Journal Publications | Patents | Conference Publications

Journal Publications

139 entries « 1 of 7 »
1. Zhang, Y; Zhang, P; Chen, L; Kaushik, A; Hu, K; Wang, T H: ddRFC: A scalable multiplexed droplet digital nucleic acid amplification test platform. In: Biosensors and Bioelectronics, 167 (112499), 2020. (Type: Journal Article | Links | BibTeX)
2. Bell, C; Fang, W; Berlinicke, C; Kaushik, A; Zhang, P; Wang, T H; Kalhor, R; Ji, H; Zack, D J: Single-cell lineage tracing of developing retinal systems. In: Investigative Ophthalmology & Visual Science, 61 (7), pp. 4014, 2020. (Type: Journal Article | Links | BibTeX)
3. Li, S; Li, A; Hsieh, K; Friedrich, S M; Wang, T H: Electrode-free Concentration and Recovery of DNA at Physiologically Relevant Ionic Concentrations. In: Analytical Chemistry, 92 (8), pp. 6150–6157, 2020. (Type: Journal Article | Links | BibTeX)
4. Yang, X; Hashemi, M M; Andini, N; Li, M M; Kuang, S; Carroll, K C; Wang, T H; Yan, S: RNA markers for ultra-rapid molecular antimicrobial susceptibility testing in fluoroquinolone-treated Klebsiella pneumoniae. In: Journal of Antimicrobial Chemotherapy, 2020. (Type: Journal Article | Links | BibTeX)
5. Pisanic, T R; Wang, Y; Sun, H; Considine, M; Li, L; Wang, T H; Wang, T L; Shih, Ie M: The methylomic landscape of fallopian tube lesions associated with ovarian high-grade serous carcinoma. In: Clinical Cancer Research, 2020. (Type: Journal Article | Links | BibTeX)
6. Liu, B; Ricarte-Filho, J; Mallisetty, A; Villani, C; Kottorou, A E; Rodgers, K P; Chen, C; Ito, T; Holmes, K; Gastala, N; Valyi-Nagy, K; David, O; Gaba, R C; Ascoli, C; Pasquinelli, M; Feldman, L E; Massad, M G; Wang, T H; Jusue-Torres, I; Benedetti, E; Winn, R A; Brock, M V; Herman, J G; Hulbert, A: Detection of Promoter DNA Methylation in Urine and Plasma Aids the Detection of Non–Small Cell Lung Cancer. In: Clinical Cancer Research, 26 (16), 2020. (Type: Journal Article | Links | BibTeX)
7. Hsieh, K; Zhao, G; Wang, T H: Applying Biosensor Development Concepts to Improve Preamplification-Free CRISPR/Cas12a-Dx. In: Analyst, 145 , pp. 4880-4888, 2020. (Type: Journal Article | Links | BibTeX)
8. Kallman, A; Capowski, E E; Wang, J; Kaushik, A M; Jansen, A D; Edwards, K L; Chen, L; Berlinicke, C A; Phillips, M J; Pierce, E A; Qian, J; Wang, T H; Gamm, D M; Zack, D J: Investigating cone photoreceptor development using patient-derived NRL null retinal organoids. In: Communications Biology, 3 (1), pp. 1-13, 2020. (Type: Journal Article | Links | BibTeX)
9. Chen, B; Gao, T; Yuan, W; Zhao, W; Wang, T H; Wu, J: Prognostic Value of Survival of MicroRNAs Signatures in Non-small Cell Lung Cancer. In: Journal of Cancer, 10 (23), pp. 5793-5804, 2019. (Type: Journal Article | Links | BibTeX)
10. Athamanolap, P; Hsieh, K; O’Keefe, C M; Zhang, Y; Yang, S; Wang, T H: Nanoarray Digital Polymerase Chain Reaction with High-Resolution Melt for Enabling Broad Bacteria Identification and Pheno–Molecular Antimicrobial Susceptibility Test. In: Anal Chem, 91 (20), pp. 12784-12792, 2019. (Type: Journal Article | Links | BibTeX)
11. Chen, B; Huang, S; Pisanic, T R; Stark, A; Tao, Y; Cheng, B; Li, Y; Wei, Y; Zhao, W; Wang, T H; Wu, J: Rab8 GTPase regulates Klotho-mediated inhibition of cell growth and progression by directly modulating its surface expression in human non-small cell lung cancer. In: EBioMedicine, 49 , pp. 118-132, 2019. (Type: Journal Article | Links | BibTeX)
12. Loo, J F C; Ho, H P; Kong, S K; Wang, T H; Ho, Y P: Technological Advances in Multiscale Analysis of Single Cells in Biomedicine. In: Advanced Biosystems, 3 (11), pp. 1900138, 2019. (Type: Journal Article | Links | BibTeX)
13. O’Keefe, C M; Kaushik, A M; and Wang, T H: Highly Efficient Real-Time Droplet Analysis Platform for High-Throughput Interrogation of DNA Sequences by Melt. In: Anal Chem, 91 (17), pp. 11275-11282, 2019. (Type: Journal Article | Links | BibTeX)
14. Zhang, Y; Wang, T H: Rapid generation of chemical combinations on a magnetic digital microfluidic array. In: RSC Advances, 9 (38), pp. 21741-21747, 2019. (Type: Journal Article | Links | BibTeX)
15. Zhang, P; Kaushik, A; Hsieh, K; Wang, T H: Customizing droplet contents and dynamic ranges via integrated programmable picodroplet assembler. In: Microsystems & Nanoengineering, 5 (1), pp. 22, 2019, ISSN: 2055-7434. (Type: Journal Article | Abstract | Links | BibTeX)
16. Negron, K; Khalasawi, N; Lu, B; Ho, C Y; Lee, J; Shenoy, S; Mao, H Q; Wang, T H; Hanes, J; Suk, J S: Widespread gene transfer to malignant gliomas with In vitro-to-In vivo correlation. In: J Control Release, 2019, ISSN: 1873-4995 (Electronic) 0168-3659 (Linking). (Type: Journal Article | Abstract | Links | BibTeX)
17. Eissa, M A L; Lerner, L; Abdelfatah, E; Shankar, N; Canner, J K; Hasan, N M; Yaghoobi, V; Huang, B; Kerner, Z; Takaesu, F; Wolfgang, C; Kwak, R; Ruiz, M; Tam, M; Pisanic, 2nd T R; Iacobuzio-Donahue, C A; Hruban, R H; He, J; Wang, T H; Wood, L D; Sharma, A; Ahuja, N: Promoter methylation of ADAMTS1 and BNC1 as potential biomarkers for early detection of pancreatic cancer in blood. In: Clin Epigenetics, 11 (1), pp. 59, 2019, ISSN: 1868-7083 (Electronic) 1868-7075 (Linking). (Type: Journal Article | Abstract | Links | BibTeX)
18. Shin, D J; Andini, N; Hsieh, K; Yang, S; Wang, T H: Emerging Analytical Techniques for Rapid Pathogen Identification and Susceptibility Testing. In: Annu Rev Anal Chem (Palo Alto Calif), 2019, ISSN: 1936-1335 (Electronic) 1936-1327 (Linking). (Type: Journal Article | Abstract | Links | BibTeX)
19. Wang, Z; Kambhampati, S; Cheng, Y; Ma, K; Simsek, C; Tieu, A H; Abraham, J M; Liu, X; Prasath, V; Duncan, M; Stark, A; Trick, A; Tsai, H L; Wang, H; He, Y; Khashab, M A; Ngamruengphong, S; Shin, E J; Wang, T H; Meltzer, S J: Methylation Biomarker Panel Performance in EsophaCap Cytology Samples for Diagnosing Barrett's Esophagus: A Prospective Validation Study. In: Clin Cancer Res, 25 (7), pp. 2127-2135, 2019, ISSN: 1078-0432 (Print) 1078-0432 (Linking). (Type: Journal Article | Abstract | Links | BibTeX)
20. Pisanic, T R; Wang, Y; Sun, H; Considine, M; L. Li, Wang T H; Wang, T L; Shih, I M: Long Interspersed Nuclear Element 1 Retrotransposons Become Deregulated during the Development of Ovarian Cancer Precursor Lesions. In: Am J Pathol, 189 (3), pp. 513-520, 2019, ISSN: 1525-2191 (Electronic) 0002-9440 (Linking). (Type: Journal Article | Abstract | Links | BibTeX)
139 entries « 1 of 7 »

