Journal Publications | Patents | Conference Publications

Journal Publications

186 entries « 1 of 10 »
1.

Ngo, HT.; Akarapipad, P.; Lee, PW.; Park, JS.; Chen, FE.; Trick, AY.; Hsieh, K.; Wang, TH.

Rapid and Portable Quantification of HIV RNA via a Smartphone-enabled Digital CRISPR Device and Deep Learning Online

2023, visited: 16.05.2023.

Abstract | Links | BibTeX

2.

Tjandra, KC.; Ram-Mohan, N.; Abe, R.; Wang, TH.; Yang, S.

Rapid Molecular Phenotypic Antimicrobial Susceptibility Test for Neisseria gonorrhoeae Based on Propidium Monoazide Viability PCR Journal Article

In: ACS Infectious Diseases, vol. 9, iss. 5, pp. 1160-1167, 2023.

Abstract | Links | BibTeX

3.

Chen, F.; Wang, J.; Nambiar, AH.; Hardick, J.; Melendez, J.; Trick, AY.; Wang, TH.

Point-of-Care Amenable Detection of Mycoplasma genitalium and Its Antibiotic Resistance Mutations Journal Article

In: ACS Sensors, vol. 8, iss. 4, pp. 1550-1557, 2023.

Abstract | Links | BibTeX

4.

Zhao, Y.; O'Keefe, CM.; Hsieh, K.; Cope, L.; Joyce, SC.; Pisanic, TR.; Herman, JG.; Wang, TH.

Multiplex Digital Methylation‐Specific PCR for Noninvasive Screening of Lung Cancer Journal Article

In: Advanced Science, vol. e2206518, 2023.

Abstract | Links | BibTeX

5.

Zhu, X.; Sakamoto, S.; Ishii, C.; Smith, MD.; Ito, K.; Obayashi, M.; Unger, L.; Hasegawa, Y.; Kurokawa, S.; Kishimoto, T.; Li, H.; Hatano, S.; Wang, TH.; Yoshikai, Y.; Kano, S.; Fukuda, S.; Sanada, K.; Calabresi, PA.; Kamiya, A.

Dectin-1 signaling on colonic γδ T cells promotes psychosocial stress responses Journal Article

In: Nature Immunology, vol. 24, pp. 625-636, 2023.

Abstract | Links | BibTeX

6.

Shao, F.; Park, JS.; G. Zhao,; Hsieh, K.; Wang, TH.

Elucidating the Role of CRISPR/Cas in Single-Step Isothermal Nucle-ic Acid Amplification Testing Assays Journal Article

In: Analytical Chemistry, vol. 95, iss. 7, pp. 3873-3882, 2023.

Abstract | Links | BibTeX

7.

Li, H.; Hsieh, K.; Wong, P.; Mach, K.; Liao, J.; Wang, TH.

Single-cell pathogen diagnostics for combating antibiotic resistance Journal Article

In: Nature Reviews Methods Primers, vol. 3, iss. 1, no. 6, 2023.

Links | BibTeX

8.

Ngo, HT.; Jin, M.; Trick, AY.; Chen, FE.; Chen, L.; Hsieh, K.; Wang, TH.

Sensitive and Quantitative Point-of-Care HIV Viral Load Quantification from Blood Using a Power-Free Plasma Separation and Portable Magnetofluidic Polymerase Chain Reaction Instrument Journal Article

In: Analytical Chemistry, vol. 95, iss. 2, pp. 1159–1168, 2023.

Abstract | Links | BibTeX

9.

Akarapipad, P.; Bertelson, E.; Pessell, A.; Wang, TH.; Hsieh, K.

Emerging Multiplex Nucleic Acid Diagnostic Tests for Combating COVID-19 Journal Article

In: Biosensors, vol. 12, iss. 11, pp. 978, 2022.

Abstract | Links | BibTeX

10.

Li, S.; Hu, Y.; Li, A.; Lin, J.; Hsieh, K.; Schneiderman, Z.; Zhang, P.; Zhu, Y.; Qiu, C.; Kokkoli, E.; Wang, TH.; Mao, HQ.

Payload distribution and capacity of mRNA lipid nanoparticles Journal Article

In: nature communications, vol. 13, no. 5561, 2022.

Abstract | Links | BibTeX

11.

Zhang, P.; Hu, J.; Park, JS.; Hsieh, K.; Chen, L.; Mao, A.; Wang, TH.

Highly Sensitive Serum Protein Analysis Using Magnetic Bead-Based Proximity Extension Assay Journal Article

In: Analytical Chemistry, vol. 94, iss. 36, pp. 1281-12489, 2022.

Abstract | Links | BibTeX

12.

Shao, F.; Hsieh, K.; Zhang, P.; Kaushik, AM.; Wang, TH.

Facile and scalable tubing-free sample loading for droplet microfluidics Journal Article

In: Scientific Reports, vol. 12, iss. 1, no. 13340, pp. 1-12, 2022.

Abstract | Links | BibTeX

13.

Ma, K.; Kalra, A.; Tsai, HL.; Okello, S.; Cheng, Y.; Meltzer, SJ.; Consortium, Esophagea Squamous Cell Carcinoma

Accurate Nonendoscopic Detection of Esophageal Squamous Cell Carcinoma Using Methylated DNA Biomarkers Journal Article

In: Gastroenterology, vol. 163, iss. 2, pp. 507-509, 2022.

Abstract | Links | BibTeX

14.

Shao, F.; Lee, P.; Li, H.; Hsieh, K.; Wang, TH.

Emerging platforms for high-throughput enzymatic bioassays Journal Article

In: Trends in Biotechnology, vol. 41, iss. 1, pp. 120-133, 2022.

Abstract | Links | BibTeX

15.

Chen, FE.; Trick, AY.; Hasnain, AC.; Hsieh, K.; Chen, L.; Shin, DJ.; Wang, TH.

