{"id":11,"date":"2016-05-02T04:22:59","date_gmt":"2016-05-02T04:22:59","guid":{"rendered":"http:\/\/localhost\/biomems\/?page_id=11"},"modified":"2020-05-01T07:47:13","modified_gmt":"2020-05-01T11:47:13","slug":"publications","status":"publish","type":"page","link":"https:\/\/me.jhu.edu\/thwang\/publications.html","title":{"rendered":"Publications"},"content":{"rendered":"<p style=\"text-align: right;\"><a href=\"#top\">Journal Publications<\/a> <a href=\"#Patents\">| Patents<\/a> <a href=\"#Conference\">| Conference Publications<\/a><\/p>\n<div class=\"lists\">\n<h3>Journal Publications<\/h3>\n<p><div class=\"teachpress_pub_list\"><form name=\"tppublistform\" method=\"get\"><a name=\"tppubs\" id=\"tppubs\"><\/a><div class=\"tp_search_input\"><input name=\"tsr\" id=\"tp_search_input_field\" type=\"search\" placeholder=\"Enter search word\" value=\"\" tabindex=\"1\"\/><div class=\"teachpress_search_button\"><input name=\"tps_button\" class=\"tp_search_button\" type=\"submit\" tabindex=\"10\" value=\"Search\"\/><\/div><\/div><\/form><div class=\"tablenav\"><div class=\"tablenav-pages\"><span class=\"displaying-num\">212 entries<\/span> <a class=\"page-numbers button disabled\">&laquo;<\/a> <a class=\"page-numbers button disabled\">&lsaquo;<\/a> 1 of 11 <a href=\"https:\/\/me.jhu.edu\/thwang\/publications.html?limit=2&amp;tgid=&amp;yr=&amp;type=&amp;usr=&amp;auth=&amp;tsr=\" title=\"next page\" class=\"page-numbers button\">&rsaquo;<\/a> <a href=\"https:\/\/me.jhu.edu\/thwang\/publications.html?limit=11&amp;tgid=&amp;yr=&amp;type=&amp;usr=&amp;auth=&amp;tsr=\" title=\"last page\" class=\"page-numbers button\">&raquo;<\/a> <\/div><\/div><div class=\"teachpress_publication_list\"><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_number\">1.<\/div><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">T. Wu D.J.M. Park, L. Chen;  Wang, T. H.<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7280','tp_links')\" style=\"cursor:pointer;\">Simple dual filter workflow for facilitating blood culture-free and sensitive detection of pathogenic bacteria from blood<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Scientific Reports, <\/span><span class=\"tp_pub_additional_volume\">vol. 15, <\/span><span class=\"tp_pub_additional_issue\">iss. 1, <\/span><span class=\"tp_pub_additional_number\">no. 24766, <\/span><span class=\"tp_pub_additional_year\">2025<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7280\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7280','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7280\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7280','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7280\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Park2025,<br \/>\r\ntitle = {Simple dual filter workflow for facilitating blood culture-free and sensitive detection of pathogenic bacteria from blood},<br \/>\r\nauthor = {D.J.M. Park, T. Wu, L. Chen, P.W. Lee, K. Hsieh and T.H. Wang},<br \/>\r\ndoi = {https:\/\/doi.org\/10.1038\/s41598-025-08987-z},<br \/>\r\nyear  = {2025},<br \/>\r\ndate = {2025-07-09},<br \/>\r\nurldate = {2025-07-09},<br \/>\r\njournal = {Scientific Reports},<br \/>\r\nvolume = {15},<br \/>\r\nnumber = {24766},<br \/>\r\nissue = {1},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7280','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7280\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/https:\/\/doi.org\/10.1038\/s41598-025-08987-z\" title=\"Follow DOI:https:\/\/doi.org\/10.1038\/s41598-025-08987-z\" target=\"_blank\">doi:https:\/\/doi.org\/10.1038\/s41598-025-08987-z<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7280','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_number\">2.<\/div><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">J. Hu J.S. Park, L. Chen;  Wang, T. H.<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7281','tp_links')\" style=\"cursor:pointer;\">FlexPCR: A Streamlined Multiplexed Digital mRNA Quantification Platform with Universal Primers and Limited Fluorescence Channels<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Biosensors and Bioelectronics, <\/span><span class=\"tp_pub_additional_volume\">vol. 277, <\/span><span class=\"tp_pub_additional_pages\">pp. 117277, <\/span><span class=\"tp_pub_additional_year\">2025<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7281\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7281','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7281\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7281','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7281\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Park2025b,<br \/>\r\ntitle = {FlexPCR: A Streamlined Multiplexed Digital mRNA Quantification Platform with Universal Primers and Limited Fluorescence Channels},<br \/>\r\nauthor = {J.S. Park, J. Hu, L. Chen, and T.H. Wang},<br \/>\r\nurl = {https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0956566325001514},<br \/>\r\ndoi = {https:\/\/doi.org\/10.1016\/j.bios.2025.117277},<br \/>\r\nyear  = {2025},<br \/>\r\ndate = {2025-06-01},<br \/>\r\njournal = {Biosensors and Bioelectronics},<br \/>\r\nvolume = {277},<br \/>\r\npages = {117277},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7281','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7281\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0956566325001514\" title=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0956566325001514\" target=\"_blank\">https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0956566325001514<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/https:\/\/doi.org\/10.1016\/j.bios.2025.117277\" title=\"Follow DOI:https:\/\/doi.org\/10.1016\/j.bios.2025.117277\" target=\"_blank\">doi:https:\/\/doi.org\/10.1016\/j.bios.2025.117277<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7281','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_number\">3.<\/div><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">M. Totten P.W. Lee, A. Traylor;  Hsieh, K.<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7287','tp_links')\" style=\"cursor:pointer;\">Cross-Kingdom Pathogen Detection via Duplex Universal PCR and High-Resolution Melt<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Biosensors and Bioelectronics, <\/span><span class=\"tp_pub_additional_volume\">vol. 270, <\/span><span class=\"tp_pub_additional_number\">no. 116922, <\/span><span class=\"tp_pub_additional_year\">2025<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7287\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7287','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7287\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7287','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7287\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Lee2025,<br \/>\r\ntitle = {Cross-Kingdom Pathogen Detection via Duplex Universal PCR and High-Resolution Melt},<br \/>\r\nauthor = {P.W. Lee, M. Totten, A. Traylor, S.X. Zhang, T.H. Wang and K. Hsieh},<br \/>\r\nurl = {https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0956566324009291},<br \/>\r\ndoi = {https:\/\/doi.org\/10.1016\/j.bios.2024.116922},<br \/>\r\nyear  = {2025},<br \/>\r\ndate = {2025-02-15},<br \/>\r\nurldate = {2025-02-15},<br \/>\r\njournal = {Biosensors and Bioelectronics},<br \/>\r\nvolume = {270},<br \/>\r\nnumber = {116922},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7287','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7287\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0956566324009291\" title=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0956566324009291\" target=\"_blank\">https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0956566324009291<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/https:\/\/doi.org\/10.1016\/j.bios.2024.116922\" title=\"Follow DOI:https:\/\/doi.org\/10.1016\/j.bios.2024.116922\" target=\"_blank\">doi:https:\/\/doi.org\/10.1016\/j.bios.2024.116922<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7287','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_bachelorthesis\"><div class=\"tp_pub_number\">4.<\/div><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">C.M. O&#039;Keefe Y. Zhao, K. Hsieh<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7311','tp_links')\" style=\"cursor:pointer;\">Multiplex Digital Methylation\u2010Specific PCR for Noninvasive Screening of Lung Cancer<\/a> <span class=\"tp_pub_type tp_  bachelorthesis\">Bachelor Thesis<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_year\">2024<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7311\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7311','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7311\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7311','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7311\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@bachelorthesis{Zhao2024b,<br \/>\r\ntitle = {Multiplex Digital Methylation\u2010Specific PCR for Noninvasive Screening of Lung Cancer},<br \/>\r\nauthor = {Y. Zhao, C.M. O&#039;Keefe, K. Hsieh, L. Cope, S.C. Joyce, T.R. Pisanic, J.G. Herman, T.H. Wang},<br \/>\r\nurl = {https:\/\/www.science.org\/doi\/10.1126\/sciadv.adp1704},<br \/>\r\ndoi = {DOI: 10.1126\/sciadv.adp1704},<br \/>\r\nyear  = {2024},<br \/>\r\ndate = {2024-11-22},<br \/>\r\njournal = {Advanced Science},<br \/>\r\nvolume = {10},<br \/>\r\nissue = {47},<br \/>\r\npages = {2206518},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {bachelorthesis}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7311','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7311\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/www.science.org\/doi\/10.1126\/sciadv.adp1704\" title=\"https:\/\/www.science.org\/doi\/10.1126\/sciadv.adp1704\" target=\"_blank\">https:\/\/www.science.org\/doi\/10.1126\/sciadv.adp1704<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/DOI: 10.1126\/sciadv.adp1704\" title=\"Follow DOI:DOI: 10.1126\/sciadv.adp1704\" target=\"_blank\">doi:DOI: 10.1126\/sciadv.adp1704<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7311','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_number\">5.<\/div><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">C.M. O\u2019Keefe Y. Zhao, J. Hu<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7283','tp_links')\" style=\"cursor:pointer;\">Multiplex digital profiling of DNA methylation heterogeneity for sensitive and cost-effective cancer detection in low-volume liquid biopsies<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Science Advances, <\/span><span class=\"tp_pub_additional_volume\">vol. 10, <\/span><span class=\"tp_pub_additional_issue\">iss. 47, <\/span><span class=\"tp_pub_additional_year\">2024<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7283\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7283','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7283\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7283','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7283\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Zhao2024,<br \/>\r\ntitle = {Multiplex digital profiling of DNA methylation heterogeneity for sensitive and cost-effective cancer detection in low-volume liquid biopsies},<br \/>\r\nauthor = {Y. Zhao, C.M. O\u2019Keefe, J. Hu, C.M. Allan, W. Cui, H. Lei, A. Chiu, K. Hsieh, S.C. Joyce, J.G. Herman, T.R. Pisanic, T.H. Wang},<br \/>\r\nurl = {https:\/\/www.science.org\/doi\/10.1126\/sciadv.adp1704},<br \/>\r\ndoi = {DOI: 10.1126\/sciadv.adp1704},<br \/>\r\nyear  = {2024},<br \/>\r\ndate = {2024-11-22},<br \/>\r\njournal = {Science Advances},<br \/>\r\nvolume = {10},<br \/>\r\nissue = {47},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7283','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7283\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/www.science.org\/doi\/10.1126\/sciadv.adp1704\" title=\"https:\/\/www.science.org\/doi\/10.1126\/sciadv.adp1704\" target=\"_blank\">https:\/\/www.science.org\/doi\/10.1126\/sciadv.adp1704<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/DOI: 10.1126\/sciadv.adp1704\" title=\"Follow DOI:DOI: 10.1126\/sciadv.adp1704\" target=\"_blank\">doi:DOI: 10.1126\/sciadv.adp1704<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7283','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_number\">6.<\/div><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">P. Zhang J. Hu, F. Shao<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7285','tp_links')\" style=\"cursor:pointer;\">A streamlined proximity extension assay using POEGMA polymer-coated magnetic beads for enhanced protein detection<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Frontiers in Bioengineering and Biotechnology, <\/span><span class=\"tp_pub_additional_volume\">vol. 12, <\/span><span class=\"tp_pub_additional_number\">no. 1462203, <\/span><span class=\"tp_pub_additional_year\">2024<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7285\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7285','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7285\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7285','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7285\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Hu2024,<br \/>\r\ntitle = {A streamlined proximity extension assay using POEGMA polymer-coated magnetic beads for enhanced protein detection},<br \/>\r\nauthor = {J. Hu, P. Zhang, F. Shao, T.H. Wang},<br \/>\r\nurl = {https:\/\/www.frontiersin.org\/journals\/bioengineering-and-biotechnology\/articles\/10.3389\/fbioe.2024.1462203\/full},<br \/>\r\ndoi = {https:\/\/doi.org\/10.3389\/fbioe.2024.1462203},<br \/>\r\nyear  = {2024},<br \/>\r\ndate = {2024-11-20},<br \/>\r\njournal = {Frontiers in Bioengineering and Biotechnology},<br \/>\r\nvolume = {12},<br \/>\r\nnumber = {1462203},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7285','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7285\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/www.frontiersin.org\/journals\/bioengineering-and-biotechnology\/articles\/10.3389\/fbioe.2024.1462203\/full\" title=\"https:\/\/www.frontiersin.org\/journals\/bioengineering-and-biotechnology\/articles\/1[...]\" target=\"_blank\">https:\/\/www.frontiersin.org\/journals\/bioengineering-and-biotechnology\/articles\/1[...]<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/https:\/\/doi.org\/10.3389\/fbioe.2024.1462203\" title=\"Follow DOI:https:\/\/doi.org\/10.3389\/fbioe.2024.1462203\" target=\"_blank\">doi:https:\/\/doi.org\/10.3389\/fbioe.2024.1462203<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7285','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_number\">7.<\/div><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">L. Chen J. Hu, P. Zhang<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7289','tp_links')\" style=\"cursor:pointer;\">Exploiting \u03b2\u2010Lactams\u2010Induced Lysis and DNA Fragmentation for Rapid Molecular Antimicrobial Susceptibility Testing of Neisseria Gonorrhoeae via Dual\u2010Digital PCR<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Advanced Science, <\/span><span class=\"tp_pub_additional_volume\">vol. 11, <\/span><span class=\"tp_pub_additional_issue\">iss. 46, <\/span><span class=\"tp_pub_additional_number\">no. 2405272, <\/span><span class=\"tp_pub_additional_year\">2024<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7289\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7289','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7289\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7289','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7289\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Hu2024b,<br \/>\r\ntitle = {Exploiting \u03b2\u2010Lactams\u2010Induced Lysis and DNA Fragmentation for Rapid Molecular Antimicrobial Susceptibility Testing of Neisseria Gonorrhoeae via Dual\u2010Digital PCR},<br \/>\r\nauthor = {J. Hu, L. Chen, P. Zhang, F.E. Chen, H. Li, K. Hsieh, S. Li, J.H. Melendez, T.H. Wang},<br \/>\r\nurl = {https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/advs.202405272?msockid=3d93250867086f4720e3334666846ee6},<br \/>\r\ndoi = {https:\/\/doi.org\/10.1002\/advs.202405272},<br \/>\r\nyear  = {2024},<br \/>\r\ndate = {2024-10-18},<br \/>\r\njournal = {Advanced Science},<br \/>\r\nvolume = {11},<br \/>\r\nnumber = {2405272},<br \/>\r\nissue = {46},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7289','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7289\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/advs.202405272?msockid=3d93250867086f4720e3334666846ee6\" title=\"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/advs.202405272?msockid=3d93[...]\" target=\"_blank\">https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/advs.202405272?msockid=3d93[...]<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/https:\/\/doi.org\/10.1002\/advs.202405272\" title=\"Follow DOI:https:\/\/doi.org\/10.1002\/advs.202405272\" target=\"_blank\">doi:https:\/\/doi.org\/10.1002\/advs.202405272<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7289','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_number\">8.<\/div><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">S.Y. Tzeng Y. Hu, L. Cheng<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7291','tp_links')\" style=\"cursor:pointer;\">Supramolecular assembly of polycation\/mRNA nanoparticles and in vivo monocyte programming<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Proceedings of the National Academy of Sciences, <\/span><span class=\"tp_pub_additional_volume\">vol. 27, <\/span><span class=\"tp_pub_additional_issue\">iss. 121, <\/span><span class=\"tp_pub_additional_number\">no. 35, <\/span><span class=\"tp_pub_additional_pages\">pp. e2400194121, <\/span><span class=\"tp_pub_additional_year\">2024<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7291\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7291','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7291\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7291','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7291\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Hu2024c,<br \/>\r\ntitle = {Supramolecular assembly of polycation\/mRNA nanoparticles and in vivo monocyte programming},<br \/>\r\nauthor = {Y. Hu, S.Y. Tzeng, L. Cheng, J. Lin, A. Villabona-Rueda, S. Yu, S. Li, Z. Schneiderman, Y. Zhu, J. Ma, D.R. Wilson, S.R. Shannon, T. Warren, Y. Rui, C. Qiu, E.W. Kavanagh, K.M. Luly, Y. Zhang, N. Korinetz, F.R. D\u2019Alessio, T.H. Wang, E. Kokkoli, S.K. Reddy, E. Luijten, J.J. Green, H.Q. Mao},<br \/>\r\nurl = {https:\/\/pubmed.ncbi.nlm.nih.gov\/39172792\/},<br \/>\r\ndoi = {DOI: 10.1073\/pnas.2400194121},<br \/>\r\nyear  = {2024},<br \/>\r\ndate = {2024-08-22},<br \/>\r\njournal = {Proceedings of the National Academy of Sciences},<br \/>\r\nvolume = {27},<br \/>\r\nnumber = {35},<br \/>\r\nissue = {121},<br \/>\r\npages = {e2400194121},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7291','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7291\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/39172792\/\" title=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/39172792\/\" target=\"_blank\">https:\/\/pubmed.ncbi.nlm.nih.gov\/39172792\/<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/DOI: 10.1073\/pnas.2400194121\" title=\"Follow DOI:DOI: 10.1073\/pnas.2400194121\" target=\"_blank\">doi:DOI: 10.1073\/pnas.2400194121<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7291','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_number\">9.<\/div><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">Y. Zhao C.M. O&#039;Keefe, L. M. Cope<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7293','tp_links')\" style=\"cursor:pointer;\">Single-molecule epiallelic profiling of DNA derived from routinely collected Pap specimens for noninvasive detection of ovarian cancer<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Clinical and Translational Medicine, <\/span><span class=\"tp_pub_additional_volume\">vol. 14, <\/span><span class=\"tp_pub_additional_issue\">iss. 8, <\/span><span class=\"tp_pub_additional_pages\">pp. e1778, <\/span><span class=\"tp_pub_additional_year\">2024<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7293\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7293','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7293\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7293','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7293\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{O&amp;#039;Keefe2024,<br \/>\r\ntitle = {Single-molecule epiallelic profiling of DNA derived from routinely collected Pap specimens for noninvasive detection of ovarian cancer},<br \/>\r\nauthor = {C.M. O&#039;Keefe, Y. Zhao, L.M. Cope, C.M. Ho, A.N. Fader, R. Stone, J.S. Ferris, A. Beavis, K. Levinson, S. Wethington, T.L. Wang, T.R. Pisanic, I.M. Shih, T.H. Wang},<br \/>\r\nurl = {https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ctm2.1778},<br \/>\r\ndoi = {https:\/\/doi.org\/10.1002\/ctm2.1778},<br \/>\r\nyear  = {2024},<br \/>\r\ndate = {2024-07-31},<br \/>\r\njournal = {Clinical and Translational Medicine},<br \/>\r\nvolume = {14},<br \/>\r\nissue = {8},<br \/>\r\npages = {e1778},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7293','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7293\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ctm2.1778\" title=\"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ctm2.1778\" target=\"_blank\">https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ctm2.1778<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/https:\/\/doi.org\/10.1002\/ctm2.1778\" title=\"Follow DOI:https:\/\/doi.org\/10.1002\/ctm2.1778\" target=\"_blank\">doi:https:\/\/doi.org\/10.1002\/ctm2.1778<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7293','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_number\">10.<\/div><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">P. Akarapipad J.S. Park, F. E. Chen<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7295','tp_links')\" style=\"cursor:pointer;\">Digitized Kinetic Analysis Enhances Genotyping Capacity of CRISPR-Based Biosensing<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">ACS Nano, <\/span><span class=\"tp_pub_additional_volume\">vol. 18, <\/span><span class=\"tp_pub_additional_issue\">iss. 27, <\/span><span class=\"tp_pub_additional_year\">2024<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7295\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7295','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7295\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7295','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7295\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{nokey,<br \/>\r\ntitle = {Digitized Kinetic Analysis Enhances Genotyping Capacity of CRISPR-Based Biosensing},<br \/>\r\nauthor = {J.S. Park, P. Akarapipad, F.E. Chen, F. Shao, H. Mostafa, K. Hsieh, T.H. Wang},<br \/>\r\nurl = {https:\/\/pubs.acs.org\/doi\/10.1021\/acsnano.4c05312},<br \/>\r\ndoi = {doi\/10.1021\/acsnano.4c05312.},<br \/>\r\nyear  = {2024},<br \/>\r\ndate = {2024-06-26},<br \/>\r\njournal = {ACS Nano},<br \/>\r\nvolume = {18},<br \/>\r\nissue = {27},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7295','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7295\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/pubs.acs.org\/doi\/10.1021\/acsnano.4c05312\" title=\"https:\/\/pubs.acs.org\/doi\/10.1021\/acsnano.4c05312\" target=\"_blank\">https:\/\/pubs.acs.org\/doi\/10.1021\/acsnano.4c05312<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/doi\/10.1021\/acsnano.4c05312.