139 entries « 1 of 4 »

2020

Y. Zhang; P. Zhang; L. Chen; A. Kaushik; K. Hu; T.H. Wang

ddRFC: A scalable multiplexed droplet digital nucleic acid amplification test platform Journal Article

Biosensors and Bioelectronics, 167 (112499), 2020.

Links | BibTeX

C. Bell; W. Fang; C. Berlinicke; A. Kaushik; P. Zhang; T.H. Wang; R. Kalhor; H. Ji; D.J. Zack

Single-cell lineage tracing of developing retinal systems Journal Article

Investigative Ophthalmology & Visual Science, 61 (7), pp. 4014, 2020.

Links | BibTeX

S. Li; A. Li; K. Hsieh; S.M. Friedrich; T.H. Wang

Electrode-free Concentration and Recovery of DNA at Physiologically Relevant Ionic Concentrations Journal Article

Analytical Chemistry, 92 (8), pp. 6150–6157, 2020.

Links | BibTeX

X. Yang; M.M. Hashemi; N. Andini; M.M. Li; S. Kuang; K.C. Carroll; T.H. Wang; S. Yan

RNA markers for ultra-rapid molecular antimicrobial susceptibility testing in fluoroquinolone-treated Klebsiella pneumoniae Journal Article

Journal of Antimicrobial Chemotherapy, 2020.

Links | BibTeX

T R Pisanic; Y Wang; H Sun; M Considine; L Li; T H Wang; T L Wang; Ie M Shih

The methylomic landscape of fallopian tube lesions associated with ovarian high-grade serous carcinoma Journal Article

Clinical Cancer Research, 2020.

Links | BibTeX

B. Liu; J. Ricarte-Filho; A. Mallisetty; C. Villani; A.E. Kottorou; K.P. Rodgers; C. Chen; T. Ito; K. Holmes; N. Gastala; K. Valyi-Nagy; O. David; R.C. Gaba; C. Ascoli; M. Pasquinelli; L.E. Feldman; M.G. Massad; T.H. Wang; I. Jusue-Torres; E. Benedetti; R.A. Winn; M.V. Brock; J.G. Herman; A. Hulbert

Detection of Promoter DNA Methylation in Urine and Plasma Aids the Detection of Non–Small Cell Lung Cancer Journal Article

Clinical Cancer Research, 26 (16), 2020.

Links | BibTeX

K. Hsieh; G. Zhao; T.H. Wang

Applying Biosensor Development Concepts to Improve Preamplification-Free CRISPR/Cas12a-Dx Journal Article

Analyst, 145 , pp. 4880-4888, 2020.

Links | BibTeX

A. Kallman; E.E. Capowski; J. Wang; A.M. Kaushik; A.D. Jansen; K.L. Edwards; L. Chen; C.A. Berlinicke; M.J. Phillips; E.A. Pierce; J. Qian; T.H. Wang; D.M. Gamm; D.J. Zack

Investigating cone photoreceptor development using patient-derived NRL null retinal organoids Journal Article

Communications Biology, 3 (1), pp. 1-13, 2020.