Ratiometric PCR in a Portable Sample-to-Result Device for Broad-Based Pathogen Identification Journal Article

In: Analytical Chemistry, vol. 94, iss. 26, pp. 9372-9379, 2022.

Abstract | Links | BibTeX

16.

Liberto, JM.; Chen, SY.; Shih, IM.; Wang, TH.; Wang, TL.; Pisanic, TR.

Current and Emerging Methods for Ovarian Cancer Screening and Diagnostics: A Comprehensive Review Journal Article

In: Cancers, vol. 14, iss. 12, no. 2885, 2022.

Abstract | Links | BibTeX

17.

Stark, A.; II, TR. Pisanic; Herman, JG.; Wang, TH.

High-throughput sample processing for methylation analysis in an automated, enclosed environment Journal Article

In: SLAS Technology, vol. 27, iss. 3, pp. 172-179, 2022.

Abstract | Links | BibTeX

18.

Hamill, MM.; Onzia, A.; Wang, TH.; Kiragga, AN.; Hsieh, YH.; Parkes-Ratanshi, R.; Gough, E.; Kyambadde, P.; Melendez, JH.; Manabe, YC.

High burden of untreated syphilis, drug resistant Neisseria gonorrhoeae, and other sexually transmitted infections in men with urethral discharge syndrome in Kampala, Uganda Journal Article

In: BMC Infectious Diseases, vol. 22, iss. 1, pp. 1-9, 2022.

Abstract | Links | BibTeX

19.

Lee, PW.; Totten, M.; Chen, L.; Chen, FE.; Trick, AY.; Shah, K.; Ngo, HT.; Jin, M.; Hsieh, K.; Zhang, SX.; Wang, TH.

A portable droplet magnetofluidic device for point-of-care detection of multidrug-resistant Candida auris Journal Article

In: Frontiers in Bioengineering and Biotechnology, vol. 10, no. 926694, 2022.

Abstract | Links | BibTeX

20.

Trick, A. Y.; Ngo, H. T.; Nambiar, AH.; Morakis, MM.; Chen, FE.; Chen, L.; Hsieh, K.; Wang, T. H.

Filtration-assisted magnetofluidic cartridge platform for HIV RNA detection from blood Journal Article

In: Lab on a Chip, vol. 22, pp. 945-953, 2022.

Abstract | Links | BibTeX

186 entries « 1 of 10 »

186 entries « 1 of 5 »

2023

HT. Ngo; P. Akarapipad; PW. Lee; JS. Park; FE. Chen; AY. Trick; K. Hsieh; TH. Wang

Rapid and Portable Quantification of HIV RNA via a Smartphone-enabled Digital CRISPR Device and Deep Learning Online

2023, visited: 16.05.2023.

Abstract | Links | BibTeX

KC. Tjandra; N. Ram-Mohan; R. Abe; TH. Wang; S. Yang

Rapid Molecular Phenotypic Antimicrobial Susceptibility Test for Neisseria gonorrhoeae Based on Propidium Monoazide Viability PCR Journal Article

In: ACS Infectious Diseases, vol. 9, iss. 5, pp. 1160-1167, 2023.

Abstract | Links | BibTeX

F. Chen; J. Wang; AH. Nambiar; J. Hardick; J. Melendez; AY. Trick; TH. Wang

Point-of-Care Amenable Detection of Mycoplasma genitalium and Its Antibiotic Resistance Mutations Journal Article

In: ACS Sensors, vol. 8, iss. 4, pp. 1550-1557, 2023.

Abstract | Links | BibTeX

Y. Zhao; CM. O'Keefe; K. Hsieh; L. Cope; SC. Joyce; TR. Pisanic; JG. Herman; TH. Wang

Multiplex Digital Methylation‐Specific PCR for Noninvasive Screening of Lung Cancer Journal Article

In: Advanced Science, vol. e2206518, 2023.

Abstract | Links | BibTeX

X. Zhu; S. Sakamoto; C. Ishii; MD. Smith; K. Ito; M. Obayashi; L. Unger; Y. Hasegawa; S. Kurokawa; T. Kishimoto; H. Li; S. Hatano; TH. Wang; Y. Yoshikai; S. Kano; S. Fukuda; K. Sanada; PA. Calabresi; A. Kamiya

Dectin-1 signaling on colonic γδ T cells promotes psychosocial stress responses Journal Article

In: Nature Immunology, vol. 24, pp. 625-636, 2023.

Abstract | Links | BibTeX

F. Shao; JS. Park; G. Zhao,; K. Hsieh; TH. Wang

Elucidating the Role of CRISPR/Cas in Single-Step Isothermal Nucle-ic Acid Amplification Testing Assays Journal Article

In: Analytical Chemistry, vol. 95, iss. 7, pp. 3873-3882, 2023.

Abstract | Links | BibTeX

H. Li; K. Hsieh; P. Wong; K. Mach; J. Liao; TH. Wang

Single-cell pathogen diagnostics for combating antibiotic resistance Journal Article

In: Nature Reviews Methods Primers, vol. 3, iss. 1, no. 6, 2023.

Links | BibTeX

HT. Ngo; M. Jin; AY. Trick; FE. Chen; L. Chen; K. Hsieh; TH. Wang

Sensitive and Quantitative Point-of-Care HIV Viral Load Quantification from Blood Using a Power-Free Plasma Separation and Portable Magnetofluidic Polymerase Chain Reaction Instrument Journal Article

In: Analytical Chemistry, vol. 95, iss. 2, pp. 1159–1168, 2023.

Abstract | Links | BibTeX

2022

P. Akarapipad; E. Bertelson; A. Pessell; TH. Wang; K. Hsieh

Emerging Multiplex Nucleic Acid Diagnostic Tests for Combating COVID-19 Journal Article

In: Biosensors, vol. 12, iss. 11, pp. 978, 2022.

