Journal Publications | Patents | Conference Publications

Journal Publications

152 entries « 4 of 8 »
61.

2nd Pisanic, T. R.; Athamanolap, P.; Wang, T. H.

Defining, distinguishing and detecting the contribution of heterogeneous methylation to cancer heterogeneity Journal Article

In: Semin Cell Dev Biol, 64 , pp. 5-17, 2017, ISSN: 1096-3634 (Electronic) 1084-9521 (Linking).

Abstract | Links | BibTeX

62.

Andini, N.; Wang, B.; Athamanolap, P.; Hardick, J.; Masek, B. J.; Thair, S.; Hu, A.; Avornu, G.; Peterson, S.; Cogill, S.; Rothman, R. E.; Carroll, K. C.; Gaydos, C. A.; Wang, J. T.; Batzoglou, S.; Yang, S.

Microbial Typing by Machine Learned DNA Melt Signatures Journal Article

In: Sci Rep, 7 , pp. 42097, 2017, ISSN: 2045-2322 (Electronic) 2045-2322 (Linking).

Abstract | Links | BibTeX

63.

Rao, L.; Meng, Q. F.; Bu, L. L.; Cai, B.; Huang, Q.; Sun, Z. J.; Zhang, W. F.; Li, A.; Guo, S. S.; Liu, W.; Wang, T. H.; Zhao, X. Z.

Erythrocyte Membrane-Coated Upconversion Nanoparticles with Minimal Protein Adsorption for Enhanced Tumor Imaging Journal Article

In: ACS Appl Mater Interfaces, 9 (3), pp. 2159-2168, 2017, ISSN: 1944-8252 (Electronic) 1944-8244 (Linking).

Abstract | Links | BibTeX

64.

Zhang, Y.; Zhang, Y.; Burke, J. M.; Gleitsman, K.; Friedrich, S. M.; Liu, K. J.; Wang, T. H.

A Simple Thermoplastic Substrate Containing Hierarchical Silica Lamellae for High-Molecular-Weight DNA Extraction Journal Article

In: Adv Mater, 28 (48), pp. 10630-10636, 2016, ISSN: 1521-4095 (Electronic) 0935-9648 (Linking).

Abstract | Links | BibTeX

65.

Santos, J. L.; Ren, Y.; Vandermark, J.; Archang, M. M.; Williford, J. M.; Liu, H. W.; Lee, J.; Wang, T. H.; Mao, H. Q.

Continuous Production of Discrete Plasmid DNA-Polycation Nanoparticles Using Flash Nanocomplexation Journal Article

In: Small, 12 (45), pp. 6214-6222, 2016, ISSN: 1613-6829 (Electronic) 1613-6810 (Linking).

Abstract | Links | BibTeX

66.

Carleton, P. Ford; Schachter, S.; Parrish, J. A.; Collins, J. M.; Crocker, J. B.; Dixon, R. F.; Edgman-Levitan, S.; Lewandrowski, K. B.; Stahl, J. E.; Klapperich, C.; Cabodi, M.; Gaydos, C. A.; Rompalo, A. M.; Manabe, Y.; Wang, T. H.; Rothman, R.; Geddes, C. D.; Widdice, L.; Jackman, J.; Mathura, R. A.; Lash, T. B.

National Institute of Biomedical Imaging and Bioengineering Point-of-Care Technology Research Network: Advancing Precision Medicine Journal Article

In: IEEE J Transl Eng Health Med, 4 , pp. 2800614, 2016, ISSN: 2168-2372 (Print) 2168-2372 (Linking).

Abstract | Links | BibTeX

67.

Lao, Y. H.; Chi, C. W.; Friedrich, S. M.; Peck, K.; Wang, T. H.; Leong, K. W.; Chen, L. C.

Signal-on Protein Detection via Dye Translocation between Aptamer and Quantum Dot Journal Article

In: ACS Appl Mater Interfaces, 8 (19), pp. 12048-55, 2016, ISSN: 1944-8252 (Electronic) 1944-8244 (Linking).

Abstract | Links | BibTeX

68.

Rao, L.; Bu, L. L.; Cai, B.; Xu, J. H.; Li, A.; Zhang, W. F.; Sun, Z. J.; Guo, S. S.; Liu, W.; Wang, T. H.; Zhao, X. Z.

Cancer Cell Membrane-Coated Upconversion Nanoprobes for Highly Specific Tumor Imaging Journal Article

In: Adv Mater, 28 (18), pp. 3460-6, 2016, ISSN: 1521-4095 (Electronic) 0935-9648 (Linking).

Abstract | Links | BibTeX

69.

Friedrich, S. M.; Zec, H. C.; Wang, T. H.

Analysis of single nucleic acid molecules in micro- and nano-fluidics Journal Article

In: Lab Chip, 16 (5), pp. 790-811, 2016, ISSN: 1473-0189 (Electronic) 1473-0189 (Linking).

Abstract | Links | BibTeX

70.

Stark, A.; Shin, D. J.; 2nd Pisanic, T.; Hsieh, K.; Wang, T. H.

A parallelized microfluidic DNA bisulfite conversion module for streamlined methylation analysis Journal Article

In: Biomed Microdevices, 18 (1), pp. 5, 2016, ISSN: 1572-8781 (Electronic) 1387-2176 (Linking).

Abstract | Links | BibTeX

71.