Links | BibTeX

2019

B Chen; T Gao; W Yuan; W Zhao; T H Wang; J Wu

Prognostic Value of Survival of MicroRNAs Signatures in Non-small Cell Lung Cancer Journal Article

Journal of Cancer, 10 (23), pp. 5793-5804, 2019.

Links | BibTeX

P. Athamanolap; K. Hsieh; C.M. O’Keefe; Y. Zhang; S. Yang; T.H. Wang

Nanoarray Digital Polymerase Chain Reaction with High-Resolution Melt for Enabling Broad Bacteria Identification and Pheno–Molecular Antimicrobial Susceptibility Test Journal Article

Anal Chem, 91 (20), pp. 12784-12792, 2019.

Links | BibTeX

B Chen; S Huang; T R Pisanic; A Stark; Y Tao; B Cheng; Y Li; Y Wei; W Zhao; T H Wang; J Wu

Rab8 GTPase regulates Klotho-mediated inhibition of cell growth and progression by directly modulating its surface expression in human non-small cell lung cancer Journal Article

EBioMedicine, 49 , pp. 118-132, 2019.

Links | BibTeX

J F C Loo; H P Ho; S K Kong; T H Wang; Y P Ho

Technological Advances in Multiscale Analysis of Single Cells in Biomedicine Journal Article

Advanced Biosystems, 3 (11), pp. 1900138, 2019.

Links | BibTeX

C.M. O’Keefe; A.M. Kaushik; and T.H. Wang

Highly Efficient Real-Time Droplet Analysis Platform for High-Throughput Interrogation of DNA Sequences by Melt Journal Article

Anal Chem, 91 (17), pp. 11275-11282, 2019.

Links | BibTeX

Y Zhang; T H Wang

Rapid generation of chemical combinations on a magnetic digital microfluidic array Journal Article

RSC Advances, 9 (38), pp. 21741-21747, 2019.

Links | BibTeX

P Zhang; A Kaushik; K Hsieh; T H Wang

Customizing droplet contents and dynamic ranges via integrated programmable picodroplet assembler Journal Article

Microsystems & Nanoengineering, 5 (1), pp. 22, 2019, ISSN: 2055-7434.

Abstract | Links | BibTeX

K. Negron; N. Khalasawi; B. Lu; C. Y. Ho; J. Lee; S. Shenoy; H. Q. Mao; T. H. Wang; J. Hanes; J. S. Suk

Widespread gene transfer to malignant gliomas with In vitro-to-In vivo correlation Journal Article

J Control Release, 2019, ISSN: 1873-4995 (Electronic) 0168-3659 (Linking).

Abstract | Links | BibTeX

M. A. L. Eissa; L. Lerner; E. Abdelfatah; N. Shankar; J. K. Canner; N. M. Hasan; V. Yaghoobi; B. Huang; Z. Kerner; F. Takaesu; C. Wolfgang; R. Kwak; M. Ruiz; M. Tam; T. R. 2nd Pisanic; C. A. Iacobuzio-Donahue; R. H. Hruban; J. He; T. H. Wang; L. D. Wood; A. Sharma; N. Ahuja

Promoter methylation of ADAMTS1 and BNC1 as potential biomarkers for early detection of pancreatic cancer in blood Journal Article

Clin Epigenetics, 11 (1), pp. 59, 2019, ISSN: 1868-7083 (Electronic) 1868-7075 (Linking).

Abstract | Links | BibTeX

D. J. Shin; N. Andini; K. Hsieh; S. Yang; T. H. Wang

Emerging Analytical Techniques for Rapid Pathogen Identification and Susceptibility Testing Journal Article

Annu Rev Anal Chem (Palo Alto Calif), 2019, ISSN: 1936-1335 (Electronic) 1936-1327 (Linking).

Abstract | Links | BibTeX

Z. Wang; S. Kambhampati; Y. Cheng; K. Ma; C. Simsek; A. H. Tieu; J. M. Abraham; X. Liu; V. Prasath; M. Duncan; A. Stark; A. Trick; H. L. Tsai; H. Wang; Y. He; M. A. Khashab; S. Ngamruengphong; E. J. Shin; T. H. Wang; S. J. Meltzer

Methylation Biomarker Panel Performance in EsophaCap Cytology Samples for Diagnosing Barrett's Esophagus: A Prospective Validation Study Journal Article

Clin Cancer Res, 25 (7), pp. 2127-2135, 2019, ISSN: 1078-0432 (Print) 1078-0432 (Linking).

Abstract | Links | BibTeX

T.R. Pisanic; Y. Wang; H. Sun; M. Considine; L. Li, T.H. Wang; T.L. Wang; I.M. Shih

Long Interspersed Nuclear Element 1 Retrotransposons Become Deregulated during the Development of Ovarian Cancer Precursor Lesions Journal Article

Am J Pathol, 189 (3), pp. 513-520, 2019, ISSN: 1525-2191 (Electronic) 0002-9440 (Linking).

Abstract | Links | BibTeX

J. Gao; H. Li; P. Torab; K. E. Mach; D. W. Craft; N. J. Thomas; C. M. Puleo; J. C. Liao; T. H. Wang; P. K. Wong

Nanotube assisted microwave electroporation for single cell pathogen identification and antimicrobial susceptibility testing Journal Article

Nanomedicine, 17 , pp. 246-253, 2019, ISSN: 1549-9642 (Electronic) 1549-9634 (Linking).

Abstract | Links | BibTeX

S. M. Friedrich; R. Bang; A. Li; T. H. Wang

Versatile Analysis of DNA-Biomolecule Interactions in Solution by Hydrodynamic Separation and Single Molecule Detection Journal Article

Anal Chem, 91 (4), pp. 2822-2830, 2019, ISSN: 1520-6882 (Electronic) 0003-2700 (Linking).