Abstract | Links | BibTeX

S. Li; Y. Hu; A. Li; J. Lin; K. Hsieh; Z. Schneiderman; P. Zhang; Y. Zhu; C. Qiu; E. Kokkoli; TH. Wang; HQ. Mao

Payload distribution and capacity of mRNA lipid nanoparticles Journal Article

In: nature communications, vol. 13, no. 5561, 2022.

Abstract | Links | BibTeX

P. Zhang; J. Hu; JS. Park; K. Hsieh; L. Chen; A. Mao; TH. Wang

Highly Sensitive Serum Protein Analysis Using Magnetic Bead-Based Proximity Extension Assay Journal Article

In: Analytical Chemistry, vol. 94, iss. 36, pp. 1281-12489, 2022.

Abstract | Links | BibTeX

F. Shao; K. Hsieh; P. Zhang; AM. Kaushik; TH. Wang

Facile and scalable tubing-free sample loading for droplet microfluidics Journal Article

In: Scientific Reports, vol. 12, iss. 1, no. 13340, pp. 1-12, 2022.

Abstract | Links | BibTeX

K. Ma; A. Kalra; HL. Tsai; S. Okello; Y. Cheng; SJ. Meltzer; Esophagea Squamous Cell Carcinoma Consortium

Accurate Nonendoscopic Detection of Esophageal Squamous Cell Carcinoma Using Methylated DNA Biomarkers Journal Article

In: Gastroenterology, vol. 163, iss. 2, pp. 507-509, 2022.

Abstract | Links | BibTeX

F. Shao; P. Lee; H. Li; K. Hsieh; TH. Wang

Emerging platforms for high-throughput enzymatic bioassays Journal Article

In: Trends in Biotechnology, vol. 41, iss. 1, pp. 120-133, 2022.

Abstract | Links | BibTeX

FE. Chen; AY. Trick; AC. Hasnain; K. Hsieh; L. Chen; DJ. Shin; TH. Wang

Ratiometric PCR in a Portable Sample-to-Result Device for Broad-Based Pathogen Identification Journal Article

In: Analytical Chemistry, vol. 94, iss. 26, pp. 9372-9379, 2022.

Abstract | Links | BibTeX

JM. Liberto; SY. Chen; IM. Shih; TH. Wang; TL. Wang; TR. Pisanic

Current and Emerging Methods for Ovarian Cancer Screening and Diagnostics: A Comprehensive Review Journal Article

In: Cancers, vol. 14, iss. 12, no. 2885, 2022.

Abstract | Links | BibTeX

A. Stark; TR. Pisanic II; JG. Herman; TH. Wang

High-throughput sample processing for methylation analysis in an automated, enclosed environment Journal Article

In: SLAS Technology, vol. 27, iss. 3, pp. 172-179, 2022.

Abstract | Links | BibTeX

MM. Hamill; A. Onzia; TH. Wang; AN. Kiragga; YH. Hsieh; R. Parkes-Ratanshi; E. Gough; P. Kyambadde; JH. Melendez; YC. Manabe

High burden of untreated syphilis, drug resistant Neisseria gonorrhoeae, and other sexually transmitted infections in men with urethral discharge syndrome in Kampala, Uganda Journal Article

In: BMC Infectious Diseases, vol. 22, iss. 1, pp. 1-9, 2022.

Abstract | Links | BibTeX

PW. Lee; M. Totten; L. Chen; FE. Chen; AY. Trick; K. Shah; HT. Ngo; M. Jin; K. Hsieh; SX. Zhang; TH. Wang

A portable droplet magnetofluidic device for point-of-care detection of multidrug-resistant Candida auris Journal Article

In: Frontiers in Bioengineering and Biotechnology, vol. 10, no. 926694, 2022.

Abstract | Links | BibTeX

A.Y. Trick; H.T. Ngo; AH. Nambiar; MM. Morakis; FE. Chen; L. Chen; K. Hsieh; T.H. Wang

Filtration-assisted magnetofluidic cartridge platform for HIV RNA detection from blood Journal Article

In: Lab on a Chip, vol. 22, pp. 945-953, 2022.

Abstract | Links | BibTeX

K. Hsieh; KE. Mach; P. Zhang; JC. Liao; TH. Wang

Combating Antimicrobial Resistance via Single-Cell Diagnostic Technologies Powered by Droplet Microfluidics Journal Article

In: Accounts of Chemical Research, vol. 55, pp. 123–133, 2022.

Abstract | Links | BibTeX

H. Li; P. Zhang; K. Hsieh; T.H. Wang

Combinatorial nanodroplet platform for screening antibiotic combinations Journal Article

In: Lab on a Chip, vol. 22, pp. 621-631, 2022.

Abstract | Links | BibTeX

AY. Trick; FE. Chen; L. Chen; PW. Lee; AC. Hasnain; HH. Mostafa; KC. Carroll; TH. Wang

Point-of-Care Platform for Rapid Multiplexed Detection of SARS-CoV-2 Variants and Respiratory Pathogens Journal Article

In: Advanced Materials Technologies, vol. 7, iss. 6, no. 2101013, 2022.

Abstract | Links | BibTeX

2021

RAM. Klein Kranenbarg; AH. Vali; JNM. IJzermans; TR. Pisanic; TH. Wang; N. Azad; S. Sukumar; M. Fackler

High performance methylated DNA markers for detection of colon adenocarcinoma Journal Article

In: Clinical Epigenetics, vol. 13, iss. 1, pp. 1-15, 2021.

Abstract | Links | BibTeX

P. Zhang; A. Kaushik; K. Hsieh; S. Li; S. Lewis; K.E. Mach; .C. Liao; K.C. Carroll

A Cascaded Droplet Microfluidic Platform Enables High-throughput Single Cell Antibiotic Susceptibility Testing at Scale Journal Article

In: Small Methods, vol. 6, iss. 1, 2021.