\" title=\"Follow DOI:doi\/10.1021\/acsnano.4c05312.\" target=\"_blank\">doi:doi\/10.1021\/acsnano.4c05312.<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7295','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_number\">11.<\/div><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">P. Akarapipad H.T. Ngo, P. W. Lee<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7313','tp_links')\" style=\"cursor:pointer;\">Rapid and Portable Quantification of HIV RNA via a Smartphone-enabled Digital CRISPR Device and Deep Learning<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Sensors and Actuators Reports, <\/span><span class=\"tp_pub_additional_volume\">vol. 8, <\/span><span class=\"tp_pub_additional_year\">2024<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7313\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7313','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7313\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7313','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7313\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Ngo2024,<br \/>\r\ntitle = {Rapid and Portable Quantification of HIV RNA via a Smartphone-enabled Digital CRISPR Device and Deep Learning},<br \/>\r\nauthor = {H.T. Ngo, P. Akarapipad, P.W. Lee, J.S.Park, F.E. Chen, A.Y. Trick, K. Hsieh, T.H. Wang},<br \/>\r\nurl = {https:\/\/www.sciencedirect.com\/science\/article\/pii\/S2666053924000286},<br \/>\r\ndoi = {https:\/\/doi.org\/10.1016\/j.snr.2024.100212},<br \/>\r\nyear  = {2024},<br \/>\r\ndate = {2024-06-19},<br \/>\r\njournal = {Sensors and Actuators Reports},<br \/>\r\nvolume = {8},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7313','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7313\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S2666053924000286\" title=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S2666053924000286\" target=\"_blank\">https:\/\/www.sciencedirect.com\/science\/article\/pii\/S2666053924000286<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/https:\/\/doi.org\/10.1016\/j.snr.2024.100212\" title=\"Follow DOI:https:\/\/doi.org\/10.1016\/j.snr.2024.100212\" target=\"_blank\">doi:https:\/\/doi.org\/10.1016\/j.snr.2024.100212<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7313','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_number\">12.<\/div><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">Y Hu S Li, J Lin<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7297','tp_links')\" style=\"cursor:pointer;\">Single-Particle Spectroscopic Chromatography Reveals Heterogeneous RNA Loading and Size Correlations in Lipid Nanoparticles<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">ACS Nano, <\/span><span class=\"tp_pub_additional_volume\">vol. 18, <\/span><span class=\"tp_pub_additional_issue\">iss. 24, <\/span><span class=\"tp_pub_additional_year\">2024<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7297\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7297','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7297\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7297','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7297\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Li2024,<br \/>\r\ntitle = {Single-Particle Spectroscopic Chromatography Reveals Heterogeneous RNA Loading and Size Correlations in Lipid Nanoparticles},<br \/>\r\nauthor = {S Li, Y Hu, J Lin, Z Schneiderman, F Shao, L Wei, A Li, K Hsieh, E Kokkoli, T. Curk, H.Q. Mao, T.H. Wang},<br \/>\r\nurl = {https:\/\/pubs.acs.org\/doi\/full\/10.1021\/acsnano.4c02341},<br \/>\r\ndoi = {doi\/10.1021\/acsnano.4c02341},<br \/>\r\nyear  = {2024},<br \/>\r\ndate = {2024-06-05},<br \/>\r\njournal = {ACS Nano},<br \/>\r\nvolume = {18},<br \/>\r\nissue = {24},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7297','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7297\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/pubs.acs.org\/doi\/full\/10.1021\/acsnano.4c02341\" title=\"https:\/\/pubs.acs.org\/doi\/full\/10.1021\/acsnano.4c02341\" target=\"_blank\">https:\/\/pubs.acs.org\/doi\/full\/10.1021\/acsnano.4c02341<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/doi\/10.1021\/acsnano.4c02341\" title=\"Follow DOI:doi\/10.1021\/acsnano.4c02341\" target=\"_blank\">doi:doi\/10.1021\/acsnano.4c02341<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7297','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_number\">13.<\/div><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">A. Stark A.C. Hasnain, A. Y. Trick<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7299','tp_links')\" style=\"cursor:pointer;\">Cancer Methylation Biomarker Detection in an Automated, Portable, Multichannel Magnetofluidic Platform<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">ACS Nano, <\/span><span class=\"tp_pub_additional_volume\">vol. 18, <\/span><span class=\"tp_pub_additional_issue\">iss. 19, <\/span><span class=\"tp_pub_additional_pages\">pp. 12105\u201312116, <\/span><span class=\"tp_pub_additional_year\">2024<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7299\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7299','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7299\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7299','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7299\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Hasnain2024,<br \/>\r\ntitle = {Cancer Methylation Biomarker Detection in an Automated, Portable, Multichannel Magnetofluidic Platform},<br \/>\r\nauthor = {A.C. Hasnain, A. Stark, A.Y. Trick, K. Ma, K. Hsieh, Y. Cheng, S.J. Meltzer, T.H. Wang},<br \/>\r\nurl = {https:\/\/pubs.acs.org\/doi\/10.1021\/acsnano.3c10070},<br \/>\r\ndoi = {doi\/10.1021\/acsnano.3c10070},<br \/>\r\nyear  = {2024},<br \/>\r\ndate = {2024-04-26},<br \/>\r\njournal = {ACS Nano},<br \/>\r\nvolume = {18},<br \/>\r\nissue = {19},<br \/>\r\npages = {12105\u201312116},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7299','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7299\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/pubs.acs.org\/doi\/10.1021\/acsnano.3c10070\" title=\"https:\/\/pubs.acs.org\/doi\/10.1021\/acsnano.3c10070\" target=\"_blank\">https:\/\/pubs.acs.org\/doi\/10.1021\/acsnano.3c10070<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/doi\/10.1021\/acsnano.3c10070\" title=\"Follow DOI:doi\/10.1021\/acsnano.3c10070\" target=\"_blank\">doi:doi\/10.1021\/acsnano.3c10070<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7299','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_number\">14.<\/div><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">P.W. Lee A. Traylor, K. Hsieh<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7301','tp_links')\" style=\"cursor:pointer;\">Improving bacteria identification from digital melt assay via oligonucleotide-based temperature calibration<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Analytica Chimica Acta, <\/span><span class=\"tp_pub_additional_volume\">vol. 1297, <\/span><span class=\"tp_pub_additional_issue\">iss. 342371, <\/span><span class=\"tp_pub_additional_year\">2024<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7301\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7301','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7301\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7301','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7301\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{nokey,<br \/>\r\ntitle = {Improving bacteria identification from digital melt assay via oligonucleotide-based temperature calibration},<br \/>\r\nauthor = {A. Traylor, P.W. Lee, K. Hsieh, T.H. Wang},<br \/>\r\nurl = {https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0003267024001727},<br \/>\r\ndoi = {https:\/\/doi.org\/10.1016\/j.aca.2024.342371},<br \/>\r\nyear  = {2024},<br \/>\r\ndate = {2024-04-08},<br \/>\r\njournal = {Analytica Chimica Acta},<br \/>\r\nvolume = {1297},<br \/>\r\nissue = {342371},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7301','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7301\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0003267024001727\" title=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0003267024001727\" target=\"_blank\">https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0003267024001727<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/https:\/\/doi.org\/10.1016\/j.aca.2024.342371\" title=\"Follow DOI:https:\/\/doi.org\/10.1016\/j.aca.2024.342371\" target=\"_blank\">doi:https:\/\/doi.org\/10.1016\/j.aca.2024.342371<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7301','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_number\">15.<\/div><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">H. Li F. Shao, K. Hsieh<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7305','tp_links')\" style=\"cursor:pointer;\">Automated and miniaturized screening of antibiotic combinations via robotic-printed combinatorial droplet platform<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Acta Pharmaceutica Sinica B, <\/span><span class=\"tp_pub_additional_volume\">vol. 14, <\/span><span class=\"tp_pub_additional_issue\">iss. 4, <\/span><span class=\"tp_pub_additional_year\">2024<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7305\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7305','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7305\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7305','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7305\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Shao2024,<br \/>\r\ntitle = {Automated and miniaturized screening of antibiotic combinations via robotic-printed combinatorial droplet platform},<br \/>\r\nauthor = {F. Shao, H. Li, K. Hsieh, P. Zhang, S. Li, T.H. Wang},<br \/>\r\nurl = {https:\/\/www.sciencedirect.com\/science\/article\/pii\/S2211383523004501},<br \/>\r\ndoi = {https:\/\/doi.org\/10.1016\/j.apsb.2023.11.027},<br \/>\r\nyear  = {2024},<br \/>\r\ndate = {2024-04-07},<br \/>\r\njournal = {Acta Pharmaceutica Sinica B},<br \/>\r\nvolume = {14},<br \/>\r\nissue = {4},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7305','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7305\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S2211383523004501\" title=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S2211383523004501\" target=\"_blank\">https:\/\/www.sciencedirect.com\/science\/article\/pii\/S2211383523004501<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/https:\/\/doi.org\/10.1016\/j.apsb.2023.11.027\" title=\"Follow DOI:https:\/\/doi.org\/10.1016\/j.apsb.2023.11.027\" target=\"_blank\">doi:https:\/\/doi.org\/10.1016\/j.apsb.2023.11.027<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7305','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_bachelorthesis\"><div class=\"tp_pub_number\">16.<\/div><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">A.Y. Trick M. Jin, M. Totten<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7303','tp_links')\" style=\"cursor:pointer;\">Streamlined instrument-free lysis for the detection of Candida auris<\/a> <span class=\"tp_pub_type tp_  bachelorthesis\">Bachelor Thesis<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_year\">2023<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7303\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7303','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7303\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7303','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7303\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@bachelorthesis{Jin2023,<br \/>\r\ntitle = {Streamlined instrument-free lysis for the detection of Candida auris},<br \/>\r\nauthor = {M. Jin, A.Y. Trick, M. Totten, P. Lee, S.X. Zhang, T.H. Wang},<br \/>\r\nurl = {https:\/\/www.nature.com\/articles\/s41598-023-47220-7},<br \/>\r\ndoi = {https:\/\/doi.org\/10.1038\/s41598-023-47220-7},<br \/>\r\nyear  = {2023},<br \/>\r\ndate = {2023-12-09},<br \/>\r\njournal = {Scientific Reports},<br \/>\r\nvolume = {13},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {bachelorthesis}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7303','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7303\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/www.nature.com\/articles\/s41598-023-47220-7\" title=\"https:\/\/www.nature.com\/articles\/s41598-023-47220-7\" target=\"_blank\">https:\/\/www.nature.com\/articles\/s41598-023-47220-7<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/https:\/\/doi.org\/10.1038\/s41598-023-47220-7\" title=\"Follow DOI:https:\/\/doi.org\/10.1038\/s41598-023-47220-7\" target=\"_blank\">doi:https:\/\/doi.org\/10.1038\/s41598-023-47220-7<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7303','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_number\">17.<\/div><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">W. Gao T. S. Khire, B. Bales<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7307','tp_links')\" style=\"cursor:pointer;\">Rapid Minimum Inhibitory Concentration (MIC) Analysis Using Lyophilized Reagent Beads in a Novel Multiphase, Single-Vessel Assay<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Antibiotics, <\/span><span class=\"tp_pub_additional_volume\">vol. 12, <\/span><span class=\"tp_pub_additional_issue\">iss. 11, <\/span><span class=\"tp_pub_additional_pages\">pp. 1641, <\/span><span class=\"tp_pub_additional_year\">2023<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7307\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7307','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7307\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7307','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7307\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Khire2023,<br \/>\r\ntitle = {Rapid Minimum Inhibitory Concentration (MIC) Analysis Using Lyophilized Reagent Beads in a Novel Multiphase, Single-Vessel Assay},<br \/>\r\nauthor = {T. S. Khire, W. Gao, B. Bales, K. Hsieh, G. Grossmann, D.J.M. Park, C. O\u2019Keefe, A. Brown-Countess, S. Peterson, F.E. Chen, R. Lenigk, A. Trick, T.H. Wang, C. Puleo},<br \/>\r\nurl = {https:\/\/www.mdpi.com\/2079-6382\/12\/11\/1641},<br \/>\r\ndoi = {https:\/\/doi.org\/10.3390\/antibiotics12111641},<br \/>\r\nyear  = {2023},<br \/>\r\ndate = {2023-11-19},<br \/>\r\njournal = {Antibiotics},<br \/>\r\nvolume = {12},<br \/>\r\nissue = {11},<br \/>\r\npages = {1641},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7307','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7307\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/www.mdpi.com\/2079-6382\/12\/11\/1641\" title=\"https:\/\/www.mdpi.com\/2079-6382\/12\/11\/1641\" target=\"_blank\">https:\/\/www.mdpi.com\/2079-6382\/12\/11\/1641<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/https:\/\/doi.org\/10.3390\/antibiotics12111641\" title=\"Follow DOI:https:\/\/doi.org\/10.3390\/antibiotics12111641\" target=\"_blank\">doi:https:\/\/doi.org\/10.3390\/antibiotics12111641<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7307','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_number\">18.<\/div><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">L. Chen P.W. Lee, K. Hsieh<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7309','tp_links')\" style=\"cursor:pointer;\">Harnessing Variabilities in Digital Melt Curves for Accurate Identification of Bacteria<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Analytical Chemistry, <\/span><span class=\"tp_pub_additional_volume\">vol. 95, <\/span><span class=\"tp_pub_additional_issue\">iss. 42, <\/span><span class=\"tp_pub_additional_year\">2023<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7309\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7309','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7309\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7309','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7309\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{nokey,<br \/>\r\ntitle = {Harnessing Variabilities in Digital Melt Curves for Accurate Identification of Bacteria},<br \/>\r\nauthor = {P.W. Lee, L. Chen, K. Hsieh, A. Traylor, T.H. Wang},<br \/>\r\nurl = {https:\/\/pubs.acs.org\/doi\/10.1021\/acs.analchem.3c01654},<br \/>\r\ndoi = {doi\/10.1021\/acs.analchem.3c01654},<br \/>\r\nyear  = {2023},<br \/>\r\ndate = {2023-10-09},<br \/>\r\njournal = {Analytical Chemistry},<br \/>\r\nvolume = {95},<br \/>\r\nissue = {42},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7309','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7309\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/pubs.acs.org\/doi\/10.1021\/acs.analchem.3c01654\" title=\"https:\/\/pubs.acs.org\/doi\/10.1021\/acs.analchem.3c01654\" target=\"_blank\">https:\/\/pubs.acs.org\/doi\/10.1021\/acs.analchem.3c01654<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/doi\/10.1021\/acs.analchem.3c01654\" title=\"Follow DOI:doi\/10.1021\/acs.analchem.3c01654\" target=\"_blank\">doi:doi\/10.1021\/acs.analchem.3c01654<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7309','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_online\"><div class=\"tp_pub_number\">19.<\/div><div class=\"tp_pub_info\"><p class=\"tp_pub_author\"> Ngo, HT.;  Akarapipad, P.;  Lee, PW.;  Park, JS.;  Chen, FE.;  Trick, AY.;  Hsieh, K.;  Wang, TH.<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7254','tp_links')\" style=\"cursor:pointer;\">Rapid and Portable Quantification of HIV RNA via a Smartphone-enabled Digital CRISPR Device and Deep Learning<\/a> <span class=\"tp_pub_type tp_  online\">Online<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_year\">2023<\/span><span class=\"tp_pub_additional_urldate\">, visited: 16.05.2023<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_abstract_link\"><a id=\"tp_abstract_sh_7254\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7254','tp_abstract')\" title=\"Show abstract\" style=\"cursor:pointer;\">Abstract<\/a><\/span> | <span class=\"tp_resource_link\"><a id=\"tp_links_sh_7254\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7254','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7254\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7254','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7254\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@online{Ngo2023,<br \/>\r\ntitle = {Rapid and Portable Quantification of HIV RNA via a Smartphone-enabled Digital CRISPR Device and Deep Learning},<br \/>\r\nauthor = {HT. Ngo and P. Akarapipad and PW. Lee and JS. Park and FE. Chen and AY. Trick and K. Hsieh and TH. Wang},<br \/>\r\nurl = {https:\/\/www.medrxiv.org\/content\/10.1101\/2023.05.12.23289911v1},<br \/>\r\ndoi = {doi: https:\/\/doi.org\/10.1101\/2023.05.12.23289911},<br \/>\r\nyear  = {2023},<br \/>\r\ndate = {2023-05-16},<br \/>\r\nurldate = {2023-05-16},<br \/>\r\nabstract = {For the 28.2 million people in the world living with HIV\/AIDS and receiving antiretroviral therapy, it is crucial to monitor their HIV viral loads with ease. To this end, rapid and portable diagnostic tools that can quantify HIV RNA are critically needed. We report herein a rapid and quantitative digital CRISPR-assisted HIV RNA detection assay that has been implemented within a portable smartphone-based device as a potential solution. Specifically, we first developed a fluorescence-based reverse transcription recombinase polymerase amplification (RT-RPA)-CRISPR assay for isothermally and rapidly detecting HIV RNA at 42 \u00b0C in &lt; 30 min. When realized within a commercial stamp-sized digital chip, this assay yields strongly fluorescent digital reaction wells corresponding to HIV RNA. The isothermal reaction condition and the strong fluorescence in the small digital chip unlock compact thermal and optical components in our device, allowing us to engineer a palm-size (70 \u00d7 115 \u00d7 80 mm) and lightweight (&lt; 0.6 kg) device. Further leveraging the smartphone, we wrote a custom app to control the device, perform the digital assay, and acquire fluorescence images throughout the assay time. We additionally trained and verified a Deep Learning-based algorithm for analyzing fluorescence images and detecting strongly fluorescent digital reaction wells. Using our smartphone-enabled digital CRISPR device, we were able to detect 75 copies of HIV RNA in 15 min and demonstrate the potential of our device toward convenient monitoring of HIV viral loads and combating the HIV\/AIDS epidemic.},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {online}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7254','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_abstract\" id=\"tp_abstract_7254\" style=\"display:none;\"><div class=\"tp_abstract_entry\">For the 28.2 million people in the world living with HIV\/AIDS and receiving antiretroviral therapy, it is crucial to monitor their HIV viral loads with ease. To this end, rapid and portable diagnostic tools that can quantify HIV RNA are critically needed. We report herein a rapid and quantitative digital CRISPR-assisted HIV RNA detection assay that has been implemented within a portable smartphone-based device as a potential solution. Specifically, we first developed a fluorescence-based reverse transcription recombinase polymerase amplification (RT-RPA)-CRISPR assay for isothermally and rapidly detecting HIV RNA at 42 \u00b0C in &lt; 30 min. When realized within a commercial stamp-sized digital chip, this assay yields strongly fluorescent digital reaction wells corresponding to HIV RNA. The isothermal reaction condition and the strong fluorescence in the small digital chip unlock compact thermal and optical components in our device, allowing us to engineer a palm-size (70 \u00d7 115 \u00d7 80 mm) and lightweight (&lt; 0.6 kg) device. Further leveraging the smartphone, we wrote a custom app to control the device, perform the digital assay, and acquire fluorescence images throughout the assay time. We additionally trained and verified a Deep Learning-based algorithm for analyzing fluorescence images and detecting strongly fluorescent digital reaction wells. Using our smartphone-enabled digital CRISPR device, we were able to detect 75 copies of HIV RNA in 15 min and demonstrate the potential of our device toward convenient monitoring of HIV viral loads and combating the HIV\/AIDS epidemic.<\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7254','tp_abstract')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7254\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/www.medrxiv.org\/content\/10.1101\/2023.05.12.23289911v1\" title=\"https:\/\/www.medrxiv.org\/content\/10.1101\/2023.05.12.23289911v1\" target=\"_blank\">https:\/\/www.medrxiv.org\/content\/10.1101\/2023.05.12.23289911v1<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/doi: https:\/\/doi.org\/10.1101\/2023.05.12.23289911\" title=\"Follow DOI:doi: https:\/\/doi.org\/10.1101\/2023.05.12.23289911\" target=\"_blank\">doi:doi: https:\/\/doi.org\/10.1101\/2023.05.12.23289911<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7254','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_bachelorthesis\"><div class=\"tp_pub_number\">20.