Fraley, S. I.; Athamanolap, P.; Masek, B. J.; Hardick, J.; Carroll, K. C.; Hsieh, Y. H.; Rothman, R. E.; Gaydos, C. A.; Wang, T. H.; Yang, S.

Nested Machine Learning Facilitates Increased Sequence Content for Large-Scale Automated High Resolution Melt Genotyping Journal Article

In: Sci Rep, 6 , pp. 19218, 2016, ISSN: 2045-2322 (Electronic) 2045-2322 (Linking).

Abstract | Links | BibTeX

72.

Friedrich, S. M.; Liu, K. J.; Wang, T. H.

Single Molecule Hydrodynamic Separation Allows Sensitive and Quantitative Analysis of DNA Conformation and Binding Interactions in Free Solution Journal Article

In: J Am Chem Soc, 138 (1), pp. 319-27, 2016, ISSN: 1520-5126 (Electronic) 0002-7863 (Linking).

Abstract | Links | BibTeX

73.

2nd Pisanic, T. R.; Athamanolap, P.; Poh, W.; Chen, C.; Hulbert, A.; Brock, M. V.; Herman, J. G.; Wang, T. H.

DREAMing: a simple and ultrasensitive method for assessing intratumor epigenetic heterogeneity directly from liquid biopsies Journal Article

In: Nucleic Acids Res, 43 (22), pp. e154, 2015, ISSN: 1362-4962 (Electronic) 0305-1048 (Linking).

Abstract | Links | BibTeX

74.

Rao, L.; Bu, L. L.; Xu, J. H.; Cai, B.; Yu, G. T.; Yu, X.; He, Z.; Huang, Q.; Li, A.; Guo, S. S.; Zhang, W. F.; Liu, W.; Sun, Z. J.; Wang, H.; Wang, T. H.; Zhao, X. Z.

Red Blood Cell Membrane as a Biomimetic Nanocoating for Prolonged Circulation Time and Reduced Accelerated Blood Clearance Journal Article

In: Small, 11 (46), pp. 6225-36, 2015, ISSN: 1613-6829 (Electronic) 1613-6810 (Linking).

Abstract | Links | BibTeX

75.

Song, Y.; Liu, K. J.; Wang, T. H.

Efficient synthesis of stably adenylated DNA and RNA adapters for microRNA capture using T4 RNA ligase 1 Journal Article

In: Sci Rep, 5 , pp. 15620, 2015, ISSN: 2045-2322 (Electronic) 2045-2322 (Linking).

Abstract | Links | BibTeX

76.

Guan, W.; Chen, L.; Rane, T. D.; Wang, T. H.

Droplet Digital Enzyme-Linked Oligonucleotide Hybridization Assay for Absolute RNA Quantification Journal Article

In: Sci Rep, 5 , pp. 13795, 2015, ISSN: 2045-2322 (Electronic) 2045-2322 (Linking).

Abstract | Links | BibTeX

77.

Hsieh, Kuangwen; Zec, Helena; Ma, Polly; Rane, Tushar; Wang, Tza-Huei

Enhancing Throughput of Combinatorial Droplet Devices via Droplet Bifurcation, Parallelized Droplet Fusion, and Parallelized Detection Journal Article

In: Micromachines, 6 (10), pp. 1434, 2015, ISSN: 2072-666X.

Links | BibTeX

78.

Zhang, Y.; Wang, T. H.

High-resolution quantification by charge-dominant electrophoretic mobility shift of quantum dots Journal Article

In: Electrophoresis, 36 (7-8), pp. 1011-5, 2015, ISSN: 1522-2683 (Electronic) 0173-0835 (Linking).

Abstract | Links | BibTeX

79.

Rane, T. D.; Chen, L.; Zec, H. C.; Wang, T. H.

Microfluidic continuous flow digital loop-mediated isothermal amplification (LAMP) Journal Article

In: Lab Chip, 15 (3), pp. 776-82, 2015, ISSN: 1473-0189 (Electronic) 1473-0189 (Linking).

Abstract | Links | BibTeX

80.

Rane, T. D.; Zec, H. C.; Wang, T. H.

A barcode-free combinatorial screening platform for matrix metalloproteinase screening Journal Article

In: Anal Chem, 87 (3), pp. 1950-6, 2015, ISSN: 1520-6882 (Electronic) 0003-2700 (Linking).

Abstract | Links | BibTeX

152 entries « 4 of 8 »

152 entries « 4 of 4 »

2010

T. D. Rane; C. M. Puleo; K. J. Liu; Y. Zhang; A. P. Lee; T. H. Wang

Counting single molecules in sub-nanolitre droplets Journal Article

In: Lab Chip, 10 (2), pp. 161-4, 2010, ISSN: 1473-0197 (Print) 1473-0189 (Linking).

Abstract | Links | BibTeX

V. J. Bailey; B. P. Keeley; Y. Zhang; Y. P. Ho; H. Easwaran; M. V. Brock; K. L. Pelosky; H. E. Carraway; S. B. Baylin; J. G. Herman; T. H. Wang

Enzymatic incorporation of multiple dyes for increased sensitivity in QD-FRET sensing for DNA methylation detection Journal Article

In: Chembiochem, 11 (1), pp. 71-4, 2010, ISSN: 1439-7633 (Electronic) 1439-4227 (Linking).

Links | BibTeX

D. D. Nalayanda; Q. Wang; W. B. Fulton; T. H. Wang; F. Abdullah

Engineering an artificial alveolar-capillary membrane: a novel continuously perfused model within microchannels Journal Article

In: J Pediatr Surg, 45 (1), pp. 45-51, 2010, ISSN: 1531-5037 (Electronic) 0022-3468 (Linking).