Abstract | Links | BibTeX

K. Hsieh,; JH. Melendez; CA. Gaydos; TH. Wang

Bridging the gap between development of point-of-care nucleic acid testing and patient care for sexually transmitted infections Journal Article

In: Lab on a Chip, vol. 22, iss. 3, pp. 476-511, 2021.

Abstract | Links | BibTeX

JH.Melendez; FE. Chen; TH. Wang

Antimicrobial Susceptibility Testing of Neisseria gonorrhoeae using a Phenotypic-Molecular Assay and Lyophilized Antimicrobials Journal Article

In: Diagnostic Microbiology and Infectious Disease, vol. 115590, 2021.

Abstract | Links | BibTeX

L. Ying; A. Sharma; A. Chhoda; N. Ruzgar; N. Hasan; R. Kwak; CL. Wolfgang; TH. Wang; JW. Kunstman; RR. Salem; LD. Wood; C. Iacobuzio-Donahue; EB. Schneider; JJ. Farrell; N. Ahuja

Methylation-based Cell-free DNA Signature for Early Detection of Pancreatic Cancer Journal Article

In: Pancreas , vol. 50, iss. 9, pp. 1267-1273, 2021.

Abstract | Links | BibTeX

A.Y. Trick; F.E. Chen; J.A. Schares; B.E. Freml; P. Lor; Y. Yun; T.H. Wang

High resolution estimates of relative gene abundance with quantitative ratiometric regression PCR (qRR-PCR) Journal Article

In: Analyst, vol. 146, pp. 6463-6469, 2021.

Links | BibTeX

P. Zhang; L. Chen; J. Hu; A.Y. Trick; F.E. Chen; K. Hsieh; Y. Zhao; B. Coleman; K. Kruczynski; T.R Pisanic II; C. D Heaney; W.A, Clarke; Tza-Huei Wang

Magnetofluidic immuno-PCR for point-of-care COVID-19 serological testing Journal Article

In: Biosensors and Bioelectronic, vol. 195, no. 113656, 2021.

Links | BibTeX

B. Forsyth; P. Torab; J.H. Lee; T. Malcom; T.H. Wang; J.C. Liao; S.Yang; E. Kvam; C. Puleo; P.K. Wong

A Rapid Single-Cell Antimicrobial Susceptibility Testing Workflow for Bloodstream Infections Journal Article

In: Biosensors, vol. 11, no. 8, pp. 288, 2021.

Links | BibTeX

L. Chen; K. Wen; F.E. Chen; A.Y. Trick; H. Liu; S. Shao; W. Yu; K. Hsieh; Z. Wang; J. Shen; T.H. Wang

Portable Magnetofluidic Device for Point-of-Need Detection of African Swine Fever Journal Article

In: Analytical Chemistry, vol. 93, no. 31, pp. 10940-10946, 2021.

Links | BibTeX

F.E. Chen; P.W. Lee; A.Y. Trick; J.S. Park; L. Chen; K. Shah; H. Mostafa; K.C. Carroll; K. Hsieh; T.H. Wang

Point-of-Care CRISPR-Cas-Assisted SARS-CoV-2 Detection in an Automated and Portable Droplet Magnetofluidic Device Journal Article

In: Biosensors and Bioelectronics, vol. 113390, 2021.

Abstract | Links | BibTeX

M. Baretti; E. Karunasena; M. Zahurak; R. Walker; Y. Zhao; T.R. Pisanic 2nd; T.H. Wang; T.F. Greten; A.G. Duffy; E. Gootjes; G. Meijer; HMW Verheul; N. Ahuja; J.G. Herman; N.S. Azad

A phase 2 trial of gemcitabine and docetaxel in patients with metastatic colorectal adenocarcinoma with methylated checkpoint with forkhead and ring finger domain promoter and/or microsatellite instability phenotype Journal Article

In: Clinical and Translational Science, vol. 14, pp. 954-963, 2021.

Links | BibTeX

Alexander Y. Trick; Johan H. Melendez; Fan-En Chen; Liben Chen; Annet Onzia; Aidah Zawedde; Edith Nakku-Joloba; Peter Kyambadde; Emmanuel Mande; Joshua Matovu; Maxine Atuheirwe; Richard Kwizera; Elizabeth A. Gilliams; Yu-Hsiang Hsieh; Charlotte A. Gaydos; Yukari C. Manabe; Matthew M. Hamill; Tza-Huei Wang

A portable magnetofluidic platform for detecting sexually transmitted infections and antimicrobial susceptibility Journal Article

In: SCIENCE TRANSLATIONAL MEDICINE, vol. 13, no. 593, pp. eab6356, 2021.

Abstract | Links | BibTeX

P. Zhang; A. Kaushik; K.E. Mach; K. Hsieh; J.C. Liao; T.H, Wang

Facile syringe filter-enabled bacteria separation, enrichment, and buffer exchange for clinical isolation-free digital detection and characterization of bacterial pathogens in urine Journal Article

In: Analyst, 2021.

Links | BibTeX

A.M. Kaushik; K. Hsieh; K.E. Mach; S. Lewis; C.M. Puleo; K.C. Carroll; J.C. Liao; T.H. Wang

Droplet-based single-cell measurements of 16S rRNA enable integrated bacteria identification and pheno-molecular antimicrobial susceptibility testing from clinical samples in 30 min Journal Article

In: Advanced Science, vol. 8, no. 6, 2021.

Abstract | Links | BibTeX

X. Chamling; A. Kallman; W. Fang; C. Berlinicke; J. Mertz; P. Devkota; I.M. Pantoja; M. Smith; Z. Ji; C. Chang; A. Kaushik; L. Chen; K. Whartenby; P. Calabresi; H.Q. Mao; H. Ji; T.H. Wang,; D. Zack

Single-Cell Transcriptomic Reveals Molecular Diversity and Developmental Heterogeneity of Human Stem Cell-derived Oligodendrocyte Lineage Cells Journal Article

In: Nature Communications, vol. 12, no. 652, 2021.