<\/div><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">N. Ram-Mohan K.C. Tjandra, R. Abe<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7319','tp_links')\" style=\"cursor:pointer;\">Rapid Molecular Phenotypic Antimicrobial Susceptibility Test for Neisseria gonorrhoeae Based on Propidium Monoazide Viability PCR<\/a> <span class=\"tp_pub_type tp_  bachelorthesis\">Bachelor Thesis<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_year\">2023<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7319\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7319','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7319\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7319','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7319\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@bachelorthesis{Tjandra2023b,<br \/>\r\ntitle = {Rapid Molecular Phenotypic Antimicrobial Susceptibility Test for Neisseria gonorrhoeae Based on Propidium Monoazide Viability PCR},<br \/>\r\nauthor = {K.C. Tjandra, N. Ram-Mohan, R. Abe, T.H. Wang, S. Yang},<br \/>\r\nurl = {https:\/\/pubmed.ncbi.nlm.nih.gov\/37115656\/},<br \/>\r\ndoi = {DOI: 10.1021\/acsinfecdis.3c00096},<br \/>\r\nyear  = {2023},<br \/>\r\ndate = {2023-05-12},<br \/>\r\njournal = {ACS Infectious Diseases},<br \/>\r\nvolume = {95},<br \/>\r\nissue = {5},<br \/>\r\npages = {1160-1167},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {bachelorthesis}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7319','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7319\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/37115656\/\" title=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/37115656\/\" target=\"_blank\">https:\/\/pubmed.ncbi.nlm.nih.gov\/37115656\/<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/DOI: 10.1021\/acsinfecdis.3c00096\" title=\"Follow DOI:DOI: 10.1021\/acsinfecdis.3c00096\" target=\"_blank\">doi:DOI: 10.1021\/acsinfecdis.3c00096<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7319','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><\/div><div class=\"tablenav\"><div class=\"tablenav-pages\"><span class=\"displaying-num\">212 entries<\/span> <a class=\"page-numbers button disabled\">&laquo;<\/a> <a class=\"page-numbers button disabled\">&lsaquo;<\/a> 1 of 11 <a href=\"https:\/\/me.jhu.edu\/thwang\/publications.html?limit=2&amp;tgid=&amp;yr=&amp;type=&amp;usr=&amp;auth=&amp;tsr=\" title=\"next page\" class=\"page-numbers button\">&rsaquo;<\/a> <a href=\"https:\/\/me.jhu.edu\/thwang\/publications.html?limit=11&amp;tgid=&amp;yr=&amp;type=&amp;usr=&amp;auth=&amp;tsr=\" title=\"last page\" class=\"page-numbers button\">&raquo;<\/a> <\/div><\/div><\/div><br \/>\n<div class=\"teachpress_pub_list\"><form name=\"tppublistform\" method=\"get\"><a name=\"tppubs\" id=\"tppubs\"><\/a><\/form><div class=\"tablenav\"><div class=\"tablenav-pages\"><span class=\"displaying-num\">212 entries<\/span> <a class=\"page-numbers button disabled\">&laquo;<\/a> <a class=\"page-numbers button disabled\">&lsaquo;<\/a> 1 of 6 <a href=\"https:\/\/me.jhu.edu\/thwang\/publications.html?limit=2&amp;tgid=&amp;yr=&amp;type=&amp;usr=&amp;auth=&amp;tsr=#tppubs\" title=\"next page\" class=\"page-numbers button\">&rsaquo;<\/a> <a href=\"https:\/\/me.jhu.edu\/thwang\/publications.html?limit=6&amp;tgid=&amp;yr=&amp;type=&amp;usr=&amp;auth=&amp;tsr=#tppubs\" title=\"last page\" class=\"page-numbers button\">&raquo;<\/a> <\/div><\/div><div class=\"teachpress_publication_list\"><h3 class=\"tp_h3\" id=\"tp_h3_2025\">2025<\/h3><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">D.J.M. Park, T. Wu, L. Chen, P.W. Lee, K. Hsieh; T.H. Wang<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7280','tp_links')\" style=\"cursor:pointer;\">Simple dual filter workflow for facilitating blood culture-free and sensitive detection of pathogenic bacteria from blood<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Scientific Reports, <\/span><span class=\"tp_pub_additional_volume\">vol. 15, <\/span><span class=\"tp_pub_additional_issue\">iss. 1, <\/span><span class=\"tp_pub_additional_number\">no. 24766, <\/span><span class=\"tp_pub_additional_year\">2025<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7280\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7280','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7280\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7280','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7280\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Park2025,<br \/>\r\ntitle = {Simple dual filter workflow for facilitating blood culture-free and sensitive detection of pathogenic bacteria from blood},<br \/>\r\nauthor = {D.J.M. Park, T. Wu, L. Chen, P.W. Lee, K. Hsieh and T.H. Wang},<br \/>\r\ndoi = {https:\/\/doi.org\/10.1038\/s41598-025-08987-z},<br \/>\r\nyear  = {2025},<br \/>\r\ndate = {2025-07-09},<br \/>\r\nurldate = {2025-07-09},<br \/>\r\njournal = {Scientific Reports},<br \/>\r\nvolume = {15},<br \/>\r\nnumber = {24766},<br \/>\r\nissue = {1},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7280','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7280\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/https:\/\/doi.org\/10.1038\/s41598-025-08987-z\" title=\"Follow DOI:https:\/\/doi.org\/10.1038\/s41598-025-08987-z\" target=\"_blank\">doi:https:\/\/doi.org\/10.1038\/s41598-025-08987-z<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7280','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">J.S. Park, J. Hu, L. Chen,; T.H. Wang<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7281','tp_links')\" style=\"cursor:pointer;\">FlexPCR: A Streamlined Multiplexed Digital mRNA Quantification Platform with Universal Primers and Limited Fluorescence Channels<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Biosensors and Bioelectronics, <\/span><span class=\"tp_pub_additional_volume\">vol. 277, <\/span><span class=\"tp_pub_additional_pages\">pp. 117277, <\/span><span class=\"tp_pub_additional_year\">2025<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7281\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7281','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7281\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7281','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7281\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Park2025b,<br \/>\r\ntitle = {FlexPCR: A Streamlined Multiplexed Digital mRNA Quantification Platform with Universal Primers and Limited Fluorescence Channels},<br \/>\r\nauthor = {J.S. Park, J. Hu, L. Chen, and T.H. Wang},<br \/>\r\nurl = {https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0956566325001514},<br \/>\r\ndoi = {https:\/\/doi.org\/10.1016\/j.bios.2025.117277},<br \/>\r\nyear  = {2025},<br \/>\r\ndate = {2025-06-01},<br \/>\r\njournal = {Biosensors and Bioelectronics},<br \/>\r\nvolume = {277},<br \/>\r\npages = {117277},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7281','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7281\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0956566325001514\" title=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0956566325001514\" target=\"_blank\">https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0956566325001514<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/https:\/\/doi.org\/10.1016\/j.bios.2025.117277\" title=\"Follow DOI:https:\/\/doi.org\/10.1016\/j.bios.2025.117277\" target=\"_blank\">doi:https:\/\/doi.org\/10.1016\/j.bios.2025.117277<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7281','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">P.W. Lee, M. Totten, A. Traylor, S.X. Zhang, T.H. Wang; K. Hsieh<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7287','tp_links')\" style=\"cursor:pointer;\">Cross-Kingdom Pathogen Detection via Duplex Universal PCR and High-Resolution Melt<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Biosensors and Bioelectronics, <\/span><span class=\"tp_pub_additional_volume\">vol. 270, <\/span><span class=\"tp_pub_additional_number\">no. 116922, <\/span><span class=\"tp_pub_additional_year\">2025<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7287\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7287','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7287\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7287','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7287\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Lee2025,<br \/>\r\ntitle = {Cross-Kingdom Pathogen Detection via Duplex Universal PCR and High-Resolution Melt},<br \/>\r\nauthor = {P.W. Lee, M. Totten, A. Traylor, S.X. Zhang, T.H. Wang and K. Hsieh},<br \/>\r\nurl = {https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0956566324009291},<br \/>\r\ndoi = {https:\/\/doi.org\/10.1016\/j.bios.2024.116922},<br \/>\r\nyear  = {2025},<br \/>\r\ndate = {2025-02-15},<br \/>\r\nurldate = {2025-02-15},<br \/>\r\njournal = {Biosensors and Bioelectronics},<br \/>\r\nvolume = {270},<br \/>\r\nnumber = {116922},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7287','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7287\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0956566324009291\" title=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0956566324009291\" target=\"_blank\">https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0956566324009291<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/https:\/\/doi.org\/10.1016\/j.bios.2024.116922\" title=\"Follow DOI:https:\/\/doi.org\/10.1016\/j.bios.2024.116922\" target=\"_blank\">doi:https:\/\/doi.org\/10.1016\/j.bios.2024.116922<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7287','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><h3 class=\"tp_h3\" id=\"tp_h3_2024\">2024<\/h3><div class=\"tp_publication tp_publication_bachelorthesis\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">Y. Zhao, C.M. O&#039;Keefe, K. Hsieh, L. Cope, S.C. Joyce, T.R. Pisanic, J.G. Herman, T.H. Wang<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7311','tp_links')\" style=\"cursor:pointer;\">Multiplex Digital Methylation\u2010Specific PCR for Noninvasive Screening of Lung Cancer<\/a> <span class=\"tp_pub_type tp_  bachelorthesis\">Bachelor Thesis<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_year\">2024<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7311\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7311','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7311\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7311','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7311\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@bachelorthesis{Zhao2024b,<br \/>\r\ntitle = {Multiplex Digital Methylation\u2010Specific PCR for Noninvasive Screening of Lung Cancer},<br \/>\r\nauthor = {Y. Zhao, C.M. O&#039;Keefe, K. Hsieh, L. Cope, S.C. Joyce, T.R. Pisanic, J.G. Herman, T.H. Wang},<br \/>\r\nurl = {https:\/\/www.science.org\/doi\/10.1126\/sciadv.adp1704},<br \/>\r\ndoi = {DOI: 10.1126\/sciadv.adp1704},<br \/>\r\nyear  = {2024},<br \/>\r\ndate = {2024-11-22},<br \/>\r\njournal = {Advanced Science},<br \/>\r\nvolume = {10},<br \/>\r\nissue = {47},<br \/>\r\npages = {2206518},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {bachelorthesis}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7311','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7311\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/www.science.org\/doi\/10.1126\/sciadv.adp1704\" title=\"https:\/\/www.science.org\/doi\/10.1126\/sciadv.adp1704\" target=\"_blank\">https:\/\/www.science.org\/doi\/10.1126\/sciadv.adp1704<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/DOI: 10.1126\/sciadv.adp1704\" title=\"Follow DOI:DOI: 10.1126\/sciadv.adp1704\" target=\"_blank\">doi:DOI: 10.1126\/sciadv.adp1704<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7311','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">Y. Zhao, C.M. O\u2019Keefe, J. Hu, C.M. Allan, W. Cui, H. Lei, A. Chiu, K. Hsieh, S.C. Joyce, J.G. Herman, T.R. Pisanic, T.H. Wang<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7283','tp_links')\" style=\"cursor:pointer;\">Multiplex digital profiling of DNA methylation heterogeneity for sensitive and cost-effective cancer detection in low-volume liquid biopsies<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Science Advances, <\/span><span class=\"tp_pub_additional_volume\">vol. 10, <\/span><span class=\"tp_pub_additional_issue\">iss. 47, <\/span><span class=\"tp_pub_additional_year\">2024<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7283\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7283','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7283\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7283','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7283\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Zhao2024,<br \/>\r\ntitle = {Multiplex digital profiling of DNA methylation heterogeneity for sensitive and cost-effective cancer detection in low-volume liquid biopsies},<br \/>\r\nauthor = {Y. Zhao, C.M. O\u2019Keefe, J. Hu, C.M. Allan, W. Cui, H. Lei, A. Chiu, K. Hsieh, S.C. Joyce, J.G. Herman, T.R. Pisanic, T.H. Wang},<br \/>\r\nurl = {https:\/\/www.science.org\/doi\/10.1126\/sciadv.adp1704},<br \/>\r\ndoi = {DOI: 10.1126\/sciadv.adp1704},<br \/>\r\nyear  = {2024},<br \/>\r\ndate = {2024-11-22},<br \/>\r\njournal = {Science Advances},<br \/>\r\nvolume = {10},<br \/>\r\nissue = {47},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7283','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7283\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/www.science.org\/doi\/10.1126\/sciadv.adp1704\" title=\"https:\/\/www.science.org\/doi\/10.1126\/sciadv.adp1704\" target=\"_blank\">https:\/\/www.science.org\/doi\/10.1126\/sciadv.adp1704<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/DOI: 10.1126\/sciadv.adp1704\" title=\"Follow DOI:DOI: 10.1126\/sciadv.adp1704\" target=\"_blank\">doi:DOI: 10.1126\/sciadv.adp1704<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7283','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">J. Hu, P. Zhang, F. Shao, T.H. Wang<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7285','tp_links')\" style=\"cursor:pointer;\">A streamlined proximity extension assay using POEGMA polymer-coated magnetic beads for enhanced protein detection<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Frontiers in Bioengineering and Biotechnology, <\/span><span class=\"tp_pub_additional_volume\">vol. 12, <\/span><span class=\"tp_pub_additional_number\">no. 1462203, <\/span><span class=\"tp_pub_additional_year\">2024<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7285\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7285','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7285\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7285','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7285\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Hu2024,<br \/>\r\ntitle = {A streamlined proximity extension assay using POEGMA polymer-coated magnetic beads for enhanced protein detection},<br \/>\r\nauthor = {J. Hu, P. Zhang, F. Shao, T.H. Wang},<br \/>\r\nurl = {https:\/\/www.frontiersin.org\/journals\/bioengineering-and-biotechnology\/articles\/10.3389\/fbioe.2024.1462203\/full},<br \/>\r\ndoi = {https:\/\/doi.org\/10.3389\/fbioe.2024.1462203},<br \/>\r\nyear  = {2024},<br \/>\r\ndate = {2024-11-20},<br \/>\r\njournal = {Frontiers in Bioengineering and Biotechnology},<br \/>\r\nvolume = {12},<br \/>\r\nnumber = {1462203},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7285','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7285\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/www.frontiersin.org\/journals\/bioengineering-and-biotechnology\/articles\/10.3389\/fbioe.2024.1462203\/full\" title=\"https:\/\/www.frontiersin.org\/journals\/bioengineering-and-biotechnology\/articles\/1[...]\" target=\"_blank\">https:\/\/www.frontiersin.org\/journals\/bioengineering-and-biotechnology\/articles\/1[...]<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/https:\/\/doi.org\/10.3389\/fbioe.2024.1462203\" title=\"Follow DOI:https:\/\/doi.org\/10.3389\/fbioe.2024.1462203\" target=\"_blank\">doi:https:\/\/doi.org\/10.3389\/fbioe.2024.1462203<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7285','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">J. Hu, L. Chen, P. Zhang, F.E. Chen, H. Li, K. Hsieh, S. Li, J.H. Melendez, T.H. Wang<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7289','tp_links')\" style=\"cursor:pointer;\">Exploiting \u03b2\u2010Lactams\u2010Induced Lysis and DNA Fragmentation for Rapid Molecular Antimicrobial Susceptibility Testing of Neisseria Gonorrhoeae via Dual\u2010Digital PCR<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Advanced Science, <\/span><span class=\"tp_pub_additional_volume\">vol. 11, <\/span><span class=\"tp_pub_additional_issue\">iss. 46, <\/span><span class=\"tp_pub_additional_number\">no. 2405272, <\/span><span class=\"tp_pub_additional_year\">2024<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7289\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7289','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7289\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7289','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7289\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Hu2024b,<br \/>\r\ntitle = {Exploiting \u03b2\u2010Lactams\u2010Induced Lysis and DNA Fragmentation for Rapid Molecular Antimicrobial Susceptibility Testing of Neisseria Gonorrhoeae via Dual\u2010Digital PCR},<br \/>\r\nauthor = {J. Hu, L. Chen, P. Zhang, F.E. Chen, H. Li, K. Hsieh, S. Li, J.H. Melendez, T.H. Wang},<br \/>\r\nurl = {https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/advs.202405272?msockid=3d93250867086f4720e3334666846ee6},<br \/>\r\ndoi = {https:\/\/doi.org\/10.1002\/advs.202405272},<br \/>\r\nyear  = {2024},<br \/>\r\ndate = {2024-10-18},<br \/>\r\njournal = {Advanced Science},<br \/>\r\nvolume = {11},<br \/>\r\nnumber = {2405272},<br \/>\r\nissue = {46},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7289','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7289\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/advs.202405272?msockid=3d93250867086f4720e3334666846ee6\" title=\"https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/advs.202405272?msockid=3d93[...]\" target=\"_blank\">https:\/\/advanced.onlinelibrary.wiley.com\/doi\/10.1002\/advs.202405272?msockid=3d93[...]<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/https:\/\/doi.org\/10.1002\/advs.202405272\" title=\"Follow DOI:https:\/\/doi.org\/10.1002\/advs.202405272\" target=\"_blank\">doi:https:\/\/doi.org\/10.1002\/advs.202405272<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7289','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">Y. Hu, S.Y. Tzeng, L. Cheng, J. Lin, A. Villabona-Rueda, S. Yu, S. Li, Z. Schneiderman, Y. Zhu, J. Ma, D.R. Wilson, S.R. Shannon, T. Warren, Y. Rui, C. Qiu, E.W. Kavanagh, K.M. Luly, Y. Zhang, N. Korinetz, F.R. D\u2019Alessio, T.H. Wang, E. Kokkoli, S.K. Reddy, E. Luijten, J.J. Green, H.Q. Mao<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7291','tp_links')\" style=\"cursor:pointer;\">Supramolecular assembly of polycation\/mRNA nanoparticles and in vivo monocyte programming<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Proceedings of the National Academy of Sciences, <\/span><span class=\"tp_pub_additional_volume\">vol. 27, <\/span><span class=\"tp_pub_additional_issue\">iss. 121, <\/span><span class=\"tp_pub_additional_number\">no. 35, <\/span><span class=\"tp_pub_additional_pages\">pp. e2400194121, <\/span><span class=\"tp_pub_additional_year\">2024<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7291\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7291','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7291\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7291','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7291\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Hu2024c,<br \/>\r\ntitle = {Supramolecular assembly of polycation\/mRNA nanoparticles and in vivo monocyte programming},<br \/>\r\nauthor = {Y. Hu, S.Y. Tzeng, L. Cheng, J. Lin, A. Villabona-Rueda, S. Yu, S. Li, Z. Schneiderman, Y. Zhu, J. Ma, D.R. Wilson, S.R. Shannon, T. Warren, Y. Rui, C. Qiu, E.W. Kavanagh, K.M. Luly, Y. Zhang, N. Korinetz, F.R. D\u2019Alessio, T.H. Wang, E. Kokkoli, S.K. Reddy, E. Luijten, J.J. Green, H.Q. Mao},<br \/>\r\nurl = {https:\/\/pubmed.ncbi.nlm.nih.gov\/39172792\/},<br \/>\r\ndoi = {DOI: 10.1073\/pnas.2400194121},<br \/>\r\nyear  = {2024},<br \/>\r\ndate = {2024-08-22},<br \/>\r\njournal = {Proceedings of the National Academy of Sciences},<br \/>\r\nvolume = {27},<br \/>\r\nnumber = {35},<br \/>\r\nissue = {121},<br \/>\r\npages = {e2400194121},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7291','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7291\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/39172792\/\" title=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/39172792\/\" target=\"_blank\">https:\/\/pubmed.ncbi.nlm.nih.gov\/39172792\/<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/DOI: 10.1073\/pnas.2400194121\" title=\"Follow DOI:DOI: 10.1073\/pnas.2400194121\" target=\"_blank\">doi:DOI: 10.1073\/pnas.2400194121<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7291','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">C.M. O&#039;Keefe, Y. Zhao, L.M. Cope, C.M. Ho, A.N. Fader, R. Stone, J.S. Ferris, A. Beavis, K. Levinson, S. Wethington, T.L. Wang, T.R. Pisanic, I.M. Shih, T.H. Wang<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7293','tp_links')\" style=\"cursor:pointer;\">Single-molecule epiallelic profiling of DNA derived from routinely collected Pap specimens for noninvasive detection of ovarian cancer<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Clinical and Translational Medicine, <\/span><span class=\"tp_pub_additional_volume\">vol. 14, <\/span><span class=\"tp_pub_additional_issue\">iss. 8, <\/span><span class=\"tp_pub_additional_pages\">pp. e1778, <\/span><span class=\"tp_pub_additional_year\">2024<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7293\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7293','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7293\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7293','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7293\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{O&amp;#039;Keefe2024,<br \/>\r\ntitle = {Single-molecule epiallelic profiling of DNA derived from routinely collected Pap specimens for noninvasive detection of ovarian cancer},<br \/>\r\nauthor = {C.M. O&#039;Keefe, Y. Zhao, L.M. Cope, C.M. Ho, A.N. Fader, R. Stone, J.S. Ferris, A. Beavis, K. Levinson, S. Wethington, T.L. Wang, T.R. Pisanic, I.M. Shih, T.H. Wang},<br \/>\r\nurl = {https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ctm2.1778},<br \/>\r\ndoi = {https:\/\/doi.org\/10.1002\/ctm2.1778},<br \/>\r\nyear  = {2024},<br \/>\r\ndate = {2024-07-31},<br \/>\r\njournal = {Clinical and Translational Medicine},<br \/>\r\nvolume = {14},<br \/>\r\nissue = {8},<br \/>\r\npages = {e1778},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7293','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7293\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ctm2.1778\" title=\"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ctm2.1778\" target=\"_blank\">https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ctm2.1778<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/https:\/\/doi.org\/10.1002\/ctm2.1778\" title=\"Follow DOI:https:\/\/doi.org\/10.1002\/ctm2.1778\" target=\"_blank\">doi:https:\/\/doi.org\/10.1002\/ctm2.1778<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7293','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">J.S. Park, P. Akarapipad, F.E. Chen, F. Shao, H. Mostafa, K. Hsieh, T.H. Wang<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7295','tp_links')\" style=\"cursor:pointer;\">Digitized Kinetic Analysis Enhances Genotyping Capacity of CRISPR-Based Biosensing<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">ACS Nano, <\/span><span class=\"tp_pub_additional_volume\">vol. 18, <\/span><span class=\"tp_pub_additional_issue\">iss. 27, <\/span><span class=\"tp_pub_additional_year\">2024<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7295\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7295','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7295\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7295','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7295\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{nokey,<br \/>\r\ntitle = {Digitized Kinetic Analysis Enhances Genotyping Capacity of CRISPR-Based Biosensing},<br \/>\r\nauthor = {J.S. Park, P. Akarapipad, F.E. Chen, F. Shao, H. Mostafa, K. Hsieh, T.H. Wang},<br \/>\r\nurl = {https:\/\/pubs.acs.org\/doi\/10.1021\/acsnano.4c05312},<br \/>\r\ndoi = {doi\/10.1021\/acsnano.4c05312.