Abstract | Links | BibTeX

C. M. O'Keefe; D. Giammanco; S. Li; T. R. Pisanic; T. J. Wang

Multilayer microfluidic array for highly efficient sample loading and digital melt analysis of DNA methylation Journal Article

Lab Chip, 19 (3), pp. 444-451, 2019, ISSN: 1473-0189 (Electronic) 1473-0189 (Linking).

Abstract | Links | BibTeX

K. E. Mach; A. M. Kaushik; K. Hsieh; P. K. Wong; T. H. Wang; J. C. Liao

Optimizing peptide nucleic acid probes for hybridization-based detection and identification of bacterial pathogens Journal Article

Analyst, 2019, ISSN: 1364-5528 (Electronic) 0003-2654 (Linking).

Abstract | Links | BibTeX

2018

T. R. 2nd Pisanic; L. M. Cope; S. F. Lin; T. T. Yen; P. Athamanolap; R. Asaka; K. Nakayama; A. N. Fader; T. H. Wang; I. M. Shih; T. L. Wang

Methylomic Analysis of Ovarian Cancers Identifies Tumor-Specific Alterations Readily Detectable in Early Precursor Lesions Journal Article

Clin Cancer Res, 24 (24), pp. 6536-6547, 2018, ISSN: 1078-0432 (Print) 1078-0432 (Linking).

Abstract | Links | BibTeX

C. W. Beh; Y. Zhang; Y. L. Zheng; B. Sun; T. H. Wang

Fluorescence spectroscopic detection and measurement of single telomere molecules Journal Article

Nucleic Acids Res, 46 (19), pp. e117, 2018, ISSN: 1362-4962 (Electronic) 0305-1048 (Linking).

Abstract | Links | BibTeX

A. M. Kaushik; K. Hsieh; T. H. Wang

Droplet microfluidics for high-sensitivity and high-throughput detection and screening of disease biomarkers Journal Article

Wiley Interdiscip Rev Nanomed Nanobiotechnol, 10 (6), pp. e1522, 2018, ISSN: 1939-0041 (Electronic) 1939-0041 (Linking).

Abstract | Links | BibTeX

Y. Zhang; L. Chen; K. Hsieh; T. H. Wang

Ratiometric Fluorescence Coding for Multiplex Nucleic Acid Amplification Testing Journal Article

Anal Chem, 90 (20), pp. 12180-12186, 2018, ISSN: 1520-6882 (Electronic) 0003-2700 (Linking).

Abstract | Links | BibTeX

N. Andini; A. Hu; L. Zhou; S. Cogill; T. H. Wang; C. T. Wittwer; S. Yang

A "Culture" Shift: Broad Bacterial Detection, Identification, and Antimicrobial Susceptibility Testing Directly from Whole Blood Journal Article

Clin Chem, 64 (10), pp. 1453-1462, 2018, ISSN: 1530-8561 (Electronic) 0009-9147 (Linking).

Abstract | Links | BibTeX

L. Chen; D. J. Shin; S. Zheng; J. H. Melendez; C. A. Gaydos; T. H. Wang

Direct-qPCR Assay for Coupled Identification and Antimicrobial Susceptibility Testing of Neisseria gonorrhoeae Journal Article

ACS Infect Dis, 4 (9), pp. 1377-1384, 2018, ISSN: 2373-8227 (Electronic) 2373-8227 (Linking).

Abstract | Links | BibTeX

C. M. O'Keefe; T. R. 2nd Pisanic; H. Zec; M. J. Overman; J. G. Herman; T. H. Wang

Facile profiling of molecular heterogeneity by microfluidic digital melt Journal Article

Sci Adv, 4 (9), pp. eaat6459, 2018, ISSN: 2375-2548 (Electronic) 2375-2548 (Linking).

Abstract | Links | BibTeX

K. Hsieh; H. C. Zec; L. Chen; A. M. Kaushik; K. E. Mach; J. C. Liao; T. H. Wang

Simple and Precise Counting of Viable Bacteria by Resazurin-Amplified Picoarray Detection Journal Article

Anal Chem, 90 (15), pp. 9449-9456, 2018, ISSN: 1520-6882 (Electronic) 0003-2700 (Linking).

Abstract | Links | BibTeX

C. Surrette; B. Scherer; A. Corwin; G. Grossmann; A. M. Kaushik; K. Hsieh; P. Zhang; J. C. Liao; P. K. Wong; T. H. Wang; C. M. Puleo

Rapid Microbiology Screening in Pharmaceutical Workflows Journal Article

SLAS Technol, 23 (4), pp. 387-394, 2018, ISSN: 2472-6311 (Electronic) 2472-6303 (Linking).

Abstract | Links | BibTeX

Jian Song; Jennifer Dailey; Hui Li; Hyun-June Jang; Luisa Russell; Pengfei Zhang; Peter C. Searson; Jeff Tza-Huei Wang; Allen D. Everett; Howard E. Katz

Influence of Bioreceptor Layer Structure on Myelin Basic Protein Detection using Organic Field Effect Transistor-Based Biosensors Journal Article

Advanced Functional Materials, 28 (37), pp. 1802605, 2018, ISSN: 1616301X.

Abstract | Links | BibTeX

D. J. Shin; A. Y. Trick; Y. H. Hsieh; D. L. Thomas; T. H. Wang

Sample-to-Answer Droplet Magnetofluidic Platform for Point-of-Care Hepatitis C Viral Load Quantitation Journal Article

Sci Rep, 8 (1), pp. 9793, 2018, ISSN: 2045-2322 (Electronic) 2045-2322 (Linking).

Abstract | Links | BibTeX

D. J. Shin; M. Lewis; Y. H. Hsieh; N. A. Rahmoun; C. A. Gaydos; T. H. Wang; R. Rothman

Healthcare worker feedback on a prototype smartphone-based point-of-care test platform for use in episodic care Journal Article

Point Care, 17 (2), pp. 63-65, 2018, ISSN: 1533-029X (Print) 1533-029X (Linking).