Abstract | Links | BibTeX

F.E. Chen; A. Kaushik; K. Hsieh; E. Chang; L. Chen; P. Zhang; T.H. Wang

Toward Decentralizing Antibiotic Susceptibility Testing via Ready-to-Use Microwell Array and Resazurin-Aided Colorimetric Readout Journal Article

In: Analytical Chemistry, vol. 93, no. 3, pp. 1260–1265, 2021.

Abstract | Links | BibTeX

B. Scherer; C. Surrette; H. Li,; P. Torab,; E. Kvam; C. Galligan; S. Go; G. Grossmann; T. Hammond; T. Johnson; R. St-Pierre; J. Nelson; R.A. Potyrailo; T. Khire; K. Hsieh; T.H. Wang; P. Kin Wong; C. Puleo

Digital electrical impedance analysis for single bacterium sensing and antimicrobial susceptibility testing Journal Article

In: Lab on a Chip, 2021.

Abstract | Links | BibTeX

186 entries « 1 of 5 »

Top

Patents

  1. H. Wang, F.M. Friedrich, J.M. Burke, and K. Liu “Concentration and Accumulation of Target Species in Response to a Gradient of Solute or Solvent” (JHU Ref. C13791; US provisional patent)
  2. H. Wang, K. Hsieh, H. Zec, L. Liu, A.M. Kaushik, Y. Yun, “Multiplexed, Continuous-flow, droplet-based platform for high-throughput genetic detection” (JHU Ref. C13529; US provisional patent)
  3. H. Wang, T.D. Rane, H.C Zec, “System and device for high throughput generation of combinatorial droplets and methods of use”  (PCT/US2015/012927; JHU Ref. C12862)
  4. H. Wang, D.J. Shin, “Self-contained cartridge and methods for integrated biochemical assay at the point-of-care” (JHU Ref. C12720)
  5. -H. Wang, S. Yang, M.A. Jacobs, P. Athamanolap, S.I. Fraley, V. Agarwal, V. Parekh. “Melt curve classifier for reliable large-scale genotyping of sequence variants” JHU Case Number C12600, provisional patent
  6. Stephanie I. Fraley, T.-H. Wang, and S. Yang. “A novel technology for broad-based, yet single molecule sensitive profiling in heterogeneous biological samples.” JHU Case Number C12403, provisional patent
  7. H. Wang, Y. Zhang, “Fabrication of Hierarchical Silica Nanomembrane using Heat-Shrinking Polymers and Applications of Silica Nanomembrane for the Solid Phase Extraction of Nucleic Acids”, JHU Case Number C12404, provisional patent
  8. H. Wang, Chi-Hang Chiou, Dong Jin Shin , “Electromagnetically Actuated Droplet Microfluidic Chip and System” (JHU Ref. C12262)
  9. H. Wang and Y. Zhang, “Surface Energy Traps (SETs) Enabled Complex Droplet Manipulation” (JHU Ref. C11971)
  10. H. Wang, K. Liu and Y. Song, “miRNA Analysis Method” (US Provisional US 61/598513, JHU Ref. C11885)
  11. H. Wang, T.D. Rane, H. C. Zec and W.Ch. Chu “Systems and methods for screening a library of samples” (US13/708,510. JHU Ref. C11803)
  12. Yang, T.H. Wang, S.K. Park and Y Zhang, “Method and apparatus for continuous microfluidic sample separation and concentration using AC electric field” (JHU Ref. C11562)
  13. H. Wang, Y. Zhang, S. Park and S. Yang, “Self-sustained Droplet Microfluidic Chip and System for Integrated Sample Preparation and Nucleic Acids Detection” (PCT/US2011/045363, JHU Ref. C11183)
  14. H. Wang, H.Q. Mao, W. Beh and D. Kraitchman, “Kilohertz Generation of Monodisperse Microbeads on a Polymeric Membrane Microfluidic Pseudocheck Valve Device” (PCT/US2011/054598, JHU Ref. C11248)
  15. Ahuja, V. Bailey, S.B. Baylin, J.G. Herman, T.H. Wang, J. Yi, “Early Detection of DNA Methylation Biomarker in Cancer Patient Sera” (PCT/US2011/037926, JHU Ref. C11091)
  16. H. Wang and K. Liu, “Hydrodynamic Particle Separation and Detection Systems and Methods”, (PCT/US2011/056941, JHU Ref. C11263)
  17. H. Wang and K.J. Liu, “Single Molecule Spectroscopy for Analysis of Cell-free Nucleic Acid Biomarkers” (PCT/US2010/033888, JHU Ref. C10750)
  18. H. Wang and Y. Zhang, ” Device and Method of Preparing and Performing Multiple Polymerase Chain Reactions” (US 12/716,031, JHU Ref. C10659)
  19. H. Wang, S. Baylin, J. Herman, H. Easwaran and H. Carraway, “Compositions and Methods for Polynucleotide Extraction and Methylation Detection” (PCT/US2009/000039, JHU Ref. C10249)
  20. M. Ho and T.H. Wang, “Biosensors and Methods for Their Use” (PCT/US2001/025444)
  21. H. Wang, K.J. Liu, and I.M. Shih, ” DNA Integrity Assay (DIA) for Cancer Diagnostics, Using Confocal Fluorescence Spectroscopy” (US8,835,110, JHU Ref. C10534)
  22. H. Wang and K. Liu, “Cylindrical Illumination Confocal Spectroscopy System” (US8,248,609, JHU Ref. C10398)
  23. H. Wang, K.J. Liu, C.M. Puleo and T. Rane, ” Microfluidic System for High-Throughput, Droplet-based, Single Molecule Analysis with Low Reagent Consumption” (US9,284,601, issued on 03/15/2016, JHU Ref. C10662)
  24. F. Miller, J. Huang, T.H. Wang, C.M. Ho and M. Liu, “Electrochemical Detection of Mismatch in Nucleic Acids” (US 7,291,457)
  25. H. Wang, “Method for determining standard cycle time of a stage dynamically” (US5,825,650)
  26. A. Hulbert, J. Herman, M. Brock, T.H. Wang, and A. Stark “Early detection of Individuals at High Risk for Lung Cancer of all Stages Using Gene promoter Methylation in Plasma and Sputum” JHU C13599