Abstract | Links | BibTeX

2009

V. J. Bailey; C. M. Puleo; Y. P. Ho; H. C. Yeh; T. H. Wang

Quantum dots in molecular detection of disease Journal Article

In: Conf Proc IEEE Eng Med Biol Soc, 2009 , pp. 4089-92, 2009, ISSN: 1557-170X (Print) 1557-170X (Linking).

Abstract | Links | BibTeX

C. M. Puleo; W. McIntosh Ambrose; T. Takezawa; J. Elisseeff; T. H. Wang

Integration and application of vitrified collagen in multilayered microfluidic devices for corneal microtissue culture Journal Article

In: Lab Chip, 9 (22), pp. 3221-7, 2009, ISSN: 1473-0197 (Print) 1473-0189 (Linking).

Abstract | Links | BibTeX

D. D. Nalayanda; C. Puleo; W. B. Fulton; L. M. Sharpe; T. H. Wang; F. Abdullah

An open-access microfluidic model for lung-specific functional studies at an air-liquid interface Journal Article

In: Biomed Microdevices, 11 (5), pp. 1081-9, 2009, ISSN: 1572-8781 (Electronic) 1387-2176 (Linking).

Abstract | Links | BibTeX

Y. P. Ho; H. H. Chen; K. W. Leong; T. H. Wang

Combining QD-FRET and microfluidics to monitor DNA nanocomplex self-assembly in real-time Journal Article

In: J Vis Exp, (30), 2009, ISSN: 1940-087X (Electronic) 1940-087X (Linking).

Abstract | Links | BibTeX

V. J. Bailey; H. Easwaran; Y. Zhang; E. Griffiths; S. A. Belinsky; J. G. Herman; S. B. Baylin; H. E. Carraway; T. H. Wang

MS-qFRET: a quantum dot-based method for analysis of DNA methylation Journal Article

In: Genome Res, 19 (8), pp. 1455-61, 2009, ISSN: 1088-9051 (Print) 1088-9051 (Linking).

Abstract | Links | BibTeX

C. M. Puleo; T. H. Wang

Microfluidic means of achieving attomolar detection limits with molecular beacon probes Journal Article

In: Lab Chip, 9 (8), pp. 1065-72, 2009, ISSN: 1473-0197 (Print) 1473-0189 (Linking).

Abstract | Links | BibTeX

Y. Zhang; V. Bailey; C. M. Puleo; H. Easwaran; E. Griffiths; J. G. Herman; S. B. Baylin; T. H. Wang

DNA methylation analysis on a droplet-in-oil PCR array Journal Article

In: Lab Chip, 9 (8), pp. 1059-64, 2009, ISSN: 1473-0197 (Print) 1473-0189 (Linking).

Abstract | Links | BibTeX

H. H. Chen; Y. P. Ho; X. Jiang; H. Q. Mao; T. H. Wang; K. W. Leong

Simultaneous Non-invasive Analysis of DNA Condensation and Stability by Two-step QD-FRET Journal Article

In: Nano Today, 4 (2), pp. 125-134, 2009, ISSN: 1748-0132 (Print) 1748-0132 (Linking).

Abstract | Links | BibTeX

Y. P. Ho; H. H. Chen; K. W. Leong; T. H. Wang

The convergence of quantum-dot-mediated fluorescence resonance energy transfer and microfluidics for monitoring DNA polyplex self-assembly in real time Journal Article

In: Nanotechnology, 20 (9), pp. 095103, 2009, ISSN: 1361-6528 (Electronic) 0957-4484 (Linking).

Abstract | Links | BibTeX

2008

K. J. Liu; T. H. Wang

Cylindrical illumination confocal spectroscopy: rectifying the limitations of confocal single molecule spectroscopy through one-dimensional beam shaping Journal Article

In: Biophys J, 95 (6), pp. 2964-75, 2008, ISSN: 1542-0086 (Electronic) 0006-3495 (Linking).

Abstract | Links | BibTeX

H. C. Yeh; C. M. Puleo; Y. P. Ho; V. J. Bailey; T. C. Lim; K. Liu; T. H. Wang

Tunable blinking kinetics of cy5 for precise DNA quantification and single-nucleotide difference detection Journal Article

In: Biophys J, 95 (2), pp. 729-37, 2008, ISSN: 1542-0086 (Electronic) 0006-3495 (Linking).

Abstract | Links | BibTeX

C. M. Puleo; H. C. Yeh; K. J. Liu; T. H. Wang

Coupling confocal fluorescence detection and recirculating microfluidic control for single particle analysis in discrete nanoliter volumes Journal Article

In: Lab Chip, 8 (5), pp. 822-5, 2008, ISSN: 1473-0197 (Print) 1473-0189 (Linking).

Abstract | Links | BibTeX

T. C. Lim; V. J. Bailey; Y. P. Ho; T. H. Wang

Intercalating dye as an acceptor in quantum-dot-mediated FRET Journal Article

In: Nanotechnology, 19 (7), pp. 075701, 2008, ISSN: 0957-4484 (Print) 0957-4484 (Linking).

Abstract | Links | BibTeX

H. H. Chen; Y. P. Ho; X. Jiang; H. Q. Mao; T. H. Wang; K. W. Leong

Quantitative comparison of intracellular unpacking kinetics of polyplexes by a model constructed from quantum dot-FRET Journal Article

In: Mol Ther, 16 (2), pp. 324-32, 2008, ISSN: 1525-0024 (Electronic) 1525-0016 (Linking).

