acids using single-molecule detection and molecular beacons.  Analyst.  130(4).  483-488.
  • Wang, J., Besnoin, E., Knio, O. M., Weihs, T. (2005).  Effects of physical properties of components on reactive nanolayer joining.  Journal of Applied Physics.  97(11).
  • Eagleton, T. S., Mallet, J., Cheng, X., Wang, J., Chien, C., Searson, P. (2005).  Electrodeposition of CoxPt1-x thin films.  Journal of the Electrochemical Society.  152(1).  C27-C31.
  • Trenkle, J. C., Wang, J., Weihs, T., Hufnagel, T. (2005).  Microstructural study of an oscillatory formation reaction in nanostructured reactive multilayer foils.  Applied Physics Letters.  87(15).
  • HO, Y., KUNG, M., YANG, S., WANG, T. (2005).  Multiplexed hybridization detection with multicolor colocalization of quantum dot nanoprobes.  Nano Letters.  5(9).  1693-1697.
  • Yeh, H. C., Ho, Y. P., Wang, T. H. (2005).  Quantum dot-mediated biosensing assays for specific nucleic acid detection..  Nanomedicine.  1(2).  115-21.
  • Yeh, H. C., Ho, Y. P., Wang, T. H. (2005).  Quantum dot-mediated biosensing assays for specific nucleic acid detection..  Nanomedicine.  1(2).  115-21.
  • YEH, H., CHAO, S., HO, Y., WANG, T. (2005).  Single-molecule detection and probe strategies for rapid and ultrasensitive genomic detection.  Current Pharmaceutical Biotechnology.  6(6).  453-461.
  • WANG, T., PENG, Y., ZHANG, C., WONG, P., HO, C. (2005).  Single-molecule tracing on a fluidic microchip for quantitative detection of low-abundance nucleic acids.  Journal of the American Chemical Society.  127(15).  5354-5359.
  • ZHANG, C., YEH, H., KUROKI, M., WANG, T. (2005).  Single-quantum-dot-based DNA nanosensor.  Nature Materials.  4(11).  826-831.
  • Zhang, P. C., Wang, J., Leong, K. W., Mao, H. (2005).  Ternary complexes comprising polyphosphoramidate gene carriers with different types of charge groups improve transfection efficiency.  Biomacromolecules.  6(1).  54-60.
  • Li, Y., Wang, J., Lee, C. G., Wang, C. Y., Gao, S. J., Tang, G. P., Ma, Y. X., Yu, H., Mao, H., Leong, K. W., Wang, S. (2004).  CNS gene transfer mediated by a novel controlled release system based on DNA complexes of degradable polycation PPE-EA: A comparison with polyethylenimine/DNA complexes.  Gene Therapy.  11(1).  109-114.
  • WONG, P., CHEN, C., WANG, T., HO, C. (2004).  Electrokinetic bioprocessor for concentrating cells and molecules.  Analytical Chemistry.  76(23).  6908-6914.
  • WONG, P., WANG, T., DEVAL, J., HO, C. (2004).  Electrokinetics in micro devices for biotechnology applications.  IEEE-ASME Transactions on Mechatronics.  9(2).  366-376.
  • Wang, J., Lee, I. L., Lim, W. S., Chia, S. M., Yu, H., Leong, K. W., Mao, H. (2004).  Evaluation of collagen and methylated collagen as gene carriers.  International Journal of Pharmaceutics.  279(1-2).  115-126.
  • Wang, J., Besnoin, E., Knio, O. M., Weihs, T. (2004).  Investigating the effect of applied pressure on reactive multilayer foil joining.  Acta Materialia.  52(18).  5265-5274.
  • Wang, J., Besnoin, E., Duckham, A., Spey, S. J., Reiss, M. E., Knio, O. M., Weihs, T. (2004).  Joining of stainless-steel specimens with nanostructured Al/Ni foils.  Journal of Applied Physics.  95(1).  248-256.
  • Wan, A. C., Mao, H., Wang, S., Phua, S. H., Lee, G. P., Pan, J., Lu, S., Wang, J., Leong, K. W. (2004).  Poly(phosphoester) ionomers as tissue-engineering scaffolds.  Journal of Biomedical Materials Research - Part B Applied Biomaterials.  70(1).  91-102.
  • Wang, J., Gao, S. J., Zhang, P. C., Wang, S., Mao, H., Leong, K. W. (2004).  Polyphosphoramidate gene carriers: Effect of charge group on gene transfer efficiency.  Gene Therapy.  11(12).  1001-1010.
  • Duckham, A., Spey, S. J., Wang, J., Reiss, M. E., Weihs, T., Besnoin, E., Knio, O. M. (2004).  Reactive nanostructured foil used as a heat source for joining titanium.  Journal of Applied Physics.  96(4).  2336-2342.