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Conference Publications

  1. J. Shin, P. Athamanolap, L.Chen, J. Hardick, CA Gaydos and T.H. Wang, “A Smartphone-Based Mobile NAAT Diagnostic Suite for Chlamydia Detection”, Proc. 26th Anniversary World Congress on Biosensors (Biosensors 2016), (accepted)
  2. M. Friedrich, J.M. Burke, K.J. Liu, T.H. Wang, “In-line Preconcentration, Size Separation, and Single-Molecule Detection Without Applied Electric Fields, Proc. 29th IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2016), p. 181-184, 2016
  3. J. Shin, L. Chen and T.H. Wang, “Single-bacteria Confocal Spectroscopy: An Ultrasensitive method for Real-time Monitoring of Bacterial Growth”, Proc. 19th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2015), p. 340-342, 2015
  4. Kaushik, K. Hsieh, L. Chen, D.J. Shin and T.H. Wang, “Rapid Assessment of Bacterial Vitality and Antibiotic Susceptibility via High-Throughput Picoliter-Droplet Single-Cell Assay”, Proc. 19th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2015), p. 531-533, 2015
  5. Liu, K. Hsieh, A. Kaushik, H.C. Zec and T.H. Wang, “Multiplexed, Continuous-Flow, Droplet-Based PCR Genotyping Platform for High-Throughput Agriculture Marker Assisted Selection”, Proc. 19th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2015), p. 1368-1370, 2015
  6. Zec, C. O’Keefe, P. Ma, T.H. Wang, “Ultra-Thin, Evaporation-Resistant PDMS Devices for Absolute Quantification of DNA Using Digital PCR”, The 18th International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers 2015), p.536-539, 2015
  7. Guan, L. Chen, T. Rane, A. Kaushik, T.H. Wang, “Digital Droplet ELOHA For Nucleic Molecule Counting And Analysis”, The 18th International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers 2015), p.536-5460-463, 2015
  8. C. Zec, C.J. Glover, W. Hsieh, L. Liu, C. O’Keefe and T.H. Wang, “Methods for Controlling Water Evaporation in PDMS-Based Microfluidic Devices”, Proc. 18th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2014), p. 1743-1745, 2014
  9. J. Shin, P. Athamanonolap, L. Chen and T.H. Wang, “Integrated droplet microfluidic platform for nucleic acids amplification test of Chlamydia trachomatis infection”, Proc. 24th Anniversary World Congress on Biosensors (Biosensors 2014)
  10. C. Zec, T.D. Rane, P. Ma and T.H. Wang, “Parallelization of Fission and Fusion- Operations for High Throughput Generation of Combinatorial Droplets”, Proc. 27th IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2014), p. 334-337, 2014
  11. J. Shin, L. Chen and T.H. Wang, “A Simple Integrated Diagnostic Platform for DNA Testing of Chlamydia Trachomatis Infection, “ Proc. 17th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2013), p. 1350-1352, 2013
  12. M. Friedrich, K.J. Liu and T.H. Wang, “ Single Molecule Hydrodynamic Separation for Ultrasensitive and Quantitative DNA Size Separations,” Proc. 17th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2013), p. 35-37, 2013
  13. D. Rane, H.C. Zec and T.H. Wang, “A Multiplexed Microfluidic Droplet Platform for Matrix Metalloproteinase Screening”, Proc. 17th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2013), p. 1595-1597, 2013
  14. K. Wu, S. F. Friedrich, K.J. Liu and T.H. Wang, “ Chip-Based DNA Separation in Free Solution by Inertial Hydrodynamic Forces”, Proc. 17th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2013), p. 578-580, 2013
  15. J. Shin, A. Stark and T.H. Wang, “ Droplet Bisulfite Conversion Platform for Epigenetic Cancer Biomarker Detection”, The 17th International Conference on Solid-State Sensors, Actuators and Microsystems(Transducers 2013), p.2181-2184, 2013
  16. Zhang, Y. Zhang, B. Keeley, A. Stark and T.H. Wang, “Spontaneous Shrinking Silica Nanomembrane for Solid Phase”, Proc. 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (IEEE NEMS 2013), p. 444-445, 2013
  17. Athamanolap, B. Keeley, D.J. Shin and T.H. Wang, “Quantitative Analysis of DNA Methylation Based on Melting Curve Analysis”, Proc. 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (IEEE NEMS 2013), p.1116-1118, 2013
  18. Zhang, Y Zhang, T.H. Wang, “Hierarchical Silica Nanomembrane Driven by Thermal Shrinkage and its Application for Solid Phase DNA Extraction”, Proc. The 13th IEEE International Conference on Nanotechnology (IEEE NANO 2013), 2013
  19. C. Zec, T.D. Rane, W.C. Chu, V. Wang and T.H. Wang, “ A Microfluidic Droplet Platform for Multiplexed Single Nucleotide Polymorphism Analysis of an Array Plant Genomic DNA Samples”, Proc. 26th IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2013), p.263-266, 2013
  20. H. Chiou, D.J. Shin, S. Hosmane, Y Zhang and T.H. Wang, “Electromagnet-Actuated Droplet Platform for Sample-to-Answer Genetic Detection”, Proc. 26th IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2013), p.98-101, 2013
  21. Zhang and T.H. Wang, “ All-in-One Droplet Platform for Multiplexed Genetic Detection in Blood” Proc. 26th IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2013), p. 1061-1064, 2013