Abstract | Links | BibTeX

2007

C. M. Puleo; H. C. Yeh; T. H. Wang

Applications of MEMS technologies in tissue engineering Journal Article

In: Tissue Eng, 13 (12), pp. 2839-54, 2007, ISSN: 1076-3279 (Print) 1076-3279 (Linking).

Abstract | Links | BibTeX

S. Y. Chao; Y. P. Ho; V. J. Bailey; T. H. Wang

Quantification of low concentrations of DNA using single molecule detection and velocity measurement in a microchannel Journal Article

In: J Fluoresc, 17 (6), pp. 767-74, 2007, ISSN: 1053-0509 (Print) 1053-0509 (Linking).

Abstract | Links | BibTeX

D. D. Nalayanda; C. M. Puleo; W. B. Fulton; T. H. Wang; F. Abdullah

Characterization of pulmonary cell growth parameters in a continuous perfusion microfluidic environment Journal Article

In: Exp Lung Res, 33 (6), pp. 321-35, 2007, ISSN: 0190-2148 (Print) 0190-2148 (Linking).

Abstract | Links | BibTeX

2006

H. C. Yeh; C. M. Puleo; T. C. Lim; Y. P. Ho; P. E. Giza; R. C. Huang; T. H. Wang

A microfluidic-FCS platform for investigation on the dissociation of Sp1-DNA complex by doxorubicin Journal Article

In: Nucleic Acids Res, 34 (21), pp. e144, 2006, ISSN: 1362-4962 (Electronic) 0305-1048 (Linking).

Abstract | Links | BibTeX

Y. P. Ho; H. H. Chen; K. W. Leong; T. H. Wang

Evaluating the intracellular stability and unpacking of DNA nanocomplexes by quantum dots-FRET Journal Article

In: J Control Release, 116 (1), pp. 83-9, 2006, ISSN: 0168-3659 (Print) 0168-3659 (Linking).

Abstract | Links | BibTeX

C. M. Puleo; K. Liu; T. H. Wang

Pushing miRNA quantification to the limits: high-throughput miRNA gene expression analysis using single-molecule detection Journal Article

In: Nanomedicine (Lond), 1 (1), pp. 123-7, 2006, ISSN: 1748-6963 (Electronic) 1743-5889 (Linking).

Links | BibTeX

H. C. Yeh; Y. P. Ho; M. Shih Ie; T. H. Wang

Homogeneous point mutation detection by quantum dot-mediated two-color fluorescence coincidence analysis Journal Article

In: Nucleic Acids Res, 34 (5), pp. e35, 2006, ISSN: 1362-4962 (Electronic) 0305-1048 (Linking).

Abstract | Links | BibTeX

2005

H. C. Yeh; S. Y. Chao; Y. P. Ho; T. H. Wang

Single-molecule detection and probe strategies for rapid and ultrasensitive genomic detection Journal Article

In: Curr Pharm Biotechnol, 6 (6), pp. 453-61, 2005, ISSN: 1389-2010 (Print) 1389-2010 (Linking).

Abstract | Links | BibTeX

C. Y. Zhang; H. C. Yeh; M. T. Kuroki; T. H. Wang

Single-quantum-dot-based DNA nanosensor Journal Article

In: Nat Mater, 4 (11), pp. 826-31, 2005, ISSN: 1476-1122 (Print) 1476-1122 (Linking).

Abstract | Links | BibTeX

Y. P. Ho; M. C. Kung; S. Yang; T. H. Wang

Multiplexed hybridization detection with multicolor colocalization of quantum dot nanoprobes Journal Article

In: Nano Lett, 5 (9), pp. 1693-7, 2005, ISSN: 1530-6984 (Print) 1530-6984 (Linking).

Abstract | Links | BibTeX

H. C. Yeh; Y. P. Ho; T. H. Wang

Quantum dot-mediated biosensing assays for specific nucleic acid detection Journal Article

In: Nanomedicine, 1 (2), pp. 115-21, 2005, ISSN: 1549-9642 (Electronic) 1549-9634 (Linking).

Abstract | Links | BibTeX

T. H. Wang; Y. Peng; C. Zhang; P. K. Wong; C. M. Ho

Single-molecule tracing on a fluidic microchip for quantitative detection of low-abundance nucleic acids Journal Article

In: J Am Chem Soc, 127 (15), pp. 5354-9, 2005, ISSN: 0002-7863 (Print) 0002-7863 (Linking).

Abstract | Links | BibTeX

C. Y. Zhang; S. Y. Chao; T. H. Wang

Comparative quantification of nucleic acids using single-molecule detection and molecular beacons Journal Article

In: Analyst, 130 (4), pp. 483-8, 2005, ISSN: 0003-2654 (Print) 0003-2654 (Linking).

Abstract | Links | BibTeX

2004

P. K. Wong; C. Y. Chen; T. H. Wang; C. M. Ho

Electrokinetic bioprocessor for concentrating cells and molecules Journal Article

In: Anal Chem, 76 (23), pp. 6908-14, 2004, ISSN: 0003-2700 (Print) 0003-2700 (Linking).

Abstract | Links | BibTeX

P. K. Wong; T. H. Wang; J. H. Deval; C. M. Ho

Electrokinetics in Micro Devices for Biotechnology Applications Journal Article

In: IEEE/ASME Transactions on Mechatronics, 9 (2), pp. 366-376, 2004, ISSN: 1083-4435.