  • Huang, S. W., Wang, J., Zhang, P. C., Mao, H., Zhuo, R. X., Leong, K. W. (2004).  Water-soluble and nonionic polyphosphoester: Synthesis, degradation, biocompatibility and enhancement of gene expression in mouse muscle.  Biomacromolecules.  5(2).  306-311.
  • Fang, N., Wang, J., Mao, H., Leong, K. W., Chan, V. (2003).  BHEM-Chol/DOPE liposome induced perturbation of phospholipid bilayer.  Colloids and Surfaces B: Biointerfaces.  29(4).  233-245.
  • Wang, J., Huang, S. W., Zhang, P. C., Mao, H., Leong, K. W. (2003).  Effect of side-chain structures on gene transfer efficiency of biodegradable cationic polyphosphoesters.  International Journal of Pharmaceutics.  265(1-2).  75-84.
  • Lu, H. F., Lim, W. S., Wang, J., Tang, Z. Q., Zhang, P. C., Leong, K. W., Chia, S. M., Yu, H., Mao, H. (2003).  Galactosylated PVDF membrane promotes hepatocyte attachment and functional maintenance.  Biomaterials.  24(27).  4893-4903.
  • Zhao, Z., Wang, J., Mao, H., Leong, K. W. (2003).  Polyphosphoesters in drug and gene delivery.  Advanced Drug Delivery Reviews.  55(4).  483-499.
  • Wang, J., Besnoin, E., Duckham, A., Spey, S. J., Reiss, M. E., Knio, O. M., Powers, M., Whitener, M., Weihs, T. (2003).  Room-temperature soldering with nanostructured foils.  Applied Physics Letters.  83(19).  3987-3989.
  • Wang, J., Zhang, P. C., Mao, H., Leong, K. W. (2002).  Enhanced gene expression in mouse muscle by sustained release of plasmid DNA using PPE-EA as a carrier.  Gene Therapy.  9(18).  1254-1261.
  • Wang, J., Zhang, P. C., Lu, H. F., Ma, N., Wang, S., Mao, H., Leong, K. W. (2002).  New polyphosphoramidate with a spermidine side chain as a gene carrier.  Journal of Controlled Release.  83(1).  157-168.
  • Wang, J., Mao, H., Leong, K. W. (2001).  A novel biodegradable gene carrier based on polyphosphoester [19].  Journal of the American Chemical Society.  123(38).  9480-9481.
Book Chapters
  • Wang, T., Liu, K., Yeh, H., Puleo, C. (2010).  Nanobiosensors.  Microtechnology and Nanotechnology in Biomedical Applications.  Oxford University Press.  346-394.
Other Publications
  • Friedrich, S. M., Burke, J. M., Liu, K. J., Wang, T. (2016).  Counter-Flow DNA PreConcentration Without Applied Electric Fields.
  • Zhang, Y., Zhang, Y., Liu, K., Wang, T. (2016).  Heat-Shrunken Hierarchical Silica Nanomembrane for Solid Phase DNA Extraction.
  • Shin, D., Athamanolap, P., Chen, L., Hardick, J., Gaydos, C. A., Wang, T. (2016).  Design and Evaluation of a Mobile Nucleic Acid Amplification Testing (NAAT) System in a Hospital Emergency Setting.
  • Athamanolap, P., Pisanic II, T. R., Wang, T. (2016).  Development of assays for detecting methylation in cell-free DNA at single copy sensitivity and single CpG-site resolution.  2016 Biomedical Engineering Society Meeting (BMES).
  • Friedrich, S., Burke, J., Liu, K., Wang, J. (2016).  In-line Preconcentration, Size Separation, and Single-Molecule Detection Without Applied Electric Fields.  181-184.
  • O’Keefe, C., Pisanic II, P, T., Athamanolop, P., Zec, H., Wang, T. (2016).  Microfluidic Digital Melt Array for Accessing Rare Methylation Biomarkers in Cancer.
  • Liu, L., Hsieh, K., Kaushik, A., Zec, H., Wang, T. (2015).  Multiplexed, Continuous-Flow, Droplet-Based PCR Genotyping Platform for High-Throughput Agriculture Marker Assisted Selection .  1368-1370.
  • Kaushik, A., Heish, K., L., C., Shin, D. J., Wang, T. (2015).  Rapid Assessment of Bacterial Vitality and Antibiotic Susceptibility via High-Throughput Picoliter-Droplet Single-Cell Assay.  531-533.
  • Shin, D. J., Chen, L., Wang, T. (2015).  Single-bacteria Confocal Spectroscopy: An Ultrasensitive method for Real-time Monitoring of Bacterial Growth.  340-342.
  • Shin, D. J., Athamanolap, P., Chen, L., Wang, T. (2014).  Integrated droplet microfluidic platform for nucleic acids amplification test of Chlamydia trachomatis infection.