Abstract | Links | BibTeX

A. Stark; D. J. Shin; T. H. Wang

A sample-to-answer droplet magnetofluidic assay platform for quantitative methylation-specific PCR Journal Article

Biomed Microdevices, 20 (2), pp. 31, 2018, ISSN: 1572-8781 (Electronic) 1387-2176 (Linking).

Abstract | Links | BibTeX

Helena C. Zec; Tony Zheng; Lingshu Liu; Kuangwen Hsieh; Tushar D. Rane; Todd Pederson; Tza-Huei Wang

Programmable microfluidic genotyping of plant DNA samples for marker-assisted selection Journal Article

Microsystems & Nanoengineering, 4 , pp. 17097, 2018, ISSN: 2055-7434.

Abstract | Links | BibTeX

2017

A. M. Kaushik; K. Hsieh; L. Chen; D. J. Shin; J. C. Liao; T. H. Wang

Accelerating bacterial growth detection and antimicrobial susceptibility assessment in integrated picoliter droplet platform Journal Article

Biosens Bioelectron, 97 , pp. 260-266, 2017, ISSN: 1873-4235 (Electronic) 0956-5663 (Linking).

Abstract | Links | BibTeX

D. Kilburn; Y. Song; T. H. Wang; K. J. Liu

A Multiplex Ligation Assay for miRNA Copy Number Profiling Journal Article

Methods Mol Biol, 1509 , pp. 185-193, 2017, ISSN: 1940-6029 (Electronic) 1064-3745 (Linking).

Abstract | Links | BibTeX

139 entries « 1 of 4 »

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Patents

  1. H. Wang, F.M. Friedrich, J.M. Burke, and K. Liu “Concentration and Accumulation of Target Species in Response to a Gradient of Solute or Solvent” (JHU Ref. C13791; US provisional patent)
  2. H. Wang, K. Hsieh, H. Zec, L. Liu, A.M. Kaushik, Y. Yun, “Multiplexed, Continuous-flow, droplet-based platform for high-throughput genetic detection” (JHU Ref. C13529; US provisional patent)
  3. H. Wang, T.D. Rane, H.C Zec, “System and device for high throughput generation of combinatorial droplets and methods of use”  (PCT/US2015/012927; JHU Ref. C12862)
  4. H. Wang, D.J. Shin, “Self-contained cartridge and methods for integrated biochemical assay at the point-of-care” (JHU Ref. C12720)
  5. -H. Wang, S. Yang, M.A. Jacobs, P. Athamanolap, S.I. Fraley, V. Agarwal, V. Parekh. “Melt curve classifier for reliable large-scale genotyping of sequence variants” JHU Case Number C12600, provisional patent
  6. Stephanie I. Fraley, T.-H. Wang, and S. Yang. “A novel technology for broad-based, yet single molecule sensitive profiling in heterogeneous biological samples.” JHU Case Number C12403, provisional patent
  7. H. Wang, Y. Zhang, “Fabrication of Hierarchical Silica Nanomembrane using Heat-Shrinking Polymers and Applications of Silica Nanomembrane for the Solid Phase Extraction of Nucleic Acids”, JHU Case Number C12404, provisional patent
  8. H. Wang, Chi-Hang Chiou, Dong Jin Shin , “Electromagnetically Actuated Droplet Microfluidic Chip and System” (JHU Ref. C12262)
  9. H. Wang and Y. Zhang, “Surface Energy Traps (SETs) Enabled Complex Droplet Manipulation” (JHU Ref. C11971)
  10. H. Wang, K. Liu and Y. Song, “miRNA Analysis Method” (US Provisional US 61/598513, JHU Ref. C11885)
  11. H. Wang, T.D. Rane, H. C. Zec and W.Ch. Chu “Systems and methods for screening a library of samples” (US13/708,510. JHU Ref. C11803)
  12. Yang, T.H. Wang, S.K. Park and Y Zhang, “Method and apparatus for continuous microfluidic sample separation and concentration using AC electric field” (JHU Ref. C11562)
  13. H. Wang, Y. Zhang, S. Park and S. Yang, “Self-sustained Droplet Microfluidic Chip and System for Integrated Sample Preparation and Nucleic Acids Detection” (PCT/US2011/045363, JHU Ref. C11183)
  14. H. Wang, H.Q. Mao, W. Beh and D. Kraitchman, “Kilohertz Generation of Monodisperse Microbeads on a Polymeric Membrane Microfluidic Pseudocheck Valve Device” (PCT/US2011/054598, JHU Ref. C11248)
  15. Ahuja, V. Bailey, S.B. Baylin, J.G. Herman, T.H. Wang, J. Yi, “Early Detection of DNA Methylation Biomarker in Cancer Patient Sera” (PCT/US2011/037926, JHU Ref. C11091)
  16. H. Wang and K. Liu, “Hydrodynamic Particle Separation and Detection Systems and Methods”, (PCT/US2011/056941, JHU Ref. C11263)
  17. H. Wang and K.J. Liu, “Single Molecule Spectroscopy for Analysis of Cell-free Nucleic Acid Biomarkers” (PCT/US2010/033888, JHU Ref. C10750)
  18. H. Wang and Y. Zhang, ” Device and Method of Preparing and Performing Multiple Polymerase Chain Reactions” (US 12/716,031, JHU Ref. C10659)
  19. H. Wang, S. Baylin, J. Herman, H. Easwaran and H. Carraway, “Compositions and Methods for Polynucleotide Extraction and Methylation Detection” (PCT/US2009/000039, JHU Ref. C10249)
  20. M. Ho and T.H. Wang, “Biosensors and Methods for Their Use” (PCT/US2001/025444)
  21. H. Wang, K.J. Liu, and I.M. Shih, ” DNA Integrity Assay (DIA) for Cancer Diagnostics, Using Confocal Fluorescence Spectroscopy” (US8,835,110, JHU Ref. C10534)
  22. H. Wang and K. Liu, “Cylindrical Illumination Confocal Spectroscopy System” (US8,248,609, JHU Ref. C10398)
  23. H. Wang, K.J. Liu, C.M. Puleo and T. Rane, ” Microfluidic System for High-Throughput, Droplet-based, Single Molecule Analysis with Low Reagent Consumption” (US9,284,601, issued on 03/15/2016, JHU Ref. C10662)
  24. F. Miller, J. Huang, T.H. Wang, C.M. Ho and M. Liu, “Electrochemical Detection of Mismatch in Nucleic Acids” (US 7,291,457)
  25. H. Wang, “Method for determining standard cycle time of a stage dynamically” (US5,825,650)
  26. A. Hulbert, J. Herman, M. Brock, T.H. Wang, and A. Stark “Early detection of Individuals at High Risk for Lung Cancer of all Stages Using Gene promoter Methylation in Plasma and Sputum” JHU C13599