Links | BibTeX

152 entries « 4 of 4 »

Top

Patents

  1. H. Wang, F.M. Friedrich, J.M. Burke, and K. Liu “Concentration and Accumulation of Target Species in Response to a Gradient of Solute or Solvent” (JHU Ref. C13791; US provisional patent)
  2. H. Wang, K. Hsieh, H. Zec, L. Liu, A.M. Kaushik, Y. Yun, “Multiplexed, Continuous-flow, droplet-based platform for high-throughput genetic detection” (JHU Ref. C13529; US provisional patent)
  3. H. Wang, T.D. Rane, H.C Zec, “System and device for high throughput generation of combinatorial droplets and methods of use”  (PCT/US2015/012927; JHU Ref. C12862)
  4. H. Wang, D.J. Shin, “Self-contained cartridge and methods for integrated biochemical assay at the point-of-care” (JHU Ref. C12720)
  5. -H. Wang, S. Yang, M.A. Jacobs, P. Athamanolap, S.I. Fraley, V. Agarwal, V. Parekh. “Melt curve classifier for reliable large-scale genotyping of sequence variants” JHU Case Number C12600, provisional patent
  6. Stephanie I. Fraley, T.-H. Wang, and S. Yang. “A novel technology for broad-based, yet single molecule sensitive profiling in heterogeneous biological samples.” JHU Case Number C12403, provisional patent
  7. H. Wang, Y. Zhang, “Fabrication of Hierarchical Silica Nanomembrane using Heat-Shrinking Polymers and Applications of Silica Nanomembrane for the Solid Phase Extraction of Nucleic Acids”, JHU Case Number C12404, provisional patent
  8. H. Wang, Chi-Hang Chiou, Dong Jin Shin , “Electromagnetically Actuated Droplet Microfluidic Chip and System” (JHU Ref. C12262)
  9. H. Wang and Y. Zhang, “Surface Energy Traps (SETs) Enabled Complex Droplet Manipulation” (JHU Ref. C11971)
  10. H. Wang, K. Liu and Y. Song, “miRNA Analysis Method” (US Provisional US 61/598513, JHU Ref. C11885)
  11. H. Wang, T.D. Rane, H. C. Zec and W.Ch. Chu “Systems and methods for screening a library of samples” (US13/708,510. JHU Ref. C11803)
  12. Yang, T.H. Wang, S.K. Park and Y Zhang, “Method and apparatus for continuous microfluidic sample separation and concentration using AC electric field” (JHU Ref. C11562)
  13. H. Wang, Y. Zhang, S. Park and S. Yang, “Self-sustained Droplet Microfluidic Chip and System for Integrated Sample Preparation and Nucleic Acids Detection” (PCT/US2011/045363, JHU Ref. C11183)
  14. H. Wang, H.Q. Mao, W. Beh and D. Kraitchman, “Kilohertz Generation of Monodisperse Microbeads on a Polymeric Membrane Microfluidic Pseudocheck Valve Device” (PCT/US2011/054598, JHU Ref. C11248)
  15. Ahuja, V. Bailey, S.B. Baylin, J.G. Herman, T.H. Wang, J. Yi, “Early Detection of DNA Methylation Biomarker in Cancer Patient Sera” (PCT/US2011/037926, JHU Ref. C11091)
  16. H. Wang and K. Liu, “Hydrodynamic Particle Separation and Detection Systems and Methods”, (PCT/US2011/056941, JHU Ref. C11263)
  17. H. Wang and K.J. Liu, “Single Molecule Spectroscopy for Analysis of Cell-free Nucleic Acid Biomarkers” (PCT/US2010/033888, JHU Ref. C10750)
  18. H. Wang and Y. Zhang, ” Device and Method of Preparing and Performing Multiple Polymerase Chain Reactions” (US 12/716,031, JHU Ref. C10659)
  19. H. Wang, S. Baylin, J. Herman, H. Easwaran and H. Carraway, “Compositions and Methods for Polynucleotide Extraction and Methylation Detection” (PCT/US2009/000039, JHU Ref. C10249)
  20. M. Ho and T.H. Wang, “Biosensors and Methods for Their Use” (PCT/US2001/025444)
  21. H. Wang, K.J. Liu, and I.M. Shih, ” DNA Integrity Assay (DIA) for Cancer Diagnostics, Using Confocal Fluorescence Spectroscopy” (US8,835,110, JHU Ref. C10534)
  22. H. Wang and K. Liu, “Cylindrical Illumination Confocal Spectroscopy System” (US8,248,609, JHU Ref. C10398)
  23. H. Wang, K.J. Liu, C.M. Puleo and T. Rane, ” Microfluidic System for High-Throughput, Droplet-based, Single Molecule Analysis with Low Reagent Consumption” (US9,284,601, issued on 03/15/2016, JHU Ref. C10662)
  24. F. Miller, J. Huang, T.H. Wang, C.M. Ho and M. Liu, “Electrochemical Detection of Mismatch in Nucleic Acids” (US 7,291,457)
  25. H. Wang, “Method for determining standard cycle time of a stage dynamically” (US5,825,650)
  26. A. Hulbert, J. Herman, M. Brock, T.H. Wang, and A. Stark “Early detection of Individuals at High Risk for Lung Cancer of all Stages Using Gene promoter Methylation in Plasma and Sputum” JHU C13599

