To get started, you will need to take a few training classes to be certified to work in the BL2 lab.

1. The online bloodborne pathogen training that can be accessed by logging into your JHED account and clicking on myLearning in the Education tab. Please copy your certification paper on Allegheny/shared/LabDocuments/BioLabRegistration.
2. There is also a lab training class that is highly recommended if you have never worked in a lab, called Laboratory Safety. It is an hour class at the Medical School. Class info can be found http://www.hopkinsmedicine.org/hse/training/
3. Contact Vicky Nguyen for safety training for the BL2 Lab in Latrobe 216 A. Read the Guidelines for Working in BL2 Latrobe 216A Lab.
4. Read the Safety Incident Report page. Once you have successfully completed these 4 steps, you are good to get started! NOT BEFORE!!!!!

EMERGENCY PROCEDURES: Report your emergency to the following appropriate authorities. Write a report of your lab accident on the Safety Incident Report wiki. Download, fill out and submit the following safety incident report.

incrpt.pdf

• Blood exposure: call from the lab phone: 5-7849 (or 5-STIX). If you call from your phone, dial: 410 955 7849
• Chemical exposure: 5-64333
• Chemical spill: 5-4444
• Chemical disposal: 5-5918 check out the website: www.hopkinsmedecine.org/hse/emergency
• RECYCLING OF BIOHAZARD SOLID WASTES The biohazard waste boxes have to be disposed at the south gate of Latrobe Hall (in front of Barton Hall).
• Send an email to Richarch Abraham: rabraha5@jhu.edu Do not do that on Friday.

Your safety training program has three parts:

1. A general introduction to basic laboratory safety - Online training. If you have not done so already, you need to take the basic lab safety online training. Go to myjhu, you will need a JHED ID. In Education, go to my learning. Look for and select Laboratory Safety Introductory Course.
2. FOR EVERYBODY: a training in the use of cryogenics and at least, how to use the oxygen monitor. If nobody in the lab is able to do so, the lab safety office for the Whiting School of Engineering can provide a LN2 safety training.
3. A safety orientation for each specific equipment you want to use, performed by somebody from the lab.
   • DMA dynamic mechanical analysis: Jingkai Guo
   • MTS tensile machine and environmental chamber: Jingkai Guo
   • Liquid Nitrogen use: Jingkai Guo
   • Smaller equipments (balance, oxygen monitor, oven, incubator, hood): Manual available on the Wiki (section Manuals/Smaller equipment). Read it!

Step 3 entails a tracking sheet to be signed by you and the person training you for each specific equipment/procedures you are planning to use/perform. This sheet should be left in the lab.

Print the following document to start training

training_sheet.pdf

Do not attempt to use an equipment without having been trained on the dangers and associated safety procedures corresponding to that equipment.

Each equipment you want to use needs to be booked through the google calendar of the lab (called DMA). During your in-person training, ask the person training you to share the calendar with you (need a gmail address). Please book the machine only for the hours you will be present and using it. If the machine is still available 30 minutes after the beginning of your slot, it is up for grabs.

Safety Incident Report- Read as part of the training

incrpt.pdf