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Conference Publications

  1. J. Shin, P. Athamanolap, L.Chen, J. Hardick, CA Gaydos and T.H. Wang, “A Smartphone-Based Mobile NAAT Diagnostic Suite for Chlamydia Detection”, Proc. 26th Anniversary World Congress on Biosensors (Biosensors 2016), (accepted)
  2. M. Friedrich, J.M. Burke, K.J. Liu, T.H. Wang, “In-line Preconcentration, Size Separation, and Single-Molecule Detection Without Applied Electric Fields, Proc. 29th IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2016), p. 181-184, 2016
  3. J. Shin, L. Chen and T.H. Wang, “Single-bacteria Confocal Spectroscopy: An Ultrasensitive method for Real-time Monitoring of Bacterial Growth”, Proc. 19th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2015), p. 340-342, 2015
  4. Kaushik, K. Hsieh, L. Chen, D.J. Shin and T.H. Wang, “Rapid Assessment of Bacterial Vitality and Antibiotic Susceptibility via High-Throughput Picoliter-Droplet Single-Cell Assay”, Proc. 19th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2015), p. 531-533, 2015
  5. Liu, K. Hsieh, A. Kaushik, H.C. Zec and T.H. Wang, “Multiplexed, Continuous-Flow, Droplet-Based PCR Genotyping Platform for High-Throughput Agriculture Marker Assisted Selection”, Proc. 19th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2015), p. 1368-1370, 2015
  6. Zec, C. O’Keefe, P. Ma, T.H. Wang, “Ultra-Thin, Evaporation-Resistant PDMS Devices for Absolute Quantification of DNA Using Digital PCR”, The 18th International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers 2015), p.536-539, 2015
  7. Guan, L. Chen, T. Rane, A. Kaushik, T.H. Wang, “Digital Droplet ELOHA For Nucleic Molecule Counting And Analysis”, The 18th International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers 2015), p.536-5460-463, 2015
  8. C. Zec, C.J. Glover, W. Hsieh, L. Liu, C. O’Keefe and T.H. Wang, “Methods for Controlling Water Evaporation in PDMS-Based Microfluidic Devices”, Proc. 18th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2014), p. 1743-1745, 2014
  9. J. Shin, P. Athamanonolap, L. Chen and T.H. Wang, “Integrated droplet microfluidic platform for nucleic acids amplification test of Chlamydia trachomatis infection”, Proc. 24th Anniversary World Congress on Biosensors (Biosensors 2014)
  10. C. Zec, T.D. Rane, P. Ma and T.H. Wang, “Parallelization of Fission and Fusion- Operations for High Throughput Generation of Combinatorial Droplets”, Proc. 27th IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2014), p. 334-337, 2014
  11. J. Shin, L. Chen and T.H. Wang, “A Simple Integrated Diagnostic Platform for DNA Testing of Chlamydia Trachomatis Infection, “ Proc. 17th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2013), p. 1350-1352, 2013
  12. M. Friedrich, K.J. Liu and T.H. Wang, “ Single Molecule Hydrodynamic Separation for Ultrasensitive and Quantitative DNA Size Separations,” Proc. 17th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2013), p. 35-37, 2013
  13. D. Rane, H.C. Zec and T.H. Wang, “A Multiplexed Microfluidic Droplet Platform for Matrix Metalloproteinase Screening”, Proc. 17th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2013), p. 1595-1597, 2013
  14. K. Wu, S. F. Friedrich, K.J. Liu and T.H. Wang, “ Chip-Based DNA Separation in Free Solution by Inertial Hydrodynamic Forces”, Proc. 17th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2013), p. 578-580, 2013
  15. J. Shin, A. Stark and T.H. Wang, “ Droplet Bisulfite Conversion Platform for Epigenetic Cancer Biomarker Detection”, The 17th International Conference on Solid-State Sensors, Actuators and Microsystems(Transducers 2013), p.2181-2184, 2013
  16. Zhang, Y. Zhang, B. Keeley, A. Stark and T.H. Wang, “Spontaneous Shrinking Silica Nanomembrane for Solid Phase”, Proc. 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (IEEE NEMS 2013), p. 444-445, 2013
  17. Athamanolap, B. Keeley, D.J. Shin and T.H. Wang, “Quantitative Analysis of DNA Methylation Based on Melting Curve Analysis”, Proc. 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (IEEE NEMS 2013), p.1116-1118, 2013
  18. Zhang, Y Zhang, T.H. Wang, “Hierarchical Silica Nanomembrane Driven by Thermal Shrinkage and its Application for Solid Phase DNA Extraction”, Proc. The 13th IEEE International Conference on Nanotechnology (IEEE NANO 2013), 2013
  19. C. Zec, T.D. Rane, W.C. Chu, V. Wang and T.H. Wang, “ A Microfluidic Droplet Platform for Multiplexed Single Nucleotide Polymorphism Analysis of an Array Plant Genomic DNA Samples”, Proc. 26th IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2013), p.263-266, 2013
  20. H. Chiou, D.J. Shin, S. Hosmane, Y Zhang and T.H. Wang, “Electromagnet-Actuated Droplet Platform for Sample-to-Answer Genetic Detection”, Proc. 26th IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2013), p.98-101, 2013