Top

Conference Publications

  1. J. Shin, P. Athamanolap, L.Chen, J. Hardick, CA Gaydos and T.H. Wang, “A Smartphone-Based Mobile NAAT Diagnostic Suite for Chlamydia Detection”, Proc. 26th Anniversary World Congress on Biosensors (Biosensors 2016), (accepted)
  2. M. Friedrich, J.M. Burke, K.J. Liu, T.H. Wang, “In-line Preconcentration, Size Separation, and Single-Molecule Detection Without Applied Electric Fields, Proc. 29th IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2016), p. 181-184, 2016
  3. J. Shin, L. Chen and T.H. Wang, “Single-bacteria Confocal Spectroscopy: An Ultrasensitive method for Real-time Monitoring of Bacterial Growth”, Proc. 19th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2015), p. 340-342, 2015
  4. Kaushik, K. Hsieh, L. Chen, D.J. Shin and T.H. Wang, “Rapid Assessment of Bacterial Vitality and Antibiotic Susceptibility via High-Throughput Picoliter-Droplet Single-Cell Assay”, Proc. 19th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2015), p. 531-533, 2015
  5. Liu, K. Hsieh, A. Kaushik, H.C. Zec and T.H. Wang, “Multiplexed, Continuous-Flow, Droplet-Based PCR Genotyping Platform for High-Throughput Agriculture Marker Assisted Selection”, Proc. 19th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2015), p. 1368-1370, 2015
  6. Zec, C. O’Keefe, P. Ma, T.H. Wang, “Ultra-Thin, Evaporation-Resistant PDMS Devices for Absolute Quantification of DNA Using Digital PCR”, The 18th International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers 2015), p.536-539, 2015
  7. Guan, L. Chen, T. Rane, A. Kaushik, T.H. Wang, “Digital Droplet ELOHA For Nucleic Molecule Counting And Analysis”, The 18th International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers 2015), p.536-5460-463, 2015
  8. C. Zec, C.J. Glover, W. Hsieh, L. Liu, C. O’Keefe and T.H. Wang, “Methods for Controlling Water Evaporation in PDMS-Based Microfluidic Devices”, Proc. 18th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2014), p. 1743-1745, 2014
  9. J. Shin, P. Athamanonolap, L. Chen and T.H. Wang, “Integrated droplet microfluidic platform for nucleic acids amplification test of Chlamydia trachomatis infection”, Proc. 24th Anniversary World Congress on Biosensors (Biosensors 2014)
  10. C. Zec, T.D. Rane, P. Ma and T.H. Wang, “Parallelization of Fission and Fusion- Operations for High Throughput Generation of Combinatorial Droplets”, Proc. 27th IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2014), p. 334-337, 2014
  11. J. Shin, L. Chen and T.H. Wang, “A Simple Integrated Diagnostic Platform for DNA Testing of Chlamydia Trachomatis Infection, “ Proc. 17th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2013), p. 1350-1352, 2013
  12. M. Friedrich, K.J. Liu and T.H. Wang, “ Single Molecule Hydrodynamic Separation for Ultrasensitive and Quantitative DNA Size Separations,” Proc. 17th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2013), p. 35-37, 2013
  13. D. Rane, H.C. Zec and T.H. Wang, “A Multiplexed Microfluidic Droplet Platform for Matrix Metalloproteinase Screening”, Proc. 17th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2013), p. 1595-1597, 2013
  14. K. Wu, S. F. Friedrich, K.J. Liu and T.H. Wang, “ Chip-Based DNA Separation in Free Solution by Inertial Hydrodynamic Forces”, Proc. 17th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2013), p. 578-580, 2013
  15. J. Shin, A. Stark and T.H. Wang, “ Droplet Bisulfite Conversion Platform for Epigenetic Cancer Biomarker Detection”, The 17th International Conference on Solid-State Sensors, Actuators and Microsystems(Transducers 2013), p.2181-2184, 2013
  16. Zhang, Y. Zhang, B. Keeley, A. Stark and T.H. Wang, “Spontaneous Shrinking Silica Nanomembrane for Solid Phase”, Proc. 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (IEEE NEMS 2013), p. 444-445, 2013
  17. Athamanolap, B. Keeley, D.J. Shin and T.H. Wang, “Quantitative Analysis of DNA Methylation Based on Melting Curve Analysis”, Proc. 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (IEEE NEMS 2013), p.1116-1118, 2013
  18. Zhang, Y Zhang, T.H. Wang, “Hierarchical Silica Nanomembrane Driven by Thermal Shrinkage and its Application for Solid Phase DNA Extraction”, Proc. The 13th IEEE International Conference on Nanotechnology (IEEE NANO 2013), 2013
  19. C. Zec, T.D. Rane, W.C. Chu, V. Wang and T.H. Wang, “ A Microfluidic Droplet Platform for Multiplexed Single Nucleotide Polymorphism Analysis of an Array Plant Genomic DNA Samples”, Proc. 26th IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2013), p.263-266, 2013
  20. H. Chiou, D.J. Shin, S. Hosmane, Y Zhang and T.H. Wang, “Electromagnet-Actuated Droplet Platform for Sample-to-Answer Genetic Detection”, Proc. 26th IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2013), p.98-101, 2013