},<br \/>\r\nyear  = {2024},<br \/>\r\ndate = {2024-06-26},<br \/>\r\njournal = {ACS Nano},<br \/>\r\nvolume = {18},<br \/>\r\nissue = {27},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7295','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7295\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/pubs.acs.org\/doi\/10.1021\/acsnano.4c05312\" title=\"https:\/\/pubs.acs.org\/doi\/10.1021\/acsnano.4c05312\" target=\"_blank\">https:\/\/pubs.acs.org\/doi\/10.1021\/acsnano.4c05312<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/doi\/10.1021\/acsnano.4c05312.\" title=\"Follow DOI:doi\/10.1021\/acsnano.4c05312.\" target=\"_blank\">doi:doi\/10.1021\/acsnano.4c05312.<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7295','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">H.T. Ngo, P. Akarapipad, P.W. Lee, J.S.Park, F.E. Chen, A.Y. Trick, K. Hsieh, T.H. Wang<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7313','tp_links')\" style=\"cursor:pointer;\">Rapid and Portable Quantification of HIV RNA via a Smartphone-enabled Digital CRISPR Device and Deep Learning<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Sensors and Actuators Reports, <\/span><span class=\"tp_pub_additional_volume\">vol. 8, <\/span><span class=\"tp_pub_additional_year\">2024<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7313\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7313','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7313\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7313','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7313\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Ngo2024,<br \/>\r\ntitle = {Rapid and Portable Quantification of HIV RNA via a Smartphone-enabled Digital CRISPR Device and Deep Learning},<br \/>\r\nauthor = {H.T. Ngo, P. Akarapipad, P.W. Lee, J.S.Park, F.E. Chen, A.Y. Trick, K. Hsieh, T.H. Wang},<br \/>\r\nurl = {https:\/\/www.sciencedirect.com\/science\/article\/pii\/S2666053924000286},<br \/>\r\ndoi = {https:\/\/doi.org\/10.1016\/j.snr.2024.100212},<br \/>\r\nyear  = {2024},<br \/>\r\ndate = {2024-06-19},<br \/>\r\njournal = {Sensors and Actuators Reports},<br \/>\r\nvolume = {8},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7313','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7313\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S2666053924000286\" title=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S2666053924000286\" target=\"_blank\">https:\/\/www.sciencedirect.com\/science\/article\/pii\/S2666053924000286<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/https:\/\/doi.org\/10.1016\/j.snr.2024.100212\" title=\"Follow DOI:https:\/\/doi.org\/10.1016\/j.snr.2024.100212\" target=\"_blank\">doi:https:\/\/doi.org\/10.1016\/j.snr.2024.100212<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7313','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">S Li, Y Hu, J Lin, Z Schneiderman, F Shao, L Wei, A Li, K Hsieh, E Kokkoli, T. Curk, H.Q. Mao, T.H. Wang<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7297','tp_links')\" style=\"cursor:pointer;\">Single-Particle Spectroscopic Chromatography Reveals Heterogeneous RNA Loading and Size Correlations in Lipid Nanoparticles<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">ACS Nano, <\/span><span class=\"tp_pub_additional_volume\">vol. 18, <\/span><span class=\"tp_pub_additional_issue\">iss. 24, <\/span><span class=\"tp_pub_additional_year\">2024<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7297\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7297','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7297\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7297','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7297\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Li2024,<br \/>\r\ntitle = {Single-Particle Spectroscopic Chromatography Reveals Heterogeneous RNA Loading and Size Correlations in Lipid Nanoparticles},<br \/>\r\nauthor = {S Li, Y Hu, J Lin, Z Schneiderman, F Shao, L Wei, A Li, K Hsieh, E Kokkoli, T. Curk, H.Q. Mao, T.H. Wang},<br \/>\r\nurl = {https:\/\/pubs.acs.org\/doi\/full\/10.1021\/acsnano.4c02341},<br \/>\r\ndoi = {doi\/10.1021\/acsnano.4c02341},<br \/>\r\nyear  = {2024},<br \/>\r\ndate = {2024-06-05},<br \/>\r\njournal = {ACS Nano},<br \/>\r\nvolume = {18},<br \/>\r\nissue = {24},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7297','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7297\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/pubs.acs.org\/doi\/full\/10.1021\/acsnano.4c02341\" title=\"https:\/\/pubs.acs.org\/doi\/full\/10.1021\/acsnano.4c02341\" target=\"_blank\">https:\/\/pubs.acs.org\/doi\/full\/10.1021\/acsnano.4c02341<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/doi\/10.1021\/acsnano.4c02341\" title=\"Follow DOI:doi\/10.1021\/acsnano.4c02341\" target=\"_blank\">doi:doi\/10.1021\/acsnano.4c02341<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7297','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">A.C. Hasnain, A. Stark, A.Y. Trick, K. Ma, K. Hsieh, Y. Cheng, S.J. Meltzer, T.H. Wang<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7299','tp_links')\" style=\"cursor:pointer;\">Cancer Methylation Biomarker Detection in an Automated, Portable, Multichannel Magnetofluidic Platform<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">ACS Nano, <\/span><span class=\"tp_pub_additional_volume\">vol. 18, <\/span><span class=\"tp_pub_additional_issue\">iss. 19, <\/span><span class=\"tp_pub_additional_pages\">pp. 12105\u201312116, <\/span><span class=\"tp_pub_additional_year\">2024<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7299\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7299','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7299\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7299','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7299\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Hasnain2024,<br \/>\r\ntitle = {Cancer Methylation Biomarker Detection in an Automated, Portable, Multichannel Magnetofluidic Platform},<br \/>\r\nauthor = {A.C. Hasnain, A. Stark, A.Y. Trick, K. Ma, K. Hsieh, Y. Cheng, S.J. Meltzer, T.H. Wang},<br \/>\r\nurl = {https:\/\/pubs.acs.org\/doi\/10.1021\/acsnano.3c10070},<br \/>\r\ndoi = {doi\/10.1021\/acsnano.3c10070},<br \/>\r\nyear  = {2024},<br \/>\r\ndate = {2024-04-26},<br \/>\r\njournal = {ACS Nano},<br \/>\r\nvolume = {18},<br \/>\r\nissue = {19},<br \/>\r\npages = {12105\u201312116},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7299','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7299\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/pubs.acs.org\/doi\/10.1021\/acsnano.3c10070\" title=\"https:\/\/pubs.acs.org\/doi\/10.1021\/acsnano.3c10070\" target=\"_blank\">https:\/\/pubs.acs.org\/doi\/10.1021\/acsnano.3c10070<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/doi\/10.1021\/acsnano.3c10070\" title=\"Follow DOI:doi\/10.1021\/acsnano.3c10070\" target=\"_blank\">doi:doi\/10.1021\/acsnano.3c10070<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7299','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">A. Traylor, P.W. Lee, K. Hsieh, T.H. Wang<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7301','tp_links')\" style=\"cursor:pointer;\">Improving bacteria identification from digital melt assay via oligonucleotide-based temperature calibration<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Analytica Chimica Acta, <\/span><span class=\"tp_pub_additional_volume\">vol. 1297, <\/span><span class=\"tp_pub_additional_issue\">iss. 342371, <\/span><span class=\"tp_pub_additional_year\">2024<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7301\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7301','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7301\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7301','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7301\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{nokey,<br \/>\r\ntitle = {Improving bacteria identification from digital melt assay via oligonucleotide-based temperature calibration},<br \/>\r\nauthor = {A. Traylor, P.W. Lee, K. Hsieh, T.H. Wang},<br \/>\r\nurl = {https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0003267024001727},<br \/>\r\ndoi = {https:\/\/doi.org\/10.1016\/j.aca.2024.342371},<br \/>\r\nyear  = {2024},<br \/>\r\ndate = {2024-04-08},<br \/>\r\njournal = {Analytica Chimica Acta},<br \/>\r\nvolume = {1297},<br \/>\r\nissue = {342371},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7301','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7301\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0003267024001727\" title=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0003267024001727\" target=\"_blank\">https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0003267024001727<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/https:\/\/doi.org\/10.1016\/j.aca.2024.342371\" title=\"Follow DOI:https:\/\/doi.org\/10.1016\/j.aca.2024.342371\" target=\"_blank\">doi:https:\/\/doi.org\/10.1016\/j.aca.2024.342371<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7301','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">F. Shao, H. Li, K. Hsieh, P. Zhang, S. Li, T.H. Wang<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7305','tp_links')\" style=\"cursor:pointer;\">Automated and miniaturized screening of antibiotic combinations via robotic-printed combinatorial droplet platform<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Acta Pharmaceutica Sinica B, <\/span><span class=\"tp_pub_additional_volume\">vol. 14, <\/span><span class=\"tp_pub_additional_issue\">iss. 4, <\/span><span class=\"tp_pub_additional_year\">2024<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7305\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7305','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7305\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7305','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7305\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Shao2024,<br \/>\r\ntitle = {Automated and miniaturized screening of antibiotic combinations via robotic-printed combinatorial droplet platform},<br \/>\r\nauthor = {F. Shao, H. Li, K. Hsieh, P. Zhang, S. Li, T.H. Wang},<br \/>\r\nurl = {https:\/\/www.sciencedirect.com\/science\/article\/pii\/S2211383523004501},<br \/>\r\ndoi = {https:\/\/doi.org\/10.1016\/j.apsb.2023.11.027},<br \/>\r\nyear  = {2024},<br \/>\r\ndate = {2024-04-07},<br \/>\r\njournal = {Acta Pharmaceutica Sinica B},<br \/>\r\nvolume = {14},<br \/>\r\nissue = {4},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7305','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7305\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S2211383523004501\" title=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S2211383523004501\" target=\"_blank\">https:\/\/www.sciencedirect.com\/science\/article\/pii\/S2211383523004501<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/https:\/\/doi.org\/10.1016\/j.apsb.2023.11.027\" title=\"Follow DOI:https:\/\/doi.org\/10.1016\/j.apsb.2023.11.027\" target=\"_blank\">doi:https:\/\/doi.org\/10.1016\/j.apsb.2023.11.027<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7305','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><h3 class=\"tp_h3\" id=\"tp_h3_2023\">2023<\/h3><div class=\"tp_publication tp_publication_bachelorthesis\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">M. Jin, A.Y. Trick, M. Totten, P. Lee, S.X. Zhang, T.H. Wang<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7303','tp_links')\" style=\"cursor:pointer;\">Streamlined instrument-free lysis for the detection of Candida auris<\/a> <span class=\"tp_pub_type tp_  bachelorthesis\">Bachelor Thesis<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_year\">2023<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7303\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7303','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7303\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7303','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7303\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@bachelorthesis{Jin2023,<br \/>\r\ntitle = {Streamlined instrument-free lysis for the detection of Candida auris},<br \/>\r\nauthor = {M. Jin, A.Y. Trick, M. Totten, P. Lee, S.X. Zhang, T.H. Wang},<br \/>\r\nurl = {https:\/\/www.nature.com\/articles\/s41598-023-47220-7},<br \/>\r\ndoi = {https:\/\/doi.org\/10.1038\/s41598-023-47220-7},<br \/>\r\nyear  = {2023},<br \/>\r\ndate = {2023-12-09},<br \/>\r\njournal = {Scientific Reports},<br \/>\r\nvolume = {13},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {bachelorthesis}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7303','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7303\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/www.nature.com\/articles\/s41598-023-47220-7\" title=\"https:\/\/www.nature.com\/articles\/s41598-023-47220-7\" target=\"_blank\">https:\/\/www.nature.com\/articles\/s41598-023-47220-7<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/https:\/\/doi.org\/10.1038\/s41598-023-47220-7\" title=\"Follow DOI:https:\/\/doi.org\/10.1038\/s41598-023-47220-7\" target=\"_blank\">doi:https:\/\/doi.org\/10.1038\/s41598-023-47220-7<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7303','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">T. S. Khire, W. Gao, B. Bales, K. Hsieh, G. Grossmann, D.J.M. Park, C. O\u2019Keefe, A. Brown-Countess, S. Peterson, F.E. Chen, R. Lenigk, A. Trick, T.H. Wang, C. Puleo<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7307','tp_links')\" style=\"cursor:pointer;\">Rapid Minimum Inhibitory Concentration (MIC) Analysis Using Lyophilized Reagent Beads in a Novel Multiphase, Single-Vessel Assay<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Antibiotics, <\/span><span class=\"tp_pub_additional_volume\">vol. 12, <\/span><span class=\"tp_pub_additional_issue\">iss. 11, <\/span><span class=\"tp_pub_additional_pages\">pp. 1641, <\/span><span class=\"tp_pub_additional_year\">2023<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7307\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7307','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7307\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7307','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7307\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Khire2023,<br \/>\r\ntitle = {Rapid Minimum Inhibitory Concentration (MIC) Analysis Using Lyophilized Reagent Beads in a Novel Multiphase, Single-Vessel Assay},<br \/>\r\nauthor = {T. S. Khire, W. Gao, B. Bales, K. Hsieh, G. Grossmann, D.J.M. Park, C. O\u2019Keefe, A. Brown-Countess, S. Peterson, F.E. Chen, R. Lenigk, A. Trick, T.H. Wang, C. Puleo},<br \/>\r\nurl = {https:\/\/www.mdpi.com\/2079-6382\/12\/11\/1641},<br \/>\r\ndoi = {https:\/\/doi.org\/10.3390\/antibiotics12111641},<br \/>\r\nyear  = {2023},<br \/>\r\ndate = {2023-11-19},<br \/>\r\njournal = {Antibiotics},<br \/>\r\nvolume = {12},<br \/>\r\nissue = {11},<br \/>\r\npages = {1641},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7307','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7307\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/www.mdpi.com\/2079-6382\/12\/11\/1641\" title=\"https:\/\/www.mdpi.com\/2079-6382\/12\/11\/1641\" target=\"_blank\">https:\/\/www.mdpi.com\/2079-6382\/12\/11\/1641<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/https:\/\/doi.org\/10.3390\/antibiotics12111641\" title=\"Follow DOI:https:\/\/doi.org\/10.3390\/antibiotics12111641\" target=\"_blank\">doi:https:\/\/doi.org\/10.3390\/antibiotics12111641<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7307','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">P.W. Lee, L. Chen, K. Hsieh, A. Traylor, T.H. Wang<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7309','tp_links')\" style=\"cursor:pointer;\">Harnessing Variabilities in Digital Melt Curves for Accurate Identification of Bacteria<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Analytical Chemistry, <\/span><span class=\"tp_pub_additional_volume\">vol. 95, <\/span><span class=\"tp_pub_additional_issue\">iss. 42, <\/span><span class=\"tp_pub_additional_year\">2023<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7309\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7309','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7309\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7309','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7309\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{nokey,<br \/>\r\ntitle = {Harnessing Variabilities in Digital Melt Curves for Accurate Identification of Bacteria},<br \/>\r\nauthor = {P.W. Lee, L. Chen, K. Hsieh, A. Traylor, T.H. Wang},<br \/>\r\nurl = {https:\/\/pubs.acs.org\/doi\/10.1021\/acs.analchem.3c01654},<br \/>\r\ndoi = {doi\/10.1021\/acs.analchem.3c01654},<br \/>\r\nyear  = {2023},<br \/>\r\ndate = {2023-10-09},<br \/>\r\njournal = {Analytical Chemistry},<br \/>\r\nvolume = {95},<br \/>\r\nissue = {42},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7309','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7309\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/pubs.acs.org\/doi\/10.1021\/acs.analchem.3c01654\" title=\"https:\/\/pubs.acs.org\/doi\/10.1021\/acs.analchem.3c01654\" target=\"_blank\">https:\/\/pubs.acs.org\/doi\/10.1021\/acs.analchem.3c01654<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/doi\/10.1021\/acs.analchem.3c01654\" title=\"Follow DOI:doi\/10.1021\/acs.analchem.3c01654\" target=\"_blank\">doi:doi\/10.1021\/acs.analchem.3c01654<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7309','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_online\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">HT. Ngo; P. Akarapipad; PW. Lee; JS. Park; FE. Chen; AY. Trick; K. Hsieh; TH. Wang<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7254','tp_links')\" style=\"cursor:pointer;\">Rapid and Portable Quantification of HIV RNA via a Smartphone-enabled Digital CRISPR Device and Deep Learning<\/a> <span class=\"tp_pub_type tp_  online\">Online<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_year\">2023<\/span><span class=\"tp_pub_additional_urldate\">, visited: 16.05.2023<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_abstract_link\"><a id=\"tp_abstract_sh_7254\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7254','tp_abstract')\" title=\"Show abstract\" style=\"cursor:pointer;\">Abstract<\/a><\/span> | <span class=\"tp_resource_link\"><a id=\"tp_links_sh_7254\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7254','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7254\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7254','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7254\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@online{Ngo2023,<br \/>\r\ntitle = {Rapid and Portable Quantification of HIV RNA via a Smartphone-enabled Digital CRISPR Device and Deep Learning},<br \/>\r\nauthor = {HT. Ngo and P. Akarapipad and PW. Lee and JS. Park and FE. Chen and AY. Trick and K. Hsieh and TH. Wang},<br \/>\r\nurl = {https:\/\/www.medrxiv.org\/content\/10.1101\/2023.05.12.23289911v1},<br \/>\r\ndoi = {doi: https:\/\/doi.org\/10.1101\/2023.05.12.23289911},<br \/>\r\nyear  = {2023},<br \/>\r\ndate = {2023-05-16},<br \/>\r\nurldate = {2023-05-16},<br \/>\r\nabstract = {For the 28.2 million people in the world living with HIV\/AIDS and receiving antiretroviral therapy, it is crucial to monitor their HIV viral loads with ease. To this end, rapid and portable diagnostic tools that can quantify HIV RNA are critically needed. We report herein a rapid and quantitative digital CRISPR-assisted HIV RNA detection assay that has been implemented within a portable smartphone-based device as a potential solution. Specifically, we first developed a fluorescence-based reverse transcription recombinase polymerase amplification (RT-RPA)-CRISPR assay for isothermally and rapidly detecting HIV RNA at 42 \u00b0C in &lt; 30 min. When realized within a commercial stamp-sized digital chip, this assay yields strongly fluorescent digital reaction wells corresponding to HIV RNA. The isothermal reaction condition and the strong fluorescence in the small digital chip unlock compact thermal and optical components in our device, allowing us to engineer a palm-size (70 \u00d7 115 \u00d7 80 mm) and lightweight (&lt; 0.6 kg) device. Further leveraging the smartphone, we wrote a custom app to control the device, perform the digital assay, and acquire fluorescence images throughout the assay time. We additionally trained and verified a Deep Learning-based algorithm for analyzing fluorescence images and detecting strongly fluorescent digital reaction wells. Using our smartphone-enabled digital CRISPR device, we were able to detect 75 copies of HIV RNA in 15 min and demonstrate the potential of our device toward convenient monitoring of HIV viral loads and combating the HIV\/AIDS epidemic.},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {online}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7254','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_abstract\" id=\"tp_abstract_7254\" style=\"display:none;\"><div class=\"tp_abstract_entry\">For the 28.2 million people in the world living with HIV\/AIDS and receiving antiretroviral therapy, it is crucial to monitor their HIV viral loads with ease. To this end, rapid and portable diagnostic tools that can quantify HIV RNA are critically needed. We report herein a rapid and quantitative digital CRISPR-assisted HIV RNA detection assay that has been implemented within a portable smartphone-based device as a potential solution. Specifically, we first developed a fluorescence-based reverse transcription recombinase polymerase amplification (RT-RPA)-CRISPR assay for isothermally and rapidly detecting HIV RNA at 42 \u00b0C in &lt; 30 min. When realized within a commercial stamp-sized digital chip, this assay yields strongly fluorescent digital reaction wells corresponding to HIV RNA. The isothermal reaction condition and the strong fluorescence in the small digital chip unlock compact thermal and optical components in our device, allowing us to engineer a palm-size (70 \u00d7 115 \u00d7 80 mm) and lightweight (&lt; 0.6 kg) device. Further leveraging the smartphone, we wrote a custom app to control the device, perform the digital assay, and acquire fluorescence images throughout the assay time. We additionally trained and verified a Deep Learning-based algorithm for analyzing fluorescence images and detecting strongly fluorescent digital reaction wells. Using our smartphone-enabled digital CRISPR device, we were able to detect 75 copies of HIV RNA in 15 min and demonstrate the potential of our device toward convenient monitoring of HIV viral loads and combating the HIV\/AIDS epidemic.