  21. Zhang and T.H. Wang, “ All-in-One Droplet Platform for Multiplexed Genetic Detection in Blood” Proc. 26th IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2013), p. 1061-1064, 2013
  22. Zhang and T.H. Wang, “Flip-Drop: Droplet Array Created by Surface Energy Trap for Combinatorial Screening,” Proc. 26th IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2013), p.1149-1152, 2013
  23. W. Beh, C. Weiss, H.Q. Mao, D. L. Kraitchman, and T.H. Wang, “High-Throughput Microfluidic Preparation of Imaging-Visible Embolic Beads”, Proc. EMBS Micro and Nanotechnology in Medicine Conference, p.59, 2012
  24. Zhang, Y. Zhang, B. Keeley, A. Stark and T.H. Wang, “Fabricating and Applying Hierarchical Silica Nanomembrane with for Solid Phase DNA Extraction” The 6th IEEE International Conference on Nano/Molecular Medicine and Engineering (IEEE NANOMED 2012), 2012
  25. Keeley, Yi Zhang, Ye Zhang, A. Stark, T.H. Wang, “Quantum Dot FRET Linker Probes for Highly Sensitive DNA Methylation Detection”, Proc. IEEE 12th International Conference on Nanotechnology (IEEE NANO 2012), 7848, p.1-4, 2012
  26. Zhang and T.H. Wang, “Quantum Dot Electrophoretic Mobility Shift Assay and Its Application to the Measurement of Exonuclease Activity” Proc. IEEE 12th International Conference on Nanotechnology (IEEE NANO 2012), 7685, p.1-4, 2012
  27. Song, L. Zhang, M. Chen and T.H. Wang, “Single Quantum Dot Fluorescence Enhancement by Tunable Nanoporous Gold”, Proc. IEEE 12th International Conference on Nanotechnology (IEEE NANO 2012), 7877, p.1-4, 2012
  28. Zhang and T.H. Wang, “Droplet Immobilization, Splitting, Metering and Aliquoting with Surface Energy Traps Created Using SU8 Shadow Mask” Proc. 16th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2012), p. 73-75, 2012
  29. Zhang and T.H. Wang, “Surface Energy Trap Assisted Rapid Serial Dilution on Droplet Platform for Bacteria Antibiotics Susceptibility Test” Proc. 16th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2012), p. 467-469, 2012
  30. J. Liu, T.D. Rane, Y. Zhang, C. Beh, D.J. Shin, T.H. Wang, “An Integrated Platform for Single Molecule Free Solution Hydrodynamic Separation Using Yoctomoles of DNA and Picoliter Samples”, ASME 10th International Conference on Nanochannels, Microchannels and Minichannels (ICNMM 2012), ICNMM2012-73154 (p.1-6), 2012
  31. Zec, T.D. Rane, W.C. Chu and T.H. Wang, “Multiplexed Screening of a Large Library of Biological Samples through on-Demand Droplet Generation and Fusion”, ASME 2012 10th International Conference on Nanochannels, Microchannels and Minichannels (ICNMM 2012), ICNMM2012-73159 (p.1-6), 2012
  32. Song, Y. Zhang, and T.H. Wang, “Single Quantum Dot-Based Multiplexed Point Mutation Detection by Gap Ligase Chain Reaction”, Proc. 15th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2011), p.1779-1781, 2011
  33. Zhang, D.J. Shin and T.H. Wang, “Detecting Genetic Variations in A Droplet”, Proc. 15th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2011), p.1179-1181, 2011
  34. J. Liu and T.H. Wang, “ PCR-free, microfluidic single molecule analysis of circulating nucleic acids in lung cancer patient serum”, Proc. 33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC ’11), p.8392-8395, 2011
  35. Zhang and T.H. Wang, “ An Active Gyroscopic Magnetic Micromixer for Rapid Fluid Mixing in Droplet Based Microfluidic Systems”, The 16th International Conference on Solid-State Sensors, Actuators and Microsystems(Transducers 2011), p.1769-1772, 2011
  36. Zhang, S.K. Park, S. Yang and T.H. Wang, “Fully Integrated Droplet Based Point-of-Care Platform for Molecular Detection from Crude Biosamples”, The 16th International Conference on Solid-State Sensors, Actuators and Microsystems(Transducers 2011), p.1927-1930, 2011
  37. H. Wang, V. Bailey and K. Liu, “Quantum Dots and Microfluidic Single Molecule Detection for Screening Genetic and Epigenetic Cancer Markers in Clinical Samples”, Proc. 2011 SPIE Defense, Security and Sensing Conference, Volume 8031, P. 80311W
  38. D. Rane, H. Zec, C.M. Puleo, A.P. Lee and T.H. Wang, “High-Throughput Single-Cell Pathogen Detection on a Droplet Microfluidic Platform”, Proc. 24th IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2011), p.881-884, 2011
  39. Stark, Y. Zhang, V. Bailey, B. Keeley. T.H. Wang, “Increasing Throughput and Sensitivity of DNA Methylation Analysis through Functional Nanoparticles” The 6th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (IEEE NEMS 2011), 1091-1094, 2011
  40. Y Zhang and T.H. Wang, “A Quantum Dot Based Electrophoretic Mobility Shift Assay for High Resolution Copy Number Variation Study”, The 6th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (IEEE NEMS 2011), p.841-844, 2011
  41. Zhang, I.M. Shih, T.L. Wang and T.H. Wang, ” A Quantum Dot Based Nanoassay for Quantifying Gene Copy Number with Ultrahigh Resolution”, Proc. 14th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2010), p.1154-1156, 2010