  21. Zhang and T.H. Wang, “ All-in-One Droplet Platform for Multiplexed Genetic Detection in Blood” Proc. 26th IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2013), p. 1061-1064, 2013
  22. Zhang and T.H. Wang, “Flip-Drop: Droplet Array Created by Surface Energy Trap for Combinatorial Screening,” Proc. 26th IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2013), p.1149-1152, 2013
  23. W. Beh, C. Weiss, H.Q. Mao, D. L. Kraitchman, and T.H. Wang, “High-Throughput Microfluidic Preparation of Imaging-Visible Embolic Beads”, Proc. EMBS Micro and Nanotechnology in Medicine Conference, p.59, 2012
  24. Zhang, Y. Zhang, B. Keeley, A. Stark and T.H. Wang, “Fabricating and Applying Hierarchical Silica Nanomembrane with for Solid Phase DNA Extraction” The 6th IEEE International Conference on Nano/Molecular Medicine and Engineering (IEEE NANOMED 2012), 2012
  25. Keeley, Yi Zhang, Ye Zhang, A. Stark, T.H. Wang, “Quantum Dot FRET Linker Probes for Highly Sensitive DNA Methylation Detection”, Proc. IEEE 12th International Conference on Nanotechnology (IEEE NANO 2012), 7848, p.1-4, 2012
  26. Zhang and T.H. Wang, “Quantum Dot Electrophoretic Mobility Shift Assay and Its Application to the Measurement of Exonuclease Activity” Proc. IEEE 12th International Conference on Nanotechnology (IEEE NANO 2012), 7685, p.1-4, 2012
  27. Song, L. Zhang, M. Chen and T.H. Wang, “Single Quantum Dot Fluorescence Enhancement by Tunable Nanoporous Gold”, Proc. IEEE 12th International Conference on Nanotechnology (IEEE NANO 2012), 7877, p.1-4, 2012
  28. Zhang and T.H. Wang, “Droplet Immobilization, Splitting, Metering and Aliquoting with Surface Energy Traps Created Using SU8 Shadow Mask” Proc. 16th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2012), p. 73-75, 2012
  29. Zhang and T.H. Wang, “Surface Energy Trap Assisted Rapid Serial Dilution on Droplet Platform for Bacteria Antibiotics Susceptibility Test” Proc. 16th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2012), p. 467-469, 2012
  30. J. Liu, T.D. Rane, Y. Zhang, C. Beh, D.J. Shin, T.H. Wang, “An Integrated Platform for Single Molecule Free Solution Hydrodynamic Separation Using Yoctomoles of DNA and Picoliter Samples”, ASME 10th International Conference on Nanochannels, Microchannels and Minichannels (ICNMM 2012), ICNMM2012-73154 (p.1-6), 2012
  31. Zec, T.D. Rane, W.C. Chu and T.H. Wang, “Multiplexed Screening of a Large Library of Biological Samples through on-Demand Droplet Generation and Fusion”, ASME 2012 10th International Conference on Nanochannels, Microchannels and Minichannels (ICNMM 2012), ICNMM2012-73159 (p.1-6), 2012
  32. Song, Y. Zhang, and T.H. Wang, “Single Quantum Dot-Based Multiplexed Point Mutation Detection by Gap Ligase Chain Reaction”, Proc. 15th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2011), p.1779-1781, 2011
  33. Zhang, D.J. Shin and T.H. Wang, “Detecting Genetic Variations in A Droplet”, Proc. 15th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2011), p.1179-1181, 2011
  34. J. Liu and T.H. Wang, “ PCR-free, microfluidic single molecule analysis of circulating nucleic acids in lung cancer patient serum”, Proc. 33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC ’11), p.8392-8395, 2011
  35. Zhang and T.H. Wang, “ An Active Gyroscopic Magnetic Micromixer for Rapid Fluid Mixing in Droplet Based Microfluidic Systems”, The 16th International Conference on Solid-State Sensors, Actuators and Microsystems(Transducers 2011), p.1769-1772, 2011
  36. Zhang, S.K. Park, S. Yang and T.H. Wang, “Fully Integrated Droplet Based Point-of-Care Platform for Molecular Detection from Crude Biosamples”, The 16th International Conference on Solid-State Sensors, Actuators and Microsystems(Transducers 2011), p.1927-1930, 2011
  37. H. Wang, V. Bailey and K. Liu, “Quantum Dots and Microfluidic Single Molecule Detection for Screening Genetic and Epigenetic Cancer Markers in Clinical Samples”, Proc. 2011 SPIE Defense, Security and Sensing Conference, Volume 8031, P. 80311W
  38. D. Rane, H. Zec, C.M. Puleo, A.P. Lee and T.H. Wang, “High-Throughput Single-Cell Pathogen Detection on a Droplet Microfluidic Platform”, Proc. 24th IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2011), p.881-884, 2011
  39. Stark, Y. Zhang, V. Bailey, B. Keeley. T.H. Wang, “Increasing Throughput and Sensitivity of DNA Methylation Analysis through Functional Nanoparticles” The 6th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (IEEE NEMS 2011), 1091-1094, 2011
  40. Y Zhang and T.H. Wang, “A Quantum Dot Based Electrophoretic Mobility Shift Assay for High Resolution Copy Number Variation Study”, The 6th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (IEEE NEMS 2011), p.841-844, 2011
  41. Zhang, I.M. Shih, T.L. Wang and T.H. Wang, ” A Quantum Dot Based Nanoassay for Quantifying Gene Copy Number with Ultrahigh Resolution”, Proc. 14th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2010), p.1154-1156, 2010