  22. Zhang and T.H. Wang, “Flip-Drop: Droplet Array Created by Surface Energy Trap for Combinatorial Screening,” Proc. 26th IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2013), p.1149-1152, 2013
  23. W. Beh, C. Weiss, H.Q. Mao, D. L. Kraitchman, and T.H. Wang, “High-Throughput Microfluidic Preparation of Imaging-Visible Embolic Beads”, Proc. EMBS Micro and Nanotechnology in Medicine Conference, p.59, 2012
  24. Zhang, Y. Zhang, B. Keeley, A. Stark and T.H. Wang, “Fabricating and Applying Hierarchical Silica Nanomembrane with for Solid Phase DNA Extraction” The 6th IEEE International Conference on Nano/Molecular Medicine and Engineering (IEEE NANOMED 2012), 2012
  25. Keeley, Yi Zhang, Ye Zhang, A. Stark, T.H. Wang, “Quantum Dot FRET Linker Probes for Highly Sensitive DNA Methylation Detection”, Proc. IEEE 12th International Conference on Nanotechnology (IEEE NANO 2012), 7848, p.1-4, 2012
  26. Zhang and T.H. Wang, “Quantum Dot Electrophoretic Mobility Shift Assay and Its Application to the Measurement of Exonuclease Activity” Proc. IEEE 12th International Conference on Nanotechnology (IEEE NANO 2012), 7685, p.1-4, 2012
  27. Song, L. Zhang, M. Chen and T.H. Wang, “Single Quantum Dot Fluorescence Enhancement by Tunable Nanoporous Gold”, Proc. IEEE 12th International Conference on Nanotechnology (IEEE NANO 2012), 7877, p.1-4, 2012
  28. Zhang and T.H. Wang, “Droplet Immobilization, Splitting, Metering and Aliquoting with Surface Energy Traps Created Using SU8 Shadow Mask” Proc. 16th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2012), p. 73-75, 2012
  29. Zhang and T.H. Wang, “Surface Energy Trap Assisted Rapid Serial Dilution on Droplet Platform for Bacteria Antibiotics Susceptibility Test” Proc. 16th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2012), p. 467-469, 2012
  30. J. Liu, T.D. Rane, Y. Zhang, C. Beh, D.J. Shin, T.H. Wang, “An Integrated Platform for Single Molecule Free Solution Hydrodynamic Separation Using Yoctomoles of DNA and Picoliter Samples”, ASME 10th International Conference on Nanochannels, Microchannels and Minichannels (ICNMM 2012), ICNMM2012-73154 (p.1-6), 2012
  31. Zec, T.D. Rane, W.C. Chu and T.H. Wang, “Multiplexed Screening of a Large Library of Biological Samples through on-Demand Droplet Generation and Fusion”, ASME 2012 10th International Conference on Nanochannels, Microchannels and Minichannels (ICNMM 2012), ICNMM2012-73159 (p.1-6), 2012
  32. Song, Y. Zhang, and T.H. Wang, “Single Quantum Dot-Based Multiplexed Point Mutation Detection by Gap Ligase Chain Reaction”, Proc. 15th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2011), p.1779-1781, 2011
  33. Zhang, D.J. Shin and T.H. Wang, “Detecting Genetic Variations in A Droplet”, Proc. 15th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2011), p.1179-1181, 2011
  34. J. Liu and T.H. Wang, “ PCR-free, microfluidic single molecule analysis of circulating nucleic acids in lung cancer patient serum”, Proc. 33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC ’11), p.8392-8395, 2011
  35. Zhang and T.H. Wang, “ An Active Gyroscopic Magnetic Micromixer for Rapid Fluid Mixing in Droplet Based Microfluidic Systems”, The 16th International Conference on Solid-State Sensors, Actuators and Microsystems(Transducers 2011), p.1769-1772, 2011
  36. Zhang, S.K. Park, S. Yang and T.H. Wang, “Fully Integrated Droplet Based Point-of-Care Platform for Molecular Detection from Crude Biosamples”, The 16th International Conference on Solid-State Sensors, Actuators and Microsystems(Transducers 2011), p.1927-1930, 2011
  37. H. Wang, V. Bailey and K. Liu, “Quantum Dots and Microfluidic Single Molecule Detection for Screening Genetic and Epigenetic Cancer Markers in Clinical Samples”, Proc. 2011 SPIE Defense, Security and Sensing Conference, Volume 8031, P. 80311W
  38. D. Rane, H. Zec, C.M. Puleo, A.P. Lee and T.H. Wang, “High-Throughput Single-Cell Pathogen Detection on a Droplet Microfluidic Platform”, Proc. 24th IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2011), p.881-884, 2011
  39. Stark, Y. Zhang, V. Bailey, B. Keeley. T.H. Wang, “Increasing Throughput and Sensitivity of DNA Methylation Analysis through Functional Nanoparticles” The 6th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (IEEE NEMS 2011), 1091-1094, 2011
  40. Y Zhang and T.H. Wang, “A Quantum Dot Based Electrophoretic Mobility Shift Assay for High Resolution Copy Number Variation Study”, The 6th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (IEEE NEMS 2011), p.841-844, 2011
  41. Zhang, I.M. Shih, T.L. Wang and T.H. Wang, ” A Quantum Dot Based Nanoassay for Quantifying Gene Copy Number with Ultrahigh Resolution”, Proc. 14th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2010), p.1154-1156, 2010
  42. W. Beh, W. Zhou and T.H. Wang, “Oxygen Plasma-Free Microfluidic Device Sealing”, Proc. 14th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2010) p.1217-1219, 2010
  43. W. Beh, D. Kraitchman, H.Q. Mao, T.H. Wang, “High-throughput Monodisperse Alginate Gel Bead Formation using Microfluidic Pseudo-Check Valve”, Proc. 14th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2010) p.425-427, 2010