  42. W. Beh, W. Zhou and T.H. Wang, “Oxygen Plasma-Free Microfluidic Device Sealing”, Proc. 14th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2010) p.1217-1219, 2010
  43. W. Beh, D. Kraitchman, H.Q. Mao, T.H. Wang, “High-throughput Monodisperse Alginate Gel Bead Formation using Microfluidic Pseudo-Check Valve”, Proc. 14th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2010) p.425-427, 2010
  44. Zhang and T.H. Wang, “An Automated All-in-one Microfluidic Device for Parallel Solid Phase DNA Extraction and Droplet-in-Oil PCR Analysis”, Proc. 23rd IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2010), P.971-974, 2010
  45. Zhang and T.H. Wang, “Geomorphology-Assisted Manopulation of Magnet-Actuated Droplet for Solid Phase DNA Extraction and Droplet-in-Oil PCR”, Proc. 23rd IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2010), p.1047-1050, 2010
  46. D. Rane, C.M. Puleo, H. Zec, Y. Zhang, A.P. Lee and T.H. Wamh, “Analyte Detection in Droplets: One Molecule at a Time”, Proc. 13th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2009), p.15-17, 2009
  47. M. Puleo, H.C. Zec, Y. Sung and T.H. Wang, “Micro-evaporator as Interconnects to Low-Volume Microfluidic Components”, Proc. 13th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2009), p.932-935, 2009
  48. J. Bailey, C.M. Puleo, Y.P. Ho, H.C. Yeh, T.H. Wang, “Quantum Dots in Molecular Detection of Diseases”, 31st Annual International Conference on the IEEE Engineering in Medicine and Biology Society ( IEEE EMBC 2009), p. 4089-4092, 2009
  49. Zhang, V. Bailey, C.M. Puleo, H. Easwaran, E. Griffiths, J.G. Herman, S.B. Baylin, T.H. Wang, “High Throughput DNA Methylation Analysis on a Droplet-in-Oil Polymerase Chain Reaction Array”, The 15th International Conference on Solid-State Sensors, Actuators and Microsystems(Transducers 2009), p.806-808, 2009
  50. Zhang, V. Bailey, C.M. Puleo, C. Chen and T.H. Wang, “Multiple gene Analysis within a Simple Droplet-in-Oil Microfluidic PCR Platform”, Proc. 12th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2008), p. 751-753 , 2008
  51. P. Ho, H.H. Chen, K. Leong and T.H. Wang “Quantitative Kinetic Analysis of DNA Nanocomplex Self-Assembly with Quantum Dots FRET in a Microfluidic Device”, Proc. 21st IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2008), p. 30-33, 2008
  52. M. Puleo, H.C. Yeh and T.H. Wang “Single Molecule Detection in Truly, Nanoliter-Sized Volumes: Coupling Evaporation-Based, Microfluidic Concentration with Confocal Fluorescence Spectroscopy” Proc. 21st IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2008), p.200-203, 2008
  53. Liu and T.H. Wang, “Quantitative Confocal Spectroscopy – Rectifying the Limitations of Single Molecule Detection”, Proc. 3rd Annual IEEE International Conferences on Nano/Micro Engineered and Molecular Systems (IEEE-NEMS 2008), p. 1189-1192, 2008
  54. C. Yeh. Y.P. Ho, C.M. Puleo and T.H. Wang,” Towards single-molecule diagnostics using microfluidic manipulation and quantum dot nanosensors, Proc. 5th International Conference on Nanochannels, Microchannels and Minichannels, (ICNMM 2007), p. 1133-1140, 2007
  55. P. Ho and T.H. Wang, “Multiplexed Detection of Anthrax Sequences with Quantum Dot Nanoprobes”, Proc. IEEE/NLM Life Science Systems and Application Workshop, p. 62-63, 2006
  56. Murray , K. Rebello, J. Crookston, J. Miragliotta and T.H. Wang, “High-degree Concentration of Bio-agents Using Electrokinetic Manipulations”, Proc. IEEE/NLM Life Science Systems and Application Workshop, p. 94-95, 2006
  57. P. Ho, M.C. Kung and T.H. Wang, ” Separation-free Detection of Low-abundant Biomolecules with Two-Color Colocalization of Quantum Dot Probes,” Proc. 9th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2005), p. 1330-1332, 2005
  58. C. Yeh, Y.P. Ho and T.H. Wang, “Quantum Dot-Mediated Separation-Free Assay for Point Mutation Detection ” Proc. NSTI- Bio-Nanotechnology Conference, 198-201, 2005
  59. C. Yeh, E. Simone, C.Y. Zhang and T.H. Wang, “Single Bio-Molecule Detection with Quantum-dots in Flow-rate Controlled Microchannel”, Proc. 17h IEEE Annual Workshop of Micro Electro Mechanical Systems (IEEE MEMS 2004), p 371-374, 2004
  60. Y. Chao, C.Y. Zhang and T.H. Wang,” Measurement of in-situ Flow Velocity Using Single-Molecule Detection for the Application of Biomolecule Quantification”, Proc. Hilton Head 2004 Solid-State Sensor, Actuator, and Microsystems Workshop (Hilton Head 2004), p. 176-179, 2004
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  64. K. Wong, T.H. Wang and C.M. Ho, “Optical Fiber Tip Fabricated by Surface Tension Controlled Etching”, Proc. Hilton Head 2002: Solid-State Sensor, Actuator, and Microsystems Workshop (Hilton Head 2002), p. 94-97, 2002
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