  42. W. Beh, W. Zhou and T.H. Wang, “Oxygen Plasma-Free Microfluidic Device Sealing”, Proc. 14th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2010) p.1217-1219, 2010
  43. W. Beh, D. Kraitchman, H.Q. Mao, T.H. Wang, “High-throughput Monodisperse Alginate Gel Bead Formation using Microfluidic Pseudo-Check Valve”, Proc. 14th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2010) p.425-427, 2010
  44. Zhang and T.H. Wang, “An Automated All-in-one Microfluidic Device for Parallel Solid Phase DNA Extraction and Droplet-in-Oil PCR Analysis”, Proc. 23rd IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2010), P.971-974, 2010
  45. Zhang and T.H. Wang, “Geomorphology-Assisted Manopulation of Magnet-Actuated Droplet for Solid Phase DNA Extraction and Droplet-in-Oil PCR”, Proc. 23rd IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2010), p.1047-1050, 2010
  46. D. Rane, C.M. Puleo, H. Zec, Y. Zhang, A.P. Lee and T.H. Wamh, “Analyte Detection in Droplets: One Molecule at a Time”, Proc. 13th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2009), p.15-17, 2009
  47. M. Puleo, H.C. Zec, Y. Sung and T.H. Wang, “Micro-evaporator as Interconnects to Low-Volume Microfluidic Components”, Proc. 13th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2009), p.932-935, 2009
  48. J. Bailey, C.M. Puleo, Y.P. Ho, H.C. Yeh, T.H. Wang, “Quantum Dots in Molecular Detection of Diseases”, 31st Annual International Conference on the IEEE Engineering in Medicine and Biology Society ( IEEE EMBC 2009), p. 4089-4092, 2009
  49. Zhang, V. Bailey, C.M. Puleo, H. Easwaran, E. Griffiths, J.G. Herman, S.B. Baylin, T.H. Wang, “High Throughput DNA Methylation Analysis on a Droplet-in-Oil Polymerase Chain Reaction Array”, The 15th International Conference on Solid-State Sensors, Actuators and Microsystems(Transducers 2009), p.806-808, 2009
  50. Zhang, V. Bailey, C.M. Puleo, C. Chen and T.H. Wang, “Multiple gene Analysis within a Simple Droplet-in-Oil Microfluidic PCR Platform”, Proc. 12th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2008), p. 751-753 , 2008
  51. P. Ho, H.H. Chen, K. Leong and T.H. Wang “Quantitative Kinetic Analysis of DNA Nanocomplex Self-Assembly with Quantum Dots FRET in a Microfluidic Device”, Proc. 21st IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2008), p. 30-33, 2008
  52. M. Puleo, H.C. Yeh and T.H. Wang “Single Molecule Detection in Truly, Nanoliter-Sized Volumes: Coupling Evaporation-Based, Microfluidic Concentration with Confocal Fluorescence Spectroscopy” Proc. 21st IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2008), p.200-203, 2008
  53. Liu and T.H. Wang, “Quantitative Confocal Spectroscopy – Rectifying the Limitations of Single Molecule Detection”, Proc. 3rd Annual IEEE International Conferences on Nano/Micro Engineered and Molecular Systems (IEEE-NEMS 2008), p. 1189-1192, 2008
  54. C. Yeh. Y.P. Ho, C.M. Puleo and T.H. Wang,” Towards single-molecule diagnostics using microfluidic manipulation and quantum dot nanosensors, Proc. 5th International Conference on Nanochannels, Microchannels and Minichannels, (ICNMM 2007), p. 1133-1140, 2007
  55. P. Ho and T.H. Wang, “Multiplexed Detection of Anthrax Sequences with Quantum Dot Nanoprobes”, Proc. IEEE/NLM Life Science Systems and Application Workshop, p. 62-63, 2006
  56. Murray , K. Rebello, J. Crookston, J. Miragliotta and T.H. Wang, “High-degree Concentration of Bio-agents Using Electrokinetic Manipulations”, Proc. IEEE/NLM Life Science Systems and Application Workshop, p. 94-95, 2006
  57. P. Ho, M.C. Kung and T.H. Wang, ” Separation-free Detection of Low-abundant Biomolecules with Two-Color Colocalization of Quantum Dot Probes,” Proc. 9th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2005), p. 1330-1332, 2005
  58. C. Yeh, Y.P. Ho and T.H. Wang, “Quantum Dot-Mediated Separation-Free Assay for Point Mutation Detection ” Proc. NSTI- Bio-Nanotechnology Conference, 198-201, 2005
  59. C. Yeh, E. Simone, C.Y. Zhang and T.H. Wang, “Single Bio-Molecule Detection with Quantum-dots in Flow-rate Controlled Microchannel”, Proc. 17h IEEE Annual Workshop of Micro Electro Mechanical Systems (IEEE MEMS 2004), p 371-374, 2004
  60. Y. Chao, C.Y. Zhang and T.H. Wang,” Measurement of in-situ Flow Velocity Using Single-Molecule Detection for the Application of Biomolecule Quantification”, Proc. Hilton Head 2004 Solid-State Sensor, Actuator, and Microsystems Workshop (Hilton Head 2004), p. 176-179, 2004
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  64. K. Wong, T.H. Wang and C.M. Ho, “Optical Fiber Tip Fabricated by Surface Tension Controlled Etching”, Proc. Hilton Head 2002: Solid-State Sensor, Actuator, and Microsystems Workshop (Hilton Head 2002), p. 94-97, 2002
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