<\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7254','tp_abstract')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7254\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/www.medrxiv.org\/content\/10.1101\/2023.05.12.23289911v1\" title=\"https:\/\/www.medrxiv.org\/content\/10.1101\/2023.05.12.23289911v1\" target=\"_blank\">https:\/\/www.medrxiv.org\/content\/10.1101\/2023.05.12.23289911v1<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/doi: https:\/\/doi.org\/10.1101\/2023.05.12.23289911\" title=\"Follow DOI:doi: https:\/\/doi.org\/10.1101\/2023.05.12.23289911\" target=\"_blank\">doi:doi: https:\/\/doi.org\/10.1101\/2023.05.12.23289911<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7254','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_bachelorthesis\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">K.C. Tjandra, N. Ram-Mohan, R. Abe, T.H. Wang, S. Yang<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7319','tp_links')\" style=\"cursor:pointer;\">Rapid Molecular Phenotypic Antimicrobial Susceptibility Test for Neisseria gonorrhoeae Based on Propidium Monoazide Viability PCR<\/a> <span class=\"tp_pub_type tp_  bachelorthesis\">Bachelor Thesis<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_year\">2023<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7319\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7319','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7319\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7319','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7319\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@bachelorthesis{Tjandra2023b,<br \/>\r\ntitle = {Rapid Molecular Phenotypic Antimicrobial Susceptibility Test for Neisseria gonorrhoeae Based on Propidium Monoazide Viability PCR},<br \/>\r\nauthor = {K.C. Tjandra, N. Ram-Mohan, R. Abe, T.H. Wang, S. Yang},<br \/>\r\nurl = {https:\/\/pubmed.ncbi.nlm.nih.gov\/37115656\/},<br \/>\r\ndoi = {DOI: 10.1021\/acsinfecdis.3c00096},<br \/>\r\nyear  = {2023},<br \/>\r\ndate = {2023-05-12},<br \/>\r\njournal = {ACS Infectious Diseases},<br \/>\r\nvolume = {95},<br \/>\r\nissue = {5},<br \/>\r\npages = {1160-1167},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {bachelorthesis}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7319','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7319\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/37115656\/\" title=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/37115656\/\" target=\"_blank\">https:\/\/pubmed.ncbi.nlm.nih.gov\/37115656\/<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/DOI: 10.1021\/acsinfecdis.3c00096\" title=\"Follow DOI:DOI: 10.1021\/acsinfecdis.3c00096\" target=\"_blank\">doi:DOI: 10.1021\/acsinfecdis.3c00096<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7319','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">KC. Tjandra; N. Ram-Mohan; R. Abe; TH. Wang; S. Yang<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7262','tp_links')\" style=\"cursor:pointer;\">Rapid Molecular Phenotypic Antimicrobial Susceptibility Test for Neisseria gonorrhoeae Based on Propidium Monoazide Viability PCR<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">ACS Infectious Diseases, <\/span><span class=\"tp_pub_additional_volume\">vol. 9, <\/span><span class=\"tp_pub_additional_issue\">iss. 5, <\/span><span class=\"tp_pub_additional_pages\">pp. 1160-1167, <\/span><span class=\"tp_pub_additional_year\">2023<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_abstract_link\"><a id=\"tp_abstract_sh_7262\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7262','tp_abstract')\" title=\"Show abstract\" style=\"cursor:pointer;\">Abstract<\/a><\/span> | <span class=\"tp_resource_link\"><a id=\"tp_links_sh_7262\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7262','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7262\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7262','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7262\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Tjandra2023,<br \/>\r\ntitle = {Rapid Molecular Phenotypic Antimicrobial Susceptibility Test for Neisseria gonorrhoeae Based on Propidium Monoazide Viability PCR},<br \/>\r\nauthor = {KC. Tjandra and N. Ram-Mohan and R. Abe and TH. Wang and S. Yang},<br \/>\r\nurl = {https:\/\/pubmed.ncbi.nlm.nih.gov\/37115656\/},<br \/>\r\ndoi = {doi: 10.1021\/acsinfecdis.3c00096.},<br \/>\r\nyear  = {2023},<br \/>\r\ndate = {2023-05-12},<br \/>\r\njournal = {ACS Infectious Diseases},<br \/>\r\nvolume = {9},<br \/>\r\nissue = {5},<br \/>\r\npages = {1160-1167},<br \/>\r\nabstract = {Neisseria gonorrhoeae (NG) is an urgent threat to antimicrobial resistance (AMR) worldwide. NG has acquired rapid resistance to all previously recommended treatments, leaving ceftriaxone monotherapy as the first and last line of therapy for uncomplicated NG. The ability to rapidly determine susceptibility, which is currently nonexistent for NG, has been proposed as a strategy to preserve ceftriaxone by using alternative treatments. Herein, we used a DNA-intercalating dye in combination with NG-specific primers\/probes to generate qPCR cycle threshold (Ct) values at different concentrations of 2 NG-relevant antimicrobials. Our proof-of-concept dual-antimicrobial logistic regression model based on the differential Ct measurements achieved an AUC of 0.93 with a categorical agreement for the susceptibility of 84.6%. When surveying the performance against each antimicrobial separately, the model predicted 90 and 75% susceptible and resistant strains, respectively, to ceftriaxone and 66.7 and 83.3% susceptible and resistant strains, respectively, to ciprofloxacin. We further validated the model against the individual replicates and determined the accuracy of the model in classifying susceptibility agnostic of the inoculum size. We demonstrated a novel PCR-based approach to determine phenotypic ciprofloxacin and ceftriaxone susceptibility information for NG with reasonable accuracy within 30 min, a significant improvement compared to the conventional method which could take multiple days.},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7262','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_abstract\" id=\"tp_abstract_7262\" style=\"display:none;\"><div class=\"tp_abstract_entry\">Neisseria gonorrhoeae (NG) is an urgent threat to antimicrobial resistance (AMR) worldwide. NG has acquired rapid resistance to all previously recommended treatments, leaving ceftriaxone monotherapy as the first and last line of therapy for uncomplicated NG. The ability to rapidly determine susceptibility, which is currently nonexistent for NG, has been proposed as a strategy to preserve ceftriaxone by using alternative treatments. Herein, we used a DNA-intercalating dye in combination with NG-specific primers\/probes to generate qPCR cycle threshold (Ct) values at different concentrations of 2 NG-relevant antimicrobials. Our proof-of-concept dual-antimicrobial logistic regression model based on the differential Ct measurements achieved an AUC of 0.93 with a categorical agreement for the susceptibility of 84.6%. When surveying the performance against each antimicrobial separately, the model predicted 90 and 75% susceptible and resistant strains, respectively, to ceftriaxone and 66.7 and 83.3% susceptible and resistant strains, respectively, to ciprofloxacin. We further validated the model against the individual replicates and determined the accuracy of the model in classifying susceptibility agnostic of the inoculum size. We demonstrated a novel PCR-based approach to determine phenotypic ciprofloxacin and ceftriaxone susceptibility information for NG with reasonable accuracy within 30 min, a significant improvement compared to the conventional method which could take multiple days.<\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7262','tp_abstract')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7262\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/37115656\/\" title=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/37115656\/\" target=\"_blank\">https:\/\/pubmed.ncbi.nlm.nih.gov\/37115656\/<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/doi: 10.1021\/acsinfecdis.3c00096.\" title=\"Follow DOI:doi: 10.1021\/acsinfecdis.3c00096.\" target=\"_blank\">doi:doi: 10.1021\/acsinfecdis.3c00096.<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7262','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">F. Chen; J. Wang; AH. Nambiar; J. Hardick; J. Melendez; AY. Trick; TH. Wang<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7258','tp_links')\" style=\"cursor:pointer;\">Point-of-Care Amenable Detection of Mycoplasma genitalium and Its Antibiotic Resistance Mutations<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">ACS Sensors, <\/span><span class=\"tp_pub_additional_volume\">vol. 8, <\/span><span class=\"tp_pub_additional_issue\">iss. 4, <\/span><span class=\"tp_pub_additional_pages\">pp. 1550-1557, <\/span><span class=\"tp_pub_additional_year\">2023<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_abstract_link\"><a id=\"tp_abstract_sh_7258\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7258','tp_abstract')\" title=\"Show abstract\" style=\"cursor:pointer;\">Abstract<\/a><\/span> | <span class=\"tp_resource_link\"><a id=\"tp_links_sh_7258\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7258','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7258\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7258','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7258\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Chen2023,<br \/>\r\ntitle = {Point-of-Care Amenable Detection of Mycoplasma genitalium and Its Antibiotic Resistance Mutations},<br \/>\r\nauthor = {F. Chen and J. Wang and AH. Nambiar and J. Hardick and J. Melendez and AY. Trick and TH. Wang},<br \/>\r\nurl = {https:\/\/pubmed.ncbi.nlm.nih.gov\/36961769\/},<br \/>\r\ndoi = {DOI: 10.1021\/acssensors.2c02630},<br \/>\r\nyear  = {2023},<br \/>\r\ndate = {2023-04-28},<br \/>\r\njournal = {ACS Sensors},<br \/>\r\nvolume = {8},<br \/>\r\nissue = {4},<br \/>\r\npages = {1550-1557},<br \/>\r\nabstract = {Mycoplasma genitalium (MG) is an emerging sexually transmitted bacterium. Due to its fastidious and slow-growing nature, MG is difficult to detect through culture-based diagnostics. Like Neisseria gonorrheae, another bacterial pathogen linked to sexually transmitted infections (STIs), MG has developed resistance to macrolide and fluoroquinolone antibiotics used to treat STIs. The ability to detect MG and identify genomic mutations associated with antibiotic resistance simultaneously can enable antibiotic stewardship and mitigate the spread of antibiotic-resistant MG. Toward this end, we first developed a multiplexed probe-based PCR-melt assay that detects MG and the presence of macrolide resistance mutations in the 23S rRNA gene and fluoroquinolone resistance mutations in the parC gene. Each target was identified via its unique combination of fluorescence label and melting temperature. This approach allowed differentiation between the different types of mutations at the genes of interest. Following initial assay optimization, the assay was integrated into a droplet magnetofluidic cartridge used in a portable platform to integrate automated sample extraction, PCR amplification, and detection. Lastly, we demonstrated that the integrated assay and droplet magnetofluidic platform could detect MG and antibiotic resistance-associated mutations in clinical isolates spiked into urine samples in 40 min.},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7258','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_abstract\" id=\"tp_abstract_7258\" style=\"display:none;\"><div class=\"tp_abstract_entry\">Mycoplasma genitalium (MG) is an emerging sexually transmitted bacterium. Due to its fastidious and slow-growing nature, MG is difficult to detect through culture-based diagnostics. Like Neisseria gonorrheae, another bacterial pathogen linked to sexually transmitted infections (STIs), MG has developed resistance to macrolide and fluoroquinolone antibiotics used to treat STIs. The ability to detect MG and identify genomic mutations associated with antibiotic resistance simultaneously can enable antibiotic stewardship and mitigate the spread of antibiotic-resistant MG. Toward this end, we first developed a multiplexed probe-based PCR-melt assay that detects MG and the presence of macrolide resistance mutations in the 23S rRNA gene and fluoroquinolone resistance mutations in the parC gene. Each target was identified via its unique combination of fluorescence label and melting temperature. This approach allowed differentiation between the different types of mutations at the genes of interest. Following initial assay optimization, the assay was integrated into a droplet magnetofluidic cartridge used in a portable platform to integrate automated sample extraction, PCR amplification, and detection. Lastly, we demonstrated that the integrated assay and droplet magnetofluidic platform could detect MG and antibiotic resistance-associated mutations in clinical isolates spiked into urine samples in 40 min.<\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7258','tp_abstract')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7258\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/36961769\/\" title=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/36961769\/\" target=\"_blank\">https:\/\/pubmed.ncbi.nlm.nih.gov\/36961769\/<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/DOI: 10.1021\/acssensors.2c02630\" title=\"Follow DOI:DOI: 10.1021\/acssensors.2c02630\" target=\"_blank\">doi:DOI: 10.1021\/acssensors.2c02630<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7258','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">Y. Zhao; CM. O&#039;Keefe; K. Hsieh; L. Cope; SC. Joyce; TR. Pisanic; JG. Herman; TH. Wang <\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7256','tp_links')\" style=\"cursor:pointer;\">Multiplex Digital Methylation\u2010Specific PCR for Noninvasive Screening of Lung Cancer<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Advanced Science, <\/span><span class=\"tp_pub_additional_volume\">vol. e2206518, <\/span><span class=\"tp_pub_additional_year\">2023<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_abstract_link\"><a id=\"tp_abstract_sh_7256\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7256','tp_abstract')\" title=\"Show abstract\" style=\"cursor:pointer;\">Abstract<\/a><\/span> | <span class=\"tp_resource_link\"><a id=\"tp_links_sh_7256\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7256','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7256\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7256','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7256\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Zhao2023,<br \/>\r\ntitle = {Multiplex Digital Methylation\u2010Specific PCR for Noninvasive Screening of Lung Cancer},<br \/>\r\nauthor = {Y. Zhao and CM. O&#039;Keefe and K. Hsieh and L. Cope and SC. Joyce and TR. Pisanic and JG. Herman and TH. Wang },<br \/>\r\nurl = {https:\/\/pubmed.ncbi.nlm.nih.gov\/37039321\/},<br \/>\r\nyear  = {2023},<br \/>\r\ndate = {2023-04-11},<br \/>\r\njournal = {Advanced Science},<br \/>\r\nvolume = {e2206518},<br \/>\r\nabstract = {There remains tremendous interest in developing liquid biopsy assays for detection of cancer-specific alterations, such as mutations and DNA methylation, in cell-free DNA (cfDNA) obtained through noninvasive blood draws. However, liquid biopsy analysis is often challenging due to exceedingly low fractions of circulating tumor DNA (ctDNA), necessitating the use of extended tumor biomarker panels. While multiplexed PCR strategies provide advantages such as higher throughput, their implementation is often hindered by challenges such as primer-dimers and PCR competition. Alternatively, digital PCR (dPCR) approaches generally offer superior performance, but with constrained multiplexing capability. This paper describes development and validation of the first multiplex digital methylation-specific PCR (mdMSP) platform for simultaneous analysis of four methylation biomarkers for liquid-biopsy-based detection of non-small cell lung cancer (NSCLC). mdMSP employs a microfluidic device containing four independent, but identical modules, housing a total of 40 160 nanowells. Analytical validation of the mdMSP platform demonstrates multiplex detection at analytical specificities as low as 0.0005%. The clinical utility of mdMSP is also demonstrated in a cohort of 72 clinical samples of low-volume liquid biopsy specimens from patients with computed tomography (CT)-scan indeterminant pulmonary nodules, exhibiting superior clinical performance when compared to traditional MSP assays for noninvasive detection of early-stage NSCLC.},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7256','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_abstract\" id=\"tp_abstract_7256\" style=\"display:none;\"><div class=\"tp_abstract_entry\">There remains tremendous interest in developing liquid biopsy assays for detection of cancer-specific alterations, such as mutations and DNA methylation, in cell-free DNA (cfDNA) obtained through noninvasive blood draws. However, liquid biopsy analysis is often challenging due to exceedingly low fractions of circulating tumor DNA (ctDNA), necessitating the use of extended tumor biomarker panels. While multiplexed PCR strategies provide advantages such as higher throughput, their implementation is often hindered by challenges such as primer-dimers and PCR competition. Alternatively, digital PCR (dPCR) approaches generally offer superior performance, but with constrained multiplexing capability. This paper describes development and validation of the first multiplex digital methylation-specific PCR (mdMSP) platform for simultaneous analysis of four methylation biomarkers for liquid-biopsy-based detection of non-small cell lung cancer (NSCLC). mdMSP employs a microfluidic device containing four independent, but identical modules, housing a total of 40 160 nanowells. Analytical validation of the mdMSP platform demonstrates multiplex detection at analytical specificities as low as 0.0005%. The clinical utility of mdMSP is also demonstrated in a cohort of 72 clinical samples of low-volume liquid biopsy specimens from patients with computed tomography (CT)-scan indeterminant pulmonary nodules, exhibiting superior clinical performance when compared to traditional MSP assays for noninvasive detection of early-stage NSCLC.<\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7256','tp_abstract')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7256\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/37039321\/\" title=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/37039321\/\" target=\"_blank\">https:\/\/pubmed.ncbi.nlm.nih.gov\/37039321\/<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7256','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">F.E. Chen, J. Wang, A.H. Nambiar, J. Hardick, J. Melendez, A.Y. Trick, T.H. Wang<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7315','tp_links')\" style=\"cursor:pointer;\">Point-of-Care Amenable Detection of Mycoplasma genitalium and Its Antibiotic Resistance Mutations<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">ACS Sensors, <\/span><span class=\"tp_pub_additional_volume\">vol. 8, <\/span><span class=\"tp_pub_additional_issue\">iss. 4, <\/span><span class=\"tp_pub_additional_pages\">pp. 1550-1557, <\/span><span class=\"tp_pub_additional_year\">2023<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7315\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7315','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7315\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7315','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7315\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Chen2023b,<br \/>\r\ntitle = {Point-of-Care Amenable Detection of Mycoplasma genitalium and Its Antibiotic Resistance Mutations},<br \/>\r\nauthor = {F.E. Chen, J. Wang, A.H. Nambiar, J. Hardick, J. Melendez, A.Y. Trick, T.H. Wang},<br \/>\r\nurl = {https:\/\/pubs.acs.org\/doi\/10.1021\/acssensors.2c02630},<br \/>\r\ndoi = {doi\/10.1021\/acssensors.2c02630},<br \/>\r\nyear  = {2023},<br \/>\r\ndate = {2023-03-24},<br \/>\r\njournal = {ACS Sensors},<br \/>\r\nvolume = {8},<br \/>\r\nissue = {4},<br \/>\r\npages = {1550-1557},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7315','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7315\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/pubs.acs.org\/doi\/10.1021\/acssensors.2c02630\" title=\"https:\/\/pubs.acs.org\/doi\/10.1021\/acssensors.2c02630\" target=\"_blank\">https:\/\/pubs.acs.org\/doi\/10.1021\/acssensors.2c02630<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/doi\/10.1021\/acssensors.2c02630\" title=\"Follow DOI:doi\/10.1021\/acssensors.2c02630\" target=\"_blank\">doi:doi\/10.1021\/acssensors.2c02630<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7315','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">X. Zhu, S. Sakamoto, C. Ishii, M.D. Smith, Koki Ito, M. Obayashi, L. Unger, Y. Hasegawa, S. Kurokawa, T. Kishimoto, H. L, S. Hatano, T.H. Wang, Y. Yoshikai, S. Kano, S. Fukuda, K. Sanada1, P.A. Calabresi, A. Kamiya<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7317','tp_links')\" style=\"cursor:pointer;\">Dectin-1 signaling on colonic \u03b3\u03b4 T cells promotes psychosocial stress responses<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Nature Immunology, <\/span><span class=\"tp_pub_additional_volume\">vol. 24, <\/span><span class=\"tp_pub_additional_number\">no. 625\u2013636, <\/span><span class=\"tp_pub_additional_year\">2023<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7317\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7317','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7317\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7317','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7317\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Zhu2023b,<br \/>\r\ntitle = {Dectin-1 signaling on colonic \u03b3\u03b4 T cells promotes psychosocial stress responses},<br \/>\r\nauthor = {X. Zhu, S. Sakamoto, C. Ishii, M.D. Smith, Koki Ito, M. Obayashi, L. Unger, Y. Hasegawa, S. Kurokawa, T. Kishimoto, H. L, S. Hatano, T.H. Wang, Y. Yoshikai, S. Kano, S. Fukuda, K. Sanada1, P.A. Calabresi, A. Kamiya},<br \/>\r\nurl = {https:\/\/www.nature.com\/articles\/s41590-023-01447-8},<br \/>\r\ndoi = {https:\/\/doi.org\/10.1038\/s41590-023-01447-8},<br \/>\r\nyear  = {2023},<br \/>\r\ndate = {2023-03-20},<br \/>\r\njournal = {Nature Immunology},<br \/>\r\nvolume = {24},<br \/>\r\nnumber = {625\u2013636},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7317','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7317\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/www.nature.com\/articles\/s41590-023-01447-8\" title=\"https:\/\/www.nature.com\/articles\/s41590-023-01447-8\" target=\"_blank\">https:\/\/www.nature.com\/articles\/s41590-023-01447-8<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/https:\/\/doi.org\/10.1038\/s41590-023-01447-8\" title=\"Follow DOI:https:\/\/doi.org\/10.1038\/s41590-023-01447-8\" target=\"_blank\">doi:https:\/\/doi.org\/10.1038\/s41590-023-01447-8<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7317','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">X. Zhu; S. Sakamoto; C. Ishii; MD. Smith; K. Ito; M. Obayashi; L. Unger; Y. Hasegawa; S. Kurokawa; T. Kishimoto; H. Li; S. Hatano; TH. Wang; Y. Yoshikai; S. Kano; S. Fukuda; K. Sanada; PA. Calabresi; A. Kamiya\r\n<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7260','tp_links')\" style=\"cursor:pointer;\">Dectin-1 signaling on colonic \u03b3\u03b4 T cells promotes psychosocial stress responses<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Nature Immunology, <\/span><span class=\"tp_pub_additional_volume\">vol. 24, <\/span><span class=\"tp_pub_additional_pages\">pp. 625-636, <\/span><span class=\"tp_pub_additional_year\">2023<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_abstract_link\"><a id=\"tp_abstract_sh_7260\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7260','tp_abstract')\" title=\"Show abstract\" style=\"cursor:pointer;\">Abstract<\/a><\/span> | <span class=\"tp_resource_link\"><a id=\"tp_links_sh_7260\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7260','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7260\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7260','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7260\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Zhu2023,<br \/>\r\ntitle = {Dectin-1 signaling on colonic \u03b3\u03b4 T cells promotes psychosocial stress responses},<br \/>\r\nauthor = {X. Zhu and S. Sakamoto and C. Ishii and MD. Smith and K. Ito and M. Obayashi and L. Unger and Y. Hasegawa and S. Kurokawa and T. Kishimoto and H. Li and S. Hatano and TH. Wang and Y. Yoshikai and S. Kano and S. Fukuda and K. Sanada and PA. Calabresi and A. Kamiya<br \/>\r\n},<br \/>\r\nurl = {https:\/\/pubmed.ncbi.nlm.nih.gov\/36941398\/},<br \/>\r\ndoi = {DOI: 10.1038\/s41590-023-01447-8},<br \/>\r\nyear  = {2023},<br \/>\r\ndate = {2023-03-20},<br \/>\r\njournal = {Nature Immunology},<br \/>\r\nvolume = {24},<br \/>\r\npages = {625-636},<br \/>\r\nabstract = {The intestinal immune system interacts with commensal microbiota to maintain gut homeostasis. Furthermore, stress alters the microbiome composition, leading to impaired brain function; yet how the intestinal immune system mediates these effects remains elusive. Here we report that colonic \u03b3\u03b4 T cells modulate behavioral vulnerability to chronic social stress via dectin-1 signaling. We show that reduction in specific Lactobacillus species, which are involved in T cell differentiation to protect the host immune system, contributes to stress-induced social-avoidance behavior, consistent with our observations in patients with depression. Stress-susceptible behaviors derive from increased differentiation in colonic interleukin (IL)-17-producing \u03b3\u03b4 T cells (\u03b3\u03b417 T cells) and their meningeal accumulation. These stress-susceptible cellular and behavioral phenotypes are causally mediated by dectin-1, an innate immune receptor expressed in \u03b3\u03b4 T cells. Our results highlight the previously unrecognized role of intestinal \u03b3\u03b417 T cells in the modulation of psychological stress responses and the importance of dectin-1 as a potential therapeutic target for the treatment of stress-induced behaviors.},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7260','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_abstract\" id=\"tp_abstract_7260\" style=\"display:none;\"><div class=\"tp_abstract_entry\">The intestinal immune system interacts with commensal microbiota to maintain gut homeostasis. Furthermore, stress alters the microbiome composition, leading to impaired brain function; yet how the intestinal immune system mediates these effects remains elusive. Here we report that colonic \u03b3\u03b4 T cells modulate behavioral vulnerability to chronic social stress via dectin-1 signaling. We show that reduction in specific Lactobacillus species, which are involved in T cell differentiation to protect the host immune system, contributes to stress-induced social-avoidance behavior, consistent with our observations in patients with depression. Stress-susceptible behaviors derive from increased differentiation in colonic interleukin (IL)-17-producing \u03b3\u03b4 T cells (\u03b3\u03b417 T cells) and their meningeal accumulation. These stress-susceptible cellular and behavioral phenotypes are causally mediated by dectin-1, an innate immune receptor expressed in \u03b3\u03b4 T cells. Our results highlight the previously unrecognized role of intestinal \u03b3\u03b417 T cells in the modulation of psychological stress responses and the importance of dectin-1 as a potential therapeutic target for the treatment of stress-induced behaviors.<\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7260','tp_abstract')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7260\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/36941398\/\" title=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/36941398\/\" target=\"_blank\">https:\/\/pubmed.ncbi.nlm.nih.gov\/36941398\/<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/DOI: 10.1038\/s41590-023-01447-8\" title=\"Follow DOI:DOI: 10.1038\/s41590-023-01447-8\" target=\"_blank\">doi:DOI: 10.1038\/s41590-023-01447-8<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7260','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">F. Shao; JS. Park; G. Zhao,; K. Hsieh; TH. Wang<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7264','tp_links')\" style=\"cursor:pointer;\">Elucidating the Role of CRISPR\/Cas in Single-Step Isothermal Nucle-ic Acid Amplification Testing Assays<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Analytical Chemistry, <\/span><span class=\"tp_pub_additional_volume\">vol. 95, <\/span><span class=\"tp_pub_additional_issue\">iss. 7, <\/span><span class=\"tp_pub_additional_pages\">pp. 3873-3882, <\/span><span class=\"tp_pub_additional_year\">2023<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_abstract_link\"><a id=\"tp_abstract_sh_7264\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7264','tp_abstract')\" title=\"Show abstract\" style=\"cursor:pointer;\">Abstract<\/a><\/span> | <span class=\"tp_resource_link\"><a id=\"tp_links_sh_7264\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7264','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7264\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7264','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7264\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Shao2023,<br \/>\r\ntitle = {Elucidating the Role of CRISPR\/Cas in Single-Step Isothermal Nucle-ic Acid Amplification Testing Assays},<br \/>\r\nauthor = {F. Shao and JS. Park and G. Zhao, and K. Hsieh and TH. Wang},<br \/>\r\nurl = {https:\/\/pubmed.ncbi.nlm.nih.gov\/36745596\/},<br \/>\r\ndoi = {DOI: 10.1021\/acs.analchem.2c05632},<br \/>\r\nyear  = {2023},<br \/>\r\ndate = {2023-02-21},<br \/>\r\njournal = {Analytical Chemistry},<br \/>\r\nvolume = {95},<br \/>\r\nissue = {7},<br \/>\r\npages = {3873-3882},<br \/>\r\nabstract = {Developing assays that combine CRISPR\/Cas and isothermal nucleic acid amplification has become a burgeoning research area due to the novelty and simplicity of CRISPR\/Cas and the potential for point-of-care uses. Most current research explores various two-step assays by appending different CRISPR\/Cas effectors to the end of different isothermal nucleic acid amplification methods. However, efforts in integrating both components into more ideal single-step assays are scarce, and poor-performing single-step assays have been reported. Moreover, lack of investigations into CRISPR\/Cas in single-step assays results in incomplete understanding. To fill this knowledge gap, we conducted a systematic investigation by developing and comparing assays that share the identical recombinase polymerase amplification (RPA) but differ in CRISPR\/Cas12a. We found that the addition of CRISPR\/Cas12a indeed unlocks signal amplification but, at the same time, impedes RPA and that CRISPR\/Cas12a concentration is a key parameter for attenuating RPA impediment and ensuring assay performance. Accordingly, we found that our protospacer adjacent motif (PAM)-free CRISPR\/Cas12a-assisted RPA assay, which only moderately impeded RPA at its optimal CRISPR\/Cas12a concentration, outperformed its counterparts in assay design, signal, sensitivity, and speed. We also discovered that a new commercial Cas12a effector could also drive our PAM-free CRISPR\/Cas12a-assisted RPA assay and reduce its cost, though simultaneously lowering its signal. Our study and the new insights can be broadly applied to steer and facilitate further advances in CRISPR\/Cas-based assays.},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7264','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_abstract\" id=\"tp_abstract_7264\" style=\"display:none;\"><div class=\"tp_abstract_entry\">Developing assays that combine CRISPR\/Cas and isothermal nucleic acid amplification has become a burgeoning research area due to the novelty and simplicity of CRISPR\/Cas and the potential for point-of-care uses. Most current research explores various two-step assays by appending different CRISPR\/Cas effectors to the end of different isothermal nucleic acid amplification methods. However, efforts in integrating both components into more ideal single-step assays are scarce, and poor-performing single-step assays have been reported. Moreover, lack of investigations into CRISPR\/Cas in single-step assays results in incomplete understanding. To fill this knowledge gap, we conducted a systematic investigation by developing and comparing assays that share the identical recombinase polymerase amplification (RPA) but differ in CRISPR\/Cas12a. We found that the addition of CRISPR\/Cas12a indeed unlocks signal amplification but, at the same time, impedes RPA and that CRISPR\/Cas12a concentration is a key parameter for attenuating RPA impediment and ensuring assay performance. Accordingly, we found that our protospacer adjacent motif (PAM)-free CRISPR\/Cas12a-assisted RPA assay, which only moderately impeded RPA at its optimal CRISPR\/Cas12a concentration, outperformed its counterparts in assay design, signal, sensitivity, and speed. We also discovered that a new commercial Cas12a effector could also drive our PAM-free CRISPR\/Cas12a-assisted RPA assay and reduce its cost, though simultaneously lowering its signal. Our study and the new insights can be broadly applied to steer and facilitate further advances in CRISPR\/Cas-based assays.<\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7264','tp_abstract')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7264\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/36745596\/\" title=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/36745596\/\" target=\"_blank\">https:\/\/pubmed.ncbi.nlm.nih.gov\/36745596\/<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/DOI: 10.1021\/acs.analchem.2c05632\" title=\"Follow DOI:DOI: 10.1021\/acs.analchem.2c05632\" target=\"_blank\">doi:DOI: 10.1021\/acs.analchem.2c05632<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7264','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">F. Shao, J. S. Park, G. Zhao, K. Hsieh, T.H. Wang<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7321','tp_links')\" style=\"cursor:pointer;\">Elucidating the Role of CRISPR\/Cas in Single-Step Isothermal Nucleic Acid Amplification Testing Assays<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Analytical Chemistry, <\/span><span class=\"tp_pub_additional_volume\">vol. 95, <\/span><span class=\"tp_pub_additional_issue\">iss. 7, <\/span><span class=\"tp_pub_additional_pages\">pp. 3873-3882, <\/span><span class=\"tp_pub_additional_year\">2023<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7321\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7321','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7321\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7321','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7321\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Shao2023b,<br \/>\r\ntitle = {Elucidating the Role of CRISPR\/Cas in Single-Step Isothermal Nucleic Acid Amplification Testing Assays},<br \/>\r\nauthor = {F. Shao, J. S. Park, G. Zhao, K. Hsieh, T.H. Wang},<br \/>\r\nurl = {https:\/\/pubs.acs.org\/doi\/10.1021\/acs.analchem.2c05632},<br \/>\r\ndoi = {doi\/10.1021\/acs.analchem.2c05632.},<br \/>\r\nyear  = {2023},<br \/>\r\ndate = {2023-02-06},<br \/>\r\njournal = {Analytical Chemistry},<br \/>\r\nvolume = {95},<br \/>\r\nissue = {7},<br \/>\r\npages = {3873-3882},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7321','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7321\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/pubs.acs.org\/doi\/10.1021\/acs.analchem.2c05632\" title=\"https:\/\/pubs.acs.org\/doi\/10.1021\/acs.analchem.2c05632\" target=\"_blank\">https:\/\/pubs.acs.org\/doi\/10.1021\/acs.analchem.2c05632<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/doi\/10.1021\/acs.analchem.2c05632.\" title=\"Follow DOI:doi\/10.1021\/acs.analchem.2c05632.\" target=\"_blank\">doi:doi\/10.1021\/acs.analchem.2c05632.<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7321','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">H. Li; K. Hsieh; P. Wong; K. Mach; J. Liao; TH. Wang<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7266','tp_links')\" style=\"cursor:pointer;\">Single-cell pathogen diagnostics for combating antibiotic resistance<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Nature Reviews Methods Primers, <\/span><span class=\"tp_pub_additional_volume\">vol. 3, <\/span><span class=\"tp_pub_additional_issue\">iss. 1, <\/span><span class=\"tp_pub_additional_number\">no. 6, <\/span><span class=\"tp_pub_additional_year\">2023<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7266\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7266','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7266\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7266','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7266\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Li2023,<br \/>\r\ntitle = {Single-cell pathogen diagnostics for combating antibiotic resistance},<br \/>\r\nauthor = {H. Li and K. Hsieh and P. Wong and K. Mach and J. Liao and TH. Wang},<br \/>\r\nurl = {https:\/\/www.nature.com\/articles\/s43586-023-00201-6},<br \/>\r\ndoi = {DOI: 10.1021\/acs.accounts.1c00462},<br \/>\r\nyear  = {2023},<br \/>\r\ndate = {2023-02-02},<br \/>\r\njournal = {Nature Reviews Methods Primers},<br \/>\r\nvolume = {3},<br \/>\r\nnumber = {6},<br \/>\r\nissue = {1},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7266','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7266\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/www.nature.com\/articles\/s43586-023-00201-6\" title=\"https:\/\/www.nature.com\/articles\/s43586-023-00201-6\" target=\"_blank\">https:\/\/www.nature.com\/articles\/s43586-023-00201-6<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/DOI: 10.1021\/acs.accounts.1c00462\" title=\"Follow DOI:DOI: 10.1021\/acs.accounts.1c00462\" target=\"_blank\">doi:DOI: 10.1021\/acs.accounts.1c00462<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7266','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">H. Li, K. Hsieh, P. Wong, K. Mach, J. Liao, T.H, Wang<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7323','tp_links')\" style=\"cursor:pointer;\">Single-cell pathogen diagnostics for combating antibiotic resistance<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Nature Reviews Methods Primers, <\/span><span class=\"tp_pub_additional_volume\">vol. 95, <\/span><span class=\"tp_pub_additional_issue\">iss. 7, <\/span><span class=\"tp_pub_additional_year\">2023<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7323\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7323','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7323\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7323','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7323\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Li2023b,<br \/>\r\ntitle = {Single-cell pathogen diagnostics for combating antibiotic resistance},<br \/>\r\nauthor = {H. Li, K. Hsieh, P. Wong, K. Mach, J. Liao, T.H, Wang},<br \/>\r\nurl = {https:\/\/www.nature.com\/articles\/s43586-022-00190-y},<br \/>\r\ndoi = {DOI: 10.1038\/s43586-022-00190-y},<br \/>\r\nyear  = {2023},<br \/>\r\ndate = {2023-02-02},<br \/>\r\njournal = {Nature Reviews Methods Primers},<br \/>\r\nvolume = {95},<br \/>\r\nissue = {7},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7323','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7323\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/www.nature.com\/articles\/s43586-022-00190-y\" title=\"https:\/\/www.nature.com\/articles\/s43586-022-00190-y\" target=\"_blank\">https:\/\/www.nature.com\/articles\/s43586-022-00190-y<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/DOI: 10.1038\/s43586-022-00190-y\" title=\"Follow DOI:DOI: 10.1038\/s43586-022-00190-y\" target=\"_blank\">doi:DOI: 10.1038\/s43586-022-00190-y<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7323','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">HT. Ngo; M. Jin; AY. Trick; FE. Chen; L. Chen; K. Hsieh; TH. Wang\r\n<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7268','tp_links')\" style=\"cursor:pointer;\">Sensitive and Quantitative Point-of-Care HIV Viral Load Quantification from Blood Using a Power-Free Plasma Separation and Portable Magnetofluidic Polymerase Chain Reaction Instrument<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\"> Analytical Chemistry, <\/span><span class=\"tp_pub_additional_volume\">vol. 95, <\/span><span class=\"tp_pub_additional_issue\">iss. 2, <\/span><span class=\"tp_pub_additional_pages\">pp. 1159\u20131168, <\/span><span class=\"tp_pub_additional_year\">2023<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_abstract_link\"><a id=\"tp_abstract_sh_7268\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7268','tp_abstract')\" title=\"Show abstract\" style=\"cursor:pointer;\">Abstract<\/a><\/span> | <span class=\"tp_resource_link\"><a id=\"tp_links_sh_7268\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7268','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7268\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7268','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7268\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Ngo2023b,<br \/>\r\ntitle = {Sensitive and Quantitative Point-of-Care HIV Viral Load Quantification from Blood Using a Power-Free Plasma Separation and Portable Magnetofluidic Polymerase Chain Reaction Instrument},<br \/>\r\nauthor = {HT. Ngo and M. Jin and AY. Trick and FE. Chen and L. Chen and K. Hsieh and TH. Wang<br \/>\r\n},<br \/>\r\nurl = {https:\/\/pubmed.ncbi.nlm.nih.gov\/36562405\/},<br \/>\r\ndoi = {DOI: 10.1021\/acs.analchem.2c03897},<br \/>\r\nyear  = {2023},<br \/>\r\ndate = {2023-01-17},<br \/>\r\njournal = { Analytical Chemistry},<br \/>\r\nvolume = {95},<br \/>\r\nissue = {2},<br \/>\r\npages = {1159\u20131168},<br \/>\r\nabstract = {Point-of-care (POC) HIV viral load (VL) tests are needed to enhance access to HIV VL testing in low- and middle-income countries (LMICs) and to enable HIV VL self-testing at home, which in turn have the potential to enhance the global management of the disease. While methods based on real-time reverse transcription-polymerase chain reaction (RT-PCR) are highly sensitive and quantitatively accurate, they often require bulky and expensive instruments, making applications at the POC challenging. On the other hand, although methods based on isothermal amplification techniques could be performed using low-cost instruments, they have shown limited quantitative accuracies, i.e., being only semiquantitative. Herein, we present a sensitive and quantitative POC HIV VL quantification method from blood that can be performed using a small power-free three-dimensional-printed plasma separation device and a portable, low-cost magnetofluidic real-time RT-PCR instrument. The plasma separation device, which is composed of a plasma separation membrane and an absorbent material, demonstrated 96% plasma separation efficiency per 100 \u03bcL of whole blood. The plasma solution was then processed in a magnetofluidic cartridge for automated HIV RNA extraction and quantification using the portable instrument, which completed 50 cycles of PCR in 15 min. Using the method, we achieved a limit of detection of 500 HIV RNA copies\/mL, which is below the World Health Organization&#039;s virological failure threshold, and a good quantitative accuracy. The method has the potential for sensitive and quantitative HIV VL testing at the POC and at home self-testing.},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7268','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_abstract\" id=\"tp_abstract_7268\" style=\"display:none;\"><div class=\"tp_abstract_entry\">Point-of-care (POC) HIV viral load (VL) tests are needed to enhance access to HIV VL testing in low- and middle-income countries (LMICs) and to enable HIV VL self-testing at home, which in turn have the potential to enhance the global management of the disease. While methods based on real-time reverse transcription-polymerase chain reaction (RT-PCR) are highly sensitive and quantitatively accurate, they often require bulky and expensive instruments, making applications at the POC challenging. On the other hand, although methods based on isothermal amplification techniques could be performed using low-cost instruments, they have shown limited quantitative accuracies, i.e., being only semiquantitative. Herein, we present a sensitive and quantitative POC HIV VL quantification method from blood that can be performed using a small power-free three-dimensional-printed plasma separation device and a portable, low-cost magnetofluidic real-time RT-PCR instrument. The plasma separation device, which is composed of a plasma separation membrane and an absorbent material, demonstrated 96% plasma separation efficiency per 100 \u03bcL of whole blood. The plasma solution was then processed in a magnetofluidic cartridge for automated HIV RNA extraction and quantification using the portable instrument, which completed 50 cycles of PCR in 15 min. Using the method, we achieved a limit of detection of 500 HIV RNA copies\/mL, which is below the World Health Organization&#039;s virological failure threshold, and a good quantitative accuracy. The method has the potential for sensitive and quantitative HIV VL testing at the POC and at home self-testing.