  • Zec, H., Glove, C., Hsieh, W., Liu, L., Keefe, C., Wang, T. (2014).  Methods for Controlling Water Evaporation in PDMS-Based Microfluidic Devices.
  • Rane, T., Zec, H., Wang, T. (2013).  A Multiplexed Microfluidic Droplet Platform for Matrix Metalloproteinase Screening.  1595-1597.
  • Shin, D., Chen, L., Wang, T. (2013).  A SIMPLE INTEGRATED DIAGNOSTIC PLATFORM FOR DNA TESTING OF CHLAMYDIA TRACHOMATIS INFECTION.  1350-1352.
  • Wu, J., Friedrich, F., Liu, K., Wang, T. (2013).  Chip-Based DNA Separation in Free Solution by Inertial Hydrodynamic Forces.  578-580.
  • Zhang, Y., Zhang, Y., Wang, T. (2013).  Hierarchical Silica Nanomembrane Driven by Thermal Shrinkage and its Application for Solid Phase DNA Extraction.  IEEE.
  • Athamanolap, P., Keeley, B., Shin, D. J., Wang, T. (2013).  Quantitative Analysis of DNA Methylation Based on Melting Curve Analysis.  IEEE.  1116-1118.
  • Friedrich, S., Liu, K., Wang, T. (2013).  SINGLE MOLECULE HYDRODYNAMIC SEPARATION FOR ULTRASENSITIVE AND QUANTITATIVE DNA SIZE SEPARATIONS.  35-37.
  • Zhang, Y., Zhang, Y., Keely, B., Stark, A., Wang, T. (2013).  Spontaneous Shrinking Silica Nanomembrane for Solid Phase.  IEEE.
Conference Proceedings
  • Shin, D., Athamanolap, P., Chen, L., Hardick, J., Gaydos, C. A., Wang, T. (2016).  A Smartphone-Based Mobile NAAT Diagnostic Suite for Chlamydia Detection.  Proc. 26th Anniversary World Congress on Biosensors (Biosensors 2016).
  • Beh, C. W., Zhang, Y., Wang, T. (2016).  Fluorescence Flow Moriometry – Flow Cytometry-Like Analysis Method for Single Molecule and Particle Cauterization.  Proc. 20th International Conference on Miniaturized Chemical and Biochemical Analysis Systems .  1362-1263.
  • Axt, B., Hsieh, Y., Zec, H. C., Hsieh, K., Zheng, Y., Kaushik, A., Wang, T. (2016).  Fully Automated Operation of Microfluidic Device with Impedance based Valve Control.  Proc. 20th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2016).  10003-1004.
  • Friedrich, S. M., Burke, J. M., Liu, K. J., Wang, T. (2016).  In-line Preconcentration, Size Separation, and Single-Molecule Detection Without Applied Electric Fields.  Proc. 29th IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2016).  181-184.
  • Wen, K., Zec, H. C., Chen, L., Kaushik, A., Wang, T. (2016).  Rapid, Accurate, and General Single-Cell Antibiotic Susceptibility Test in Digital Bacterial Picoarray.  Proc. 20th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2016).  174-175.
  • Zheng, T., Zec, H. C., Hsieh, K., Kaushik, A., Axt, B., Hsieh, Y., Wang, T. (2016).  Silicone Oil Improves Molecule Retention for Droplet-Based Bioassays.  Proc. 20th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2016).  1017-1018.
  • Guan, W., Chen, L., Rane, T., Kaushik, A., Wang, J. (2015).  Digital droplet ELOHA for nucleic acid molecule counting and analysis.  Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS), 2015 Transducers-2015 18th International Conference on.  IEEE.  460-463.
  • Liu, L., Hsieh, K., Kaushik, A., Zec, H., Wang, T. (2015).  Multiplexed, Continuous-Flow, Droplet-Based PCR Genotyping Platform for High-Throughput Agriculture Marker Assisted Selection .  19th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2015).  1368-1370.
  • Kaushik, A., Heish, K., L., C., Shin, D. J., Wang, T. (2015).  Rapid Assessment of Bacterial Vitality and Antibiotic Susceptibility via High-Throughput Picoliter-Droplet Single-Cell Assay.  19th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2015).  531-533.
  • Shin, D. J., Chen, L., Wang, T. H. (2015).  Single-bacteria Confocal Spectroscopy: An Ultrasensitive method for Real-time Monitoring of Bacterial Growth.  19th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2015).  340-342.
  • Zec, H., O’Keefe, C., Ma, P., Wang, T. (2015).  Ultra-thin, evaporation-resistent PDMS devices for absolute quantification of DNA using digital PCR.  Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS), 2015 Transducers-2015 18th International Conference on.  IEEE.  536-539.
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