  44. Zhang and T.H. Wang, “An Automated All-in-one Microfluidic Device for Parallel Solid Phase DNA Extraction and Droplet-in-Oil PCR Analysis”, Proc. 23rd IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2010), P.971-974, 2010
  45. Zhang and T.H. Wang, “Geomorphology-Assisted Manopulation of Magnet-Actuated Droplet for Solid Phase DNA Extraction and Droplet-in-Oil PCR”, Proc. 23rd IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2010), p.1047-1050, 2010
  46. D. Rane, C.M. Puleo, H. Zec, Y. Zhang, A.P. Lee and T.H. Wamh, “Analyte Detection in Droplets: One Molecule at a Time”, Proc. 13th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2009), p.15-17, 2009
  47. M. Puleo, H.C. Zec, Y. Sung and T.H. Wang, “Micro-evaporator as Interconnects to Low-Volume Microfluidic Components”, Proc. 13th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2009), p.932-935, 2009
  48. J. Bailey, C.M. Puleo, Y.P. Ho, H.C. Yeh, T.H. Wang, “Quantum Dots in Molecular Detection of Diseases”, 31st Annual International Conference on the IEEE Engineering in Medicine and Biology Society ( IEEE EMBC 2009), p. 4089-4092, 2009
  49. Zhang, V. Bailey, C.M. Puleo, H. Easwaran, E. Griffiths, J.G. Herman, S.B. Baylin, T.H. Wang, “High Throughput DNA Methylation Analysis on a Droplet-in-Oil Polymerase Chain Reaction Array”, The 15th International Conference on Solid-State Sensors, Actuators and Microsystems(Transducers 2009), p.806-808, 2009
  50. Zhang, V. Bailey, C.M. Puleo, C. Chen and T.H. Wang, “Multiple gene Analysis within a Simple Droplet-in-Oil Microfluidic PCR Platform”, Proc. 12th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2008), p. 751-753 , 2008
  51. P. Ho, H.H. Chen, K. Leong and T.H. Wang “Quantitative Kinetic Analysis of DNA Nanocomplex Self-Assembly with Quantum Dots FRET in a Microfluidic Device”, Proc. 21st IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2008), p. 30-33, 2008
  52. M. Puleo, H.C. Yeh and T.H. Wang “Single Molecule Detection in Truly, Nanoliter-Sized Volumes: Coupling Evaporation-Based, Microfluidic Concentration with Confocal Fluorescence Spectroscopy” Proc. 21st IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2008), p.200-203, 2008
  53. Liu and T.H. Wang, “Quantitative Confocal Spectroscopy – Rectifying the Limitations of Single Molecule Detection”, Proc. 3rd Annual IEEE International Conferences on Nano/Micro Engineered and Molecular Systems (IEEE-NEMS 2008), p. 1189-1192, 2008
  54. C. Yeh. Y.P. Ho, C.M. Puleo and T.H. Wang,” Towards single-molecule diagnostics using microfluidic manipulation and quantum dot nanosensors, Proc. 5th International Conference on Nanochannels, Microchannels and Minichannels, (ICNMM 2007), p. 1133-1140, 2007
  55. P. Ho and T.H. Wang, “Multiplexed Detection of Anthrax Sequences with Quantum Dot Nanoprobes”, Proc. IEEE/NLM Life Science Systems and Application Workshop, p. 62-63, 2006
  56. Murray , K. Rebello, J. Crookston, J. Miragliotta and T.H. Wang, “High-degree Concentration of Bio-agents Using Electrokinetic Manipulations”, Proc. IEEE/NLM Life Science Systems and Application Workshop, p. 94-95, 2006
  57. P. Ho, M.C. Kung and T.H. Wang, ” Separation-free Detection of Low-abundant Biomolecules with Two-Color Colocalization of Quantum Dot Probes,” Proc. 9th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2005), p. 1330-1332, 2005
  58. C. Yeh, Y.P. Ho and T.H. Wang, “Quantum Dot-Mediated Separation-Free Assay for Point Mutation Detection ” Proc. NSTI- Bio-Nanotechnology Conference, 198-201, 2005
  59. C. Yeh, E. Simone, C.Y. Zhang and T.H. Wang, “Single Bio-Molecule Detection with Quantum-dots in Flow-rate Controlled Microchannel”, Proc. 17h IEEE Annual Workshop of Micro Electro Mechanical Systems (IEEE MEMS 2004), p 371-374, 2004
  60. Y. Chao, C.Y. Zhang and T.H. Wang,” Measurement of in-situ Flow Velocity Using Single-Molecule Detection for the Application of Biomolecule Quantification”, Proc. Hilton Head 2004 Solid-State Sensor, Actuator, and Microsystems Workshop (Hilton Head 2004), p. 176-179, 2004
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  63. K. Wong, C.Y. Chen, T.H. Wang and C.M. Ho, “An AC Electroosmotic Processor for Biomolecules,” Proc. 12th International Conference on Solid-state Sensors, Actuators, and Microsystems (Transducers 2003), vol. 1, p. 20-23, 2003
  64. K. Wong, T.H. Wang and C.M. Ho, “Optical Fiber Tip Fabricated by Surface Tension Controlled Etching”, Proc. Hilton Head 2002: Solid-State Sensor, Actuator, and Microsystems Workshop (Hilton Head 2002), p. 94-97, 2002
  65. H. Wang, P.K. Wong and C.M. Ho, “Electrical Molecular Focusing for Laser Induced Fluorescence Based Single DNA Detection”, Proc. 15th IEEE International Conference on Micro Electro Mechanical Systems(IEEE MEMS 2002), p. 15-18, 2002
  66. H. Wang, S. Masset and C.M. Ho, “A Zepto Mole DNA Micro Sensor,” Proc. 14th IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS),  p. 431-434, 2001
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