<\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7268','tp_abstract')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7268\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/36562405\/\" title=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/36562405\/\" target=\"_blank\">https:\/\/pubmed.ncbi.nlm.nih.gov\/36562405\/<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/DOI: 10.1021\/acs.analchem.2c03897\" title=\"Follow DOI:DOI: 10.1021\/acs.analchem.2c03897\" target=\"_blank\">doi:DOI: 10.1021\/acs.analchem.2c03897<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7268','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_bachelorthesis\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">F. Shao, P.W Lee, H. Li, K. Hsieh, T.H Wang <\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7327','tp_links')\" style=\"cursor:pointer;\">Emerging platforms for high-throughput enzymatic bioassays<\/a> <span class=\"tp_pub_type tp_  bachelorthesis\">Bachelor Thesis<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_year\">2023<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7327\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7327','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7327\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7327','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7327\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@bachelorthesis{Shao2023c,<br \/>\r\ntitle = {Emerging platforms for high-throughput enzymatic bioassays},<br \/>\r\nauthor = {F. Shao, P.W Lee, H. Li, K. Hsieh, T.H Wang },<br \/>\r\nurl = {https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0167779922001469},<br \/>\r\ndoi = {https:\/\/doi.org\/10.1016\/j.tibtech.2022.06.006},<br \/>\r\nyear  = {2023},<br \/>\r\ndate = {2023-01-10},<br \/>\r\njournal = {Trends in Biotechnology},<br \/>\r\nvolume = {41},<br \/>\r\nissue = {1},<br \/>\r\npages = {120-133},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {bachelorthesis}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7327','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7327\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0167779922001469\" title=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0167779922001469\" target=\"_blank\">https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0167779922001469<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/https:\/\/doi.org\/10.1016\/j.tibtech.2022.06.006\" title=\"Follow DOI:https:\/\/doi.org\/10.1016\/j.tibtech.2022.06.006\" target=\"_blank\">doi:https:\/\/doi.org\/10.1016\/j.tibtech.2022.06.006<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7327','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><h3 class=\"tp_h3\" id=\"tp_h3_2022\">2022<\/h3><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">H. T. Ngo, M. Jin, A.Y. Trick, F.E. Chen, L. Chen, K. Hsieh, T.H. Wang<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7325','tp_links')\" style=\"cursor:pointer;\">Sensitive and Quantitative Point-of-Care HIV Viral Load Quantification from Blood Using a Power-Free Plasma Separation and Portable Magnetofluidic Polymerase Chain Reaction Instrument<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Analytical Chemistry, <\/span><span class=\"tp_pub_additional_volume\">vol. 9, <\/span><span class=\"tp_pub_additional_issue\">iss. 2, <\/span><span class=\"tp_pub_additional_year\">2022<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_resource_link\"><a id=\"tp_links_sh_7325\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7325','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7325\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7325','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7325\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Ngo2022,<br \/>\r\ntitle = {Sensitive and Quantitative Point-of-Care HIV Viral Load Quantification from Blood Using a Power-Free Plasma Separation and Portable Magnetofluidic Polymerase Chain Reaction Instrument},<br \/>\r\nauthor = {H. T. Ngo, M. Jin, A.Y. Trick, F.E. Chen, L. Chen, K. Hsieh, T.H. Wang},<br \/>\r\nurl = {https:\/\/pubs.acs.org\/doi\/full\/10.1021\/acs.analchem.2c03897},<br \/>\r\ndoi = {doi\/10.1021\/acs.analchem.2c03897.},<br \/>\r\nyear  = {2022},<br \/>\r\ndate = {2022-12-23},<br \/>\r\njournal = {Analytical Chemistry},<br \/>\r\nvolume = {9},<br \/>\r\nissue = {2},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7325','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7325\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/pubs.acs.org\/doi\/full\/10.1021\/acs.analchem.2c03897\" title=\"https:\/\/pubs.acs.org\/doi\/full\/10.1021\/acs.analchem.2c03897\" target=\"_blank\">https:\/\/pubs.acs.org\/doi\/full\/10.1021\/acs.analchem.2c03897<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/doi\/10.1021\/acs.analchem.2c03897.\" title=\"Follow DOI:doi\/10.1021\/acs.analchem.2c03897.\" target=\"_blank\">doi:doi\/10.1021\/acs.analchem.2c03897.<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7325','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">P. Akarapipad; E. Bertelson; A. Pessell; TH. Wang; K. Hsieh\r\n<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7274','tp_links')\" style=\"cursor:pointer;\">Emerging Multiplex Nucleic Acid Diagnostic Tests for Combating COVID-19<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Biosensors, <\/span><span class=\"tp_pub_additional_volume\">vol. 12, <\/span><span class=\"tp_pub_additional_issue\">iss. 11, <\/span><span class=\"tp_pub_additional_pages\">pp. 978, <\/span><span class=\"tp_pub_additional_year\">2022<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_abstract_link\"><a id=\"tp_abstract_sh_7274\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7274','tp_abstract')\" title=\"Show abstract\" style=\"cursor:pointer;\">Abstract<\/a><\/span> | <span class=\"tp_resource_link\"><a id=\"tp_links_sh_7274\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7274','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7274\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7274','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7274\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Akarapipad2022,<br \/>\r\ntitle = {Emerging Multiplex Nucleic Acid Diagnostic Tests for Combating COVID-19},<br \/>\r\nauthor = {P. Akarapipad and E. Bertelson and A. Pessell and TH. Wang and K. Hsieh<br \/>\r\n},<br \/>\r\nurl = {https:\/\/pubmed.ncbi.nlm.nih.gov\/36354487\/},<br \/>\r\ndoi = {DOI: 10.3390\/bios12110978},<br \/>\r\nyear  = {2022},<br \/>\r\ndate = {2022-11-07},<br \/>\r\nurldate = {2022-11-07},<br \/>\r\njournal = {Biosensors},<br \/>\r\nvolume = {12},<br \/>\r\nissue = {11},<br \/>\r\npages = {978},<br \/>\r\nabstract = {The COVID-19 pandemic caused by SARS-CoV-2 has drawn attention to the need for fast and accurate diagnostic testing. Concerns from emerging SARS-CoV-2 variants and other circulating respiratory viral pathogens further underscore the importance of expanding diagnostic testing to multiplex detection, as single-plex diagnostic testing may fail to detect emerging variants and other viruses, while sequencing can be too slow and too expensive as a diagnostic tool. As a result, there have been significant advances in multiplex nucleic-acid-based virus diagnostic testing, creating a need for a timely review. This review first introduces frequent nucleic acid targets for multiplex virus diagnostic tests, then proceeds to a comprehensive and up-to-date overview of multiplex assays that incorporate various detection reactions and readout modalities. The performances, advantages, and disadvantages of these assays are discussed, followed by highlights of platforms that are amenable for point-of-care use. Finally, this review points out the remaining technical challenges and shares perspectives on future research and development. By examining the state of the art and synthesizing existing development in multiplex nucleic acid diagnostic tests, this review can provide a useful resource for facilitating future research and ultimately combating COVID-19.},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7274','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_abstract\" id=\"tp_abstract_7274\" style=\"display:none;\"><div class=\"tp_abstract_entry\">The COVID-19 pandemic caused by SARS-CoV-2 has drawn attention to the need for fast and accurate diagnostic testing. Concerns from emerging SARS-CoV-2 variants and other circulating respiratory viral pathogens further underscore the importance of expanding diagnostic testing to multiplex detection, as single-plex diagnostic testing may fail to detect emerging variants and other viruses, while sequencing can be too slow and too expensive as a diagnostic tool. As a result, there have been significant advances in multiplex nucleic-acid-based virus diagnostic testing, creating a need for a timely review. This review first introduces frequent nucleic acid targets for multiplex virus diagnostic tests, then proceeds to a comprehensive and up-to-date overview of multiplex assays that incorporate various detection reactions and readout modalities. The performances, advantages, and disadvantages of these assays are discussed, followed by highlights of platforms that are amenable for point-of-care use. Finally, this review points out the remaining technical challenges and shares perspectives on future research and development. By examining the state of the art and synthesizing existing development in multiplex nucleic acid diagnostic tests, this review can provide a useful resource for facilitating future research and ultimately combating COVID-19.<\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7274','tp_abstract')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7274\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/36354487\/\" title=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/36354487\/\" target=\"_blank\">https:\/\/pubmed.ncbi.nlm.nih.gov\/36354487\/<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/DOI: 10.3390\/bios12110978\" title=\"Follow DOI:DOI: 10.3390\/bios12110978\" target=\"_blank\">doi:DOI: 10.3390\/bios12110978<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7274','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">S. Li; Y. Hu; A. Li; J. Lin; K. Hsieh; Z. Schneiderman; P. Zhang; Y. Zhu; C. Qiu; E. Kokkoli; TH. Wang; HQ. Mao<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7229','tp_links')\" style=\"cursor:pointer;\">Payload distribution and capacity of mRNA lipid nanoparticles<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">nature communications, <\/span><span class=\"tp_pub_additional_volume\">vol. 13, <\/span><span class=\"tp_pub_additional_number\">no. 5561, <\/span><span class=\"tp_pub_additional_year\">2022<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_abstract_link\"><a id=\"tp_abstract_sh_7229\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7229','tp_abstract')\" title=\"Show abstract\" style=\"cursor:pointer;\">Abstract<\/a><\/span> | <span class=\"tp_resource_link\"><a id=\"tp_links_sh_7229\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7229','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7229\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7229','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7229\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Li2022,<br \/>\r\ntitle = {Payload distribution and capacity of mRNA lipid nanoparticles},<br \/>\r\nauthor = {S. Li and Y. Hu and A. Li and J. Lin and K. Hsieh and Z. Schneiderman and P. Zhang and Y. Zhu and C. Qiu and E. Kokkoli and TH. Wang and HQ. Mao},<br \/>\r\ndoi = {https:\/\/doi.org\/10.1038\/s41467-022-33157-4},<br \/>\r\nyear  = {2022},<br \/>\r\ndate = {2022-09-23},<br \/>\r\njournal = {nature communications},<br \/>\r\nvolume = {13},<br \/>\r\nnumber = {5561},<br \/>\r\nabstract = {Lipid nanoparticles (LNPs) are effective vehicles to deliver mRNA vaccines and therapeutics. It has been challenging to assess mRNA packaging characteristics in LNPs, including payload distribution and capacity, which are critical to understanding structure-property-function relationships for further carrier development. Here, we report a method based on the multi-laser cylindrical illumination confocal spectroscopy (CICS) technique to examine mRNA and lipid contents in LNP formulations at the single-nanoparticle level. By differentiating unencapsulated mRNAs, empty LNPs and mRNA-loaded LNPs via coincidence analysis of fluorescent tags on different LNP components, and quantitatively resolving single-mRNA fluorescence, we reveal that a commonly referenced benchmark formulation using DLin-MC3 as the ionizable lipid contains mostly 2 mRNAs per loaded LNP with a presence of 40%\u201380% empty LNPs depending on the assembly conditions. Systematic analysis of different formulations with control variables reveals a kinetically controlled assembly mechanism that governs the payload distribution and capacity in LNPs. These results form the foundation for a holistic understanding of the molecular assembly of mRNA LNPs.},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7229','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_abstract\" id=\"tp_abstract_7229\" style=\"display:none;\"><div class=\"tp_abstract_entry\">Lipid nanoparticles (LNPs) are effective vehicles to deliver mRNA vaccines and therapeutics. It has been challenging to assess mRNA packaging characteristics in LNPs, including payload distribution and capacity, which are critical to understanding structure-property-function relationships for further carrier development. Here, we report a method based on the multi-laser cylindrical illumination confocal spectroscopy (CICS) technique to examine mRNA and lipid contents in LNP formulations at the single-nanoparticle level. By differentiating unencapsulated mRNAs, empty LNPs and mRNA-loaded LNPs via coincidence analysis of fluorescent tags on different LNP components, and quantitatively resolving single-mRNA fluorescence, we reveal that a commonly referenced benchmark formulation using DLin-MC3 as the ionizable lipid contains mostly 2 mRNAs per loaded LNP with a presence of 40%\u201380% empty LNPs depending on the assembly conditions. Systematic analysis of different formulations with control variables reveals a kinetically controlled assembly mechanism that governs the payload distribution and capacity in LNPs. These results form the foundation for a holistic understanding of the molecular assembly of mRNA LNPs.<\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7229','tp_abstract')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7229\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/https:\/\/doi.org\/10.1038\/s41467-022-33157-4\" title=\"Follow DOI:https:\/\/doi.org\/10.1038\/s41467-022-33157-4\" target=\"_blank\">doi:https:\/\/doi.org\/10.1038\/s41467-022-33157-4<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7229','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">P. Zhang; J. Hu; JS. Park; K. Hsieh; L. Chen; A. Mao; TH. Wang<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7233','tp_links')\" style=\"cursor:pointer;\">Highly Sensitive Serum Protein Analysis Using Magnetic Bead-Based Proximity Extension Assay<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Analytical Chemistry, <\/span><span class=\"tp_pub_additional_volume\">vol. 94, <\/span><span class=\"tp_pub_additional_issue\">iss. 36, <\/span><span class=\"tp_pub_additional_pages\">pp. 1281-12489, <\/span><span class=\"tp_pub_additional_year\">2022<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_abstract_link\"><a id=\"tp_abstract_sh_7233\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7233','tp_abstract')\" title=\"Show abstract\" style=\"cursor:pointer;\">Abstract<\/a><\/span> | <span class=\"tp_resource_link\"><a id=\"tp_links_sh_7233\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7233','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7233\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7233','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7233\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Zhang2022,<br \/>\r\ntitle = {Highly Sensitive Serum Protein Analysis Using Magnetic Bead-Based Proximity Extension Assay},<br \/>\r\nauthor = {P. Zhang and J. Hu and JS. Park and K. Hsieh and L. Chen and A. Mao and TH. Wang},<br \/>\r\nurl = {https:\/\/pubs.acs.org\/doi\/full\/10.1021\/acs.analchem.2c02684},<br \/>\r\ndoi = {https:\/\/doi.org\/10.1021\/acs.analchem.2c02684},<br \/>\r\nyear  = {2022},<br \/>\r\ndate = {2022-08-30},<br \/>\r\nurldate = {2022-08-30},<br \/>\r\njournal = {Analytical Chemistry},<br \/>\r\nvolume = {94},<br \/>\r\nissue = {36},<br \/>\r\npages = {1281-12489},<br \/>\r\nabstract = {Many protein biomarkers are present in biofluids at a very low level but may play critical roles in important biological processes. The fact that these low-abundance proteins remain largely unexplored underscores the importance of developing new tools for highly sensitive protein detection. Although digital enzyme-linked immunosorbent assay (ELISA) has demonstrated ultrahigh sensitivity compared with conventional ELISA, the requirement of specialized instruments limits the accessibility and prevents the widespread implementation. On the other hand, proximity ligation assays (PLA) and proximity extension assays (PEA) show sensitive and specific protein detection using regular laboratory setups, but their sensitivity needs to be further improved to match digital ELISA. To achieve highly sensitive protein detection with minimal accessibility limitation, we develop a magnetic bead-based PEA (magPEA), which posts triple epitope recognition requirement and enables extensive washing for improved sensitivity and enhanced specificity. We demonstrate that the incorporation of magnetic beads into PEA workflow facilitates orders of magnitude sensitivity improvement compared with conventional ELISA, homogeneous PEA, and solid-phase PLA and achieves limits of detection close to that of digital ELISA when using IL-6, IL-8, and GM-CSF as validation. Our magPEA provides a simple approach for highly sensitive protein detection that can be readily implemented to other laboratories and will thus ultimately accelerate the study of the low abundance protein biomarkers in the future.},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7233','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_abstract\" id=\"tp_abstract_7233\" style=\"display:none;\"><div class=\"tp_abstract_entry\">Many protein biomarkers are present in biofluids at a very low level but may play critical roles in important biological processes. The fact that these low-abundance proteins remain largely unexplored underscores the importance of developing new tools for highly sensitive protein detection. Although digital enzyme-linked immunosorbent assay (ELISA) has demonstrated ultrahigh sensitivity compared with conventional ELISA, the requirement of specialized instruments limits the accessibility and prevents the widespread implementation. On the other hand, proximity ligation assays (PLA) and proximity extension assays (PEA) show sensitive and specific protein detection using regular laboratory setups, but their sensitivity needs to be further improved to match digital ELISA. To achieve highly sensitive protein detection with minimal accessibility limitation, we develop a magnetic bead-based PEA (magPEA), which posts triple epitope recognition requirement and enables extensive washing for improved sensitivity and enhanced specificity. We demonstrate that the incorporation of magnetic beads into PEA workflow facilitates orders of magnitude sensitivity improvement compared with conventional ELISA, homogeneous PEA, and solid-phase PLA and achieves limits of detection close to that of digital ELISA when using IL-6, IL-8, and GM-CSF as validation. Our magPEA provides a simple approach for highly sensitive protein detection that can be readily implemented to other laboratories and will thus ultimately accelerate the study of the low abundance protein biomarkers in the future.<\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7233','tp_abstract')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7233\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/pubs.acs.org\/doi\/full\/10.1021\/acs.analchem.2c02684\" title=\"https:\/\/pubs.acs.org\/doi\/full\/10.1021\/acs.analchem.2c02684\" target=\"_blank\">https:\/\/pubs.acs.org\/doi\/full\/10.1021\/acs.analchem.2c02684<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/https:\/\/doi.org\/10.1021\/acs.analchem.2c02684\" title=\"Follow DOI:https:\/\/doi.org\/10.1021\/acs.analchem.2c02684\" target=\"_blank\">doi:https:\/\/doi.org\/10.1021\/acs.analchem.2c02684<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7233','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">F. Shao; K. Hsieh; P. Zhang; AM. Kaushik; TH. Wang<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7236','tp_links')\" style=\"cursor:pointer;\">Facile and scalable tubing-free sample loading for droplet microfluidics<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Scientific Reports, <\/span><span class=\"tp_pub_additional_volume\">vol. 12, <\/span><span class=\"tp_pub_additional_issue\">iss. 1, <\/span><span class=\"tp_pub_additional_number\">no. 13340, <\/span><span class=\"tp_pub_additional_pages\">pp. 1-12, <\/span><span class=\"tp_pub_additional_year\">2022<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_abstract_link\"><a id=\"tp_abstract_sh_7236\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7236','tp_abstract')\" title=\"Show abstract\" style=\"cursor:pointer;\">Abstract<\/a><\/span> | <span class=\"tp_resource_link\"><a id=\"tp_links_sh_7236\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7236','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7236\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7236','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7236\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{nokey,<br \/>\r\ntitle = {Facile and scalable tubing-free sample loading for droplet microfluidics},<br \/>\r\nauthor = {F. Shao and K. Hsieh and P. Zhang and AM. Kaushik and TH. Wang},<br \/>\r\nurl = {https:\/\/www.nature.com\/articles\/s41598-022-17352-3},<br \/>\r\ndoi = {https:\/\/doi.org\/10.1038\/s41598-022-17352-3},<br \/>\r\nyear  = {2022},<br \/>\r\ndate = {2022-08-03},<br \/>\r\njournal = {Scientific Reports},<br \/>\r\nvolume = {12},<br \/>\r\nnumber = {13340},<br \/>\r\nissue = {1},<br \/>\r\npages = {1-12},<br \/>\r\nabstract = {Droplet microfluidics has in recent years found a wide range of analytical and bioanalytical applications. In droplet microfluidics, the samples that are discretized into droplets within the devices are predominantly loaded through tubings, but such tubing-based sample loading has drawbacks such as limited scalability for processing many samples, difficulty for automation, and sample wastage. While advances in autosamplers have alleviated some of these drawbacks, sample loading that can instead obviate tubings offers a potentially promising alternative but has been underexplored. To fill the gap, we introduce herein a droplet device that features a new Tubing Eliminated Sample Loading Interface (TESLI). TESLI integrates a network of programmable pneumatic microvalves that regulate vacuum and pressure sources so that successive sub-microliter samples can be directly spotted onto the open-to-atmosphere TESLI inlet, vacuumed into the device, and pressurized into nanoliter droplets within the device with minimal wastage. The same vacuum and pressure regulation also endows TESLI with cleaning and sample switching capabilities, thus enabling scalable processing of many samples in succession. Moreover, we implement a pair of TESLIs in our device to parallelize and alternate their operation as means to minimizing idle time. For demonstration, we use our device to successively process 44 samples into droplets\u2014a number that can further scale. Our results demonstrate the feasibility of tubing-free sample loading and a promising approach for advancing droplet microfluidics.},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7236','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_abstract\" id=\"tp_abstract_7236\" style=\"display:none;\"><div class=\"tp_abstract_entry\">Droplet microfluidics has in recent years found a wide range of analytical and bioanalytical applications. In droplet microfluidics, the samples that are discretized into droplets within the devices are predominantly loaded through tubings, but such tubing-based sample loading has drawbacks such as limited scalability for processing many samples, difficulty for automation, and sample wastage. While advances in autosamplers have alleviated some of these drawbacks, sample loading that can instead obviate tubings offers a potentially promising alternative but has been underexplored. To fill the gap, we introduce herein a droplet device that features a new Tubing Eliminated Sample Loading Interface (TESLI). TESLI integrates a network of programmable pneumatic microvalves that regulate vacuum and pressure sources so that successive sub-microliter samples can be directly spotted onto the open-to-atmosphere TESLI inlet, vacuumed into the device, and pressurized into nanoliter droplets within the device with minimal wastage. The same vacuum and pressure regulation also endows TESLI with cleaning and sample switching capabilities, thus enabling scalable processing of many samples in succession. Moreover, we implement a pair of TESLIs in our device to parallelize and alternate their operation as means to minimizing idle time. For demonstration, we use our device to successively process 44 samples into droplets\u2014a number that can further scale. Our results demonstrate the feasibility of tubing-free sample loading and a promising approach for advancing droplet microfluidics.<\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7236','tp_abstract')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7236\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/www.nature.com\/articles\/s41598-022-17352-3\" title=\"https:\/\/www.nature.com\/articles\/s41598-022-17352-3\" target=\"_blank\">https:\/\/www.nature.com\/articles\/s41598-022-17352-3<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/https:\/\/doi.org\/10.1038\/s41598-022-17352-3\" title=\"Follow DOI:https:\/\/doi.org\/10.1038\/s41598-022-17352-3\" target=\"_blank\">doi:https:\/\/doi.org\/10.1038\/s41598-022-17352-3<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7236','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">K. Ma; A. Kalra; HL. Tsai; S. Okello; Y. Cheng; SJ. Meltzer; Esophagea Squamous Cell Carcinoma Consortium\r\n<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7276','tp_links')\" style=\"cursor:pointer;\">Accurate Nonendoscopic Detection of Esophageal Squamous Cell Carcinoma Using Methylated DNA Biomarkers<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Gastroenterology, <\/span><span class=\"tp_pub_additional_volume\">vol.  163, <\/span><span class=\"tp_pub_additional_issue\">iss. 2, <\/span><span class=\"tp_pub_additional_pages\">pp. 507-509, <\/span><span class=\"tp_pub_additional_year\">2022<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_abstract_link\"><a id=\"tp_abstract_sh_7276\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7276','tp_abstract')\" title=\"Show abstract\" style=\"cursor:pointer;\">Abstract<\/a><\/span> | <span class=\"tp_resource_link\"><a id=\"tp_links_sh_7276\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7276','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7276\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7276','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7276\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Ma2022,<br \/>\r\ntitle = {Accurate Nonendoscopic Detection of Esophageal Squamous Cell Carcinoma Using Methylated DNA Biomarkers},<br \/>\r\nauthor = {K. Ma and A. Kalra and HL. Tsai and S. Okello and Y. Cheng and SJ. Meltzer and Esophagea Squamous Cell Carcinoma Consortium<br \/>\r\n},<br \/>\r\nurl = {https:\/\/pubmed.ncbi.nlm.nih.gov\/35483446\/},<br \/>\r\ndoi = {DOI: 10.1053\/j.gastro.2022.04.021},<br \/>\r\nyear  = {2022},<br \/>\r\ndate = {2022-08-01},<br \/>\r\njournal = {Gastroenterology},<br \/>\r\nvolume = { 163},<br \/>\r\nissue = {2},<br \/>\r\npages = {507-509},<br \/>\r\nabstract = {Esophageal cancer, with 544,076 deaths in 2020, 1 includes esophageal adenocarcinoma and esophageal squamous cell carcinoma (ESCC). ESCC comprises 90% of esophageal cancers globally. The 5-year survival rate remains poor (15%\u201325% worldwide) because patients usually present with late disease. 2 Although ESCC can be diagnosed by means of esophagogastroduodenoscopy (EGD) with biopsy, EGD is not widely available in low-income countries. 2 Inexpensive, safe, accessible diagnostic alternatives will likely improve diagnosis and outcome.},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7276','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_abstract\" id=\"tp_abstract_7276\" style=\"display:none;\"><div class=\"tp_abstract_entry\">Esophageal cancer, with 544,076 deaths in 2020, 1 includes esophageal adenocarcinoma and esophageal squamous cell carcinoma (ESCC). ESCC comprises 90% of esophageal cancers globally. The 5-year survival rate remains poor (15%\u201325% worldwide) because patients usually present with late disease. 2 Although ESCC can be diagnosed by means of esophagogastroduodenoscopy (EGD) with biopsy, EGD is not widely available in low-income countries. 2 Inexpensive, safe, accessible diagnostic alternatives will likely improve diagnosis and outcome.<\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7276','tp_abstract')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7276\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/35483446\/\" title=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/35483446\/\" target=\"_blank\">https:\/\/pubmed.ncbi.nlm.nih.gov\/35483446\/<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/DOI: 10.1053\/j.gastro.2022.04.021\" title=\"Follow DOI:DOI: 10.1053\/j.gastro.2022.04.021\" target=\"_blank\">doi:DOI: 10.1053\/j.gastro.2022.04.021<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7276','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">F. Shao; P. Lee; H. Li; K. Hsieh; TH. Wang\r\n<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7270','tp_links')\" style=\"cursor:pointer;\">Emerging platforms for high-throughput enzymatic bioassays<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Trends in Biotechnology, <\/span><span class=\"tp_pub_additional_volume\">vol.  41, <\/span><span class=\"tp_pub_additional_issue\">iss. 1, <\/span><span class=\"tp_pub_additional_pages\">pp. 120-133, <\/span><span class=\"tp_pub_additional_year\">2022<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_abstract_link\"><a id=\"tp_abstract_sh_7270\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7270','tp_abstract')\" title=\"Show abstract\" style=\"cursor:pointer;\">Abstract<\/a><\/span> | <span class=\"tp_resource_link\"><a id=\"tp_links_sh_7270\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7270','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7270\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7270','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7270\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Shao2022b,<br \/>\r\ntitle = {Emerging platforms for high-throughput enzymatic bioassays},<br \/>\r\nauthor = {F. Shao and P. Lee and H. Li and K. Hsieh and TH. Wang<br \/>\r\n},<br \/>\r\nurl = {https:\/\/pubmed.ncbi.nlm.nih.gov\/35863950\/},<br \/>\r\ndoi = {DOI: 10.1016\/j.tibtech.2022.06.006},<br \/>\r\nyear  = {2022},<br \/>\r\ndate = {2022-07-18},<br \/>\r\njournal = {Trends in Biotechnology},<br \/>\r\nvolume = { 41},<br \/>\r\nissue = {1},<br \/>\r\npages = {120-133},<br \/>\r\nabstract = {Enzymes have essential roles in catalyzing biological reactions and maintaining metabolic systems. Many in vitro enzymatic bioassays have been developed for use in industrial and research fields, such as cell biology, enzyme engineering, drug screening, and biofuel production. Of note, many of these require the use of high-throughput platforms. Although the microtiter plate remains the standard for high-throughput enzymatic bioassays, microfluidic arrays and droplet microfluidics represent emerging methods. Each has seen significant advances and offers distinct advantages; however, drawbacks in key performance metrics, including reagent consumption, reaction manipulation, reaction recovery, real-time measurement, concentration gradient range, and multiplexity, remain. Herein, we compare recent high-throughput platforms using the aforementioned metrics as criteria and provide insights into remaining challenges and future research trends.},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7270','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_abstract\" id=\"tp_abstract_7270\" style=\"display:none;\"><div class=\"tp_abstract_entry\">Enzymes have essential roles in catalyzing biological reactions and maintaining metabolic systems. Many in vitro enzymatic bioassays have been developed for use in industrial and research fields, such as cell biology, enzyme engineering, drug screening, and biofuel production. Of note, many of these require the use of high-throughput platforms. Although the microtiter plate remains the standard for high-throughput enzymatic bioassays, microfluidic arrays and droplet microfluidics represent emerging methods. Each has seen significant advances and offers distinct advantages; however, drawbacks in key performance metrics, including reagent consumption, reaction manipulation, reaction recovery, real-time measurement, concentration gradient range, and multiplexity, remain. Herein, we compare recent high-throughput platforms using the aforementioned metrics as criteria and provide insights into remaining challenges and future research trends.<\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7270','tp_abstract')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7270\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/35863950\/\" title=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/35863950\/\" target=\"_blank\">https:\/\/pubmed.ncbi.nlm.nih.gov\/35863950\/<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/DOI: 10.1016\/j.tibtech.2022.06.006\" title=\"Follow DOI:DOI: 10.1016\/j.tibtech.2022.06.006\" target=\"_blank\">doi:DOI: 10.1016\/j.tibtech.2022.06.006<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7270','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><div class=\"tp_publication tp_publication_article\"><div class=\"tp_pub_info\"><p class=\"tp_pub_author\">FE. Chen; AY. Trick; AC. Hasnain; K. Hsieh; L. Chen; DJ. Shin; TH. Wang<\/p><p class=\"tp_pub_title\"><a class=\"tp_title_link\" onclick=\"teachpress_pub_showhide('7238','tp_links')\" style=\"cursor:pointer;\">Ratiometric PCR in a Portable Sample-to-Result Device for Broad-Based Pathogen Identification<\/a> <span class=\"tp_pub_type tp_  article\">Journal Article<\/span> <\/p><p class=\"tp_pub_additional\"><span class=\"tp_pub_additional_in\">In: <\/span><span class=\"tp_pub_additional_journal\">Analytical Chemistry, <\/span><span class=\"tp_pub_additional_volume\">vol. 94, <\/span><span class=\"tp_pub_additional_issue\">iss. 26, <\/span><span class=\"tp_pub_additional_pages\">pp. 9372-9379, <\/span><span class=\"tp_pub_additional_year\">2022<\/span>.<\/p><p class=\"tp_pub_menu\"><span class=\"tp_abstract_link\"><a id=\"tp_abstract_sh_7238\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7238','tp_abstract')\" title=\"Show abstract\" style=\"cursor:pointer;\">Abstract<\/a><\/span> | <span class=\"tp_resource_link\"><a id=\"tp_links_sh_7238\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7238','tp_links')\" title=\"Show links and resources\" style=\"cursor:pointer;\">Links<\/a><\/span> | <span class=\"tp_bibtex_link\"><a id=\"tp_bibtex_sh_7238\" class=\"tp_show\" onclick=\"teachpress_pub_showhide('7238','tp_bibtex')\" title=\"Show BibTeX entry\" style=\"cursor:pointer;\">BibTeX<\/a><\/span><\/p><div class=\"tp_bibtex\" id=\"tp_bibtex_7238\" style=\"display:none;\"><div class=\"tp_bibtex_entry\"><pre>@article{Chen2022,<br \/>\r\ntitle = {Ratiometric PCR in a Portable Sample-to-Result Device for Broad-Based Pathogen Identification},<br \/>\r\nauthor = {FE. Chen and AY. Trick and AC. Hasnain and K. Hsieh and L. Chen and DJ. Shin and TH. Wang},<br \/>\r\nurl = {https:\/\/pubs.acs.org\/doi\/full\/10.1021\/acs.analchem.2c01357},<br \/>\r\ndoi = {https:\/\/doi.org\/10.1021\/acs.analchem.2c01357},<br \/>\r\nyear  = {2022},<br \/>\r\ndate = {2022-06-21},<br \/>\r\nurldate = {2022-06-21},<br \/>\r\njournal = {Analytical Chemistry},<br \/>\r\nvolume = {94},<br \/>\r\nissue = {26},<br \/>\r\npages = {9372-9379},<br \/>\r\nabstract = {Polymerase chain reaction (PCR)-based diagnostic testing is the gold standard method for pathogen identification (ID) with recent developments enabling automated PCR tests for point-of-care (POC) use. However, multiplexed identification of several pathogens in PCR assays typically requires optics for an equivalent number of fluorescence channels, increasing instrumentation\u2019s complexity and cost. In this study, we first developed ratiometric PCR that surpassed one target per color barrier to allow multiplexed identification while minimizing optical components for affordable POC use. We realized it by amplifying pathogenic targets with fluorescently labeled hydrolysis probes with a specific ratio of red-to-green fluorophores for each bacterial species. We then coupled ratiometric PCR and automated magnetic beads-based sample preparation within a thermoplastic cartridge and a portable droplet magnetofluidic platform. We named the integrated workflow POC-ratioPCR. We demonstrated that the POC-ratioPCR could detect one out of six bacterial targets related to urinary tract infections (UTIs) in a single reaction using only two-color channels. We further evaluated POC-ratioPCR using mock bacterial urine samples spiked with good agreement. The POC-ratioPCR presents a simple and effective method for enabling broad-based POC PCR identification of pathogens directly from crude biosamples with low optical instrumentation complexity.},<br \/>\r\nkeywords = {},<br \/>\r\npubstate = {published},<br \/>\r\ntppubtype = {article}<br \/>\r\n}<br \/>\r\n<\/pre><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7238','tp_bibtex')\">Close<\/a><\/p><\/div><div class=\"tp_abstract\" id=\"tp_abstract_7238\" style=\"display:none;\"><div class=\"tp_abstract_entry\">Polymerase chain reaction (PCR)-based diagnostic testing is the gold standard method for pathogen identification (ID) with recent developments enabling automated PCR tests for point-of-care (POC) use. However, multiplexed identification of several pathogens in PCR assays typically requires optics for an equivalent number of fluorescence channels, increasing instrumentation\u2019s complexity and cost. In this study, we first developed ratiometric PCR that surpassed one target per color barrier to allow multiplexed identification while minimizing optical components for affordable POC use. We realized it by amplifying pathogenic targets with fluorescently labeled hydrolysis probes with a specific ratio of red-to-green fluorophores for each bacterial species. We then coupled ratiometric PCR and automated magnetic beads-based sample preparation within a thermoplastic cartridge and a portable droplet magnetofluidic platform. We named the integrated workflow POC-ratioPCR. We demonstrated that the POC-ratioPCR could detect one out of six bacterial targets related to urinary tract infections (UTIs) in a single reaction using only two-color channels. We further evaluated POC-ratioPCR using mock bacterial urine samples spiked with good agreement. The POC-ratioPCR presents a simple and effective method for enabling broad-based POC PCR identification of pathogens directly from crude biosamples with low optical instrumentation complexity.<\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7238','tp_abstract')\">Close<\/a><\/p><\/div><div class=\"tp_links\" id=\"tp_links_7238\" style=\"display:none;\"><div class=\"tp_links_entry\"><ul class=\"tp_pub_list\"><li><i class=\"fas fa-globe\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/pubs.acs.org\/doi\/full\/10.1021\/acs.analchem.2c01357\" title=\"https:\/\/pubs.acs.org\/doi\/full\/10.1021\/acs.analchem.2c01357\" target=\"_blank\">https:\/\/pubs.acs.org\/doi\/full\/10.1021\/acs.analchem.2c01357<\/a><\/li><li><i class=\"ai ai-doi\"><\/i><a class=\"tp_pub_list\" href=\"https:\/\/dx.doi.org\/https:\/\/doi.org\/10.1021\/acs.analchem.2c01357\" title=\"Follow DOI:https:\/\/doi.org\/10.1021\/acs.analchem.2c01357\" target=\"_blank\">doi:https:\/\/doi.org\/10.1021\/acs.analchem.2c01357<\/a><\/li><\/ul><\/div><p class=\"tp_close_menu\"><a class=\"tp_close\" onclick=\"teachpress_pub_showhide('7238','tp_links')\">Close<\/a><\/p><\/div><\/div><\/div><\/div><div class=\"tablenav\"><div class=\"tablenav-pages\"><span class=\"displaying-num\">212 entries<\/span> <a class=\"page-numbers button disabled\">&laquo;<\/a> <a class=\"page-numbers button disabled\">&lsaquo;<\/a> 1 of 6 <a href=\"https:\/\/me.jhu.edu\/thwang\/publications.html?limit=2&amp;tgid=&amp;yr=&amp;type=&amp;usr=&amp;auth=&amp;tsr=#tppubs\" title=\"next page\" class=\"page-numbers button\">&rsaquo;<\/a> <a href=\"https:\/\/me.jhu.edu\/thwang\/publications.html?limit=6&amp;tgid=&amp;yr=&amp;type=&amp;usr=&amp;auth=&amp;tsr=#tppubs\" title=\"last page\" class=\"page-numbers button\">&raquo;<\/a> <\/div><\/div><\/div><br \/>\n<a href=\"#top\">Top<\/a><\/p>\n<h3>Patents<\/h3>\n<ol>\n<li>H. Wang, F.M. Friedrich, J.M. Burke, and K. Liu \u201cConcentration and Accumulation of Target Species in Response to a Gradient of Solute or Solvent\u201d (JHU Ref. C13791; US provisional patent)<\/li>\n<li>H. Wang, K. Hsieh, H. Zec, L. Liu, A.M. Kaushik, Y. Yun, \u201cMultiplexed, Continuous-flow, droplet-based platform for high-throughput genetic detection\u201d (JHU Ref. C13529; US provisional patent)<\/li>\n<li>H. Wang, T.D. Rane, H.C Zec, \u201cSystem and device for high throughput generation of combinatorial droplets and methods of use\u201d \u00a0(PCT\/US2015\/012927; JHU Ref. C12862)<\/li>\n<li>H. Wang, D.J. Shin, \u201cSelf-contained cartridge and methods for integrated biochemical assay at the point-of-care\u201d (JHU Ref. C12720)<\/li>\n<li>-H. Wang, S. Yang, M.A. Jacobs, P. Athamanolap, S.I. Fraley, V. Agarwal, V. Parekh. \u201cMelt curve classifier for reliable large-scale genotyping of sequence variants\u201d JHU Case Number C12600, provisional patent<\/li>\n<li>Stephanie I. Fraley, T.-H. Wang, and S. Yang. \u201cA novel technology for broad-based, yet single molecule sensitive profiling in heterogeneous biological samples.\u201d JHU Case Number C12403, provisional patent<\/li>\n<li>H. Wang, Y. Zhang, \u201cFabrication of Hierarchical Silica Nanomembrane using Heat-Shrinking Polymers and Applications of Silica Nanomembrane for the Solid Phase Extraction of Nucleic Acids\u201d, JHU Case Number C12404, provisional patent<\/li>\n<li>H. Wang, Chi-Hang Chiou, Dong Jin Shin , \u201cElectromagnetically Actuated Droplet Microfluidic Chip and System\u201d (JHU Ref. C12262)<\/li>\n<li>H. Wang and Y. Zhang, \u201cSurface Energy Traps (SETs) Enabled Complex Droplet Manipulation\u201d (JHU Ref. C11971)<\/li>\n<li>H. Wang, K. Liu and Y. Song, \u201cmiRNA Analysis Method\u201d (US Provisional US 61\/598513, JHU Ref. C11885)<\/li>\n<li>H. Wang, T.D. Rane, H. C. Zec and W.Ch. Chu \u201cSystems and methods for screening a library of samples\u201d (US13\/708,510. JHU Ref. C11803)<\/li>\n<li>Yang, T.H. Wang, S.K. Park and Y Zhang, \u201cMethod and apparatus for continuous microfluidic sample separation and concentration using AC electric field\u201d (JHU Ref. C11562)<\/li>\n<li>H. Wang, Y. Zhang, S. Park and S. Yang, \u201cSelf-sustained Droplet Microfluidic Chip and System for Integrated Sample Preparation and Nucleic Acids Detection\u201d (PCT\/US2011\/045363, JHU Ref. C11183)<\/li>\n<li>H. Wang, H.Q. Mao, W. Beh and D. Kraitchman, \u201cKilohertz Generation of Monodisperse Microbeads on a Polymeric Membrane Microfluidic Pseudocheck Valve Device\u201d (PCT\/US2011\/054598, JHU Ref. C11248)<\/li>\n<li>Ahuja, V. Bailey, S.B. Baylin, J.G. Herman, T.H. Wang, J. Yi, \u201cEarly Detection of DNA Methylation Biomarker in Cancer Patient Sera\u201d (PCT\/US2011\/037926, JHU Ref. C11091)<\/li>\n<li>H. Wang and K. Liu, \u201cHydrodynamic Particle Separation and Detection Systems and Methods\u201d, (PCT\/US2011\/056941, JHU Ref. C11263)<\/li>\n<li>H. Wang and K.J. Liu, &#8220;Single Molecule Spectroscopy for Analysis of Cell-free Nucleic Acid Biomarkers&#8221; (PCT\/US2010\/033888, JHU Ref. C10750)<\/li>\n<li>H. Wang and Y. Zhang, &#8221; Device and Method of Preparing and Performing Multiple Polymerase Chain Reactions&#8221; (US 12\/716,031, JHU Ref. C10659)<\/li>\n<li>H. Wang, S. Baylin, J. Herman, H. Easwaran and H. Carraway, \u201cCompositions and Methods for Polynucleotide Extraction and Methylation Detection\u201d (PCT\/US2009\/000039, JHU Ref. C10249)<\/li>\n<li>M. Ho and T.H. Wang, \u201cBiosensors and Methods for Their Use\u201d (PCT\/US2001\/025444)<\/li>\n<li>H. Wang, K.J. Liu, and I.M. Shih, &#8221; DNA Integrity Assay (DIA) for Cancer Diagnostics, Using Confocal Fluorescence Spectroscopy&#8221; (US8,835,110, JHU Ref. C10534)<\/li>\n<li>H. Wang and K. Liu, \u201cCylindrical Illumination Confocal Spectroscopy System\u201d (US8,248,609, JHU Ref. C10398)<\/li>\n<li>H. Wang, K.J. Liu, C.M. Puleo and T. Rane, &#8221; Microfluidic System for High-Throughput, Droplet-based, Single Molecule Analysis with Low Reagent Consumption&#8221; (US9,284,601, issued on 03\/15\/2016, JHU Ref. C10662)<\/li>\n<li>F. Miller, J. Huang, T.H. Wang, C.M. Ho and M. Liu, \u201cElectrochemical Detection of Mismatch in Nucleic Acids\u201d (US 7,291,457)<\/li>\n<li>H. Wang, \u201cMethod for determining standard cycle time of a stage dynamically\u201d (US5,825,650)<\/li>\n<li>A. Hulbert, J. Herman, M. Brock, T.H. Wang, and A. Stark &#8220;Early detection of Individuals at High Risk for Lung Cancer of all Stages Using Gene promoter Methylation in Plasma and Sputum&#8221; JHU C13599<\/li>\n<\/ol>\n<p><a href=\"#top\">Top<\/a><\/p>\n<h3>Conference Publications<\/h3>\n<ol>\n<li>J. Shin, P. Athamanolap, L.Chen, J. Hardick, CA Gaydos and T.H. Wang, \u201cA Smartphone-Based Mobile NAAT Diagnostic Suite for Chlamydia Detection\u201d, <em>Proc. 26<sup>th<\/sup> Anniversary World Congress on Biosensors (Biosensors 2016)<\/em>, (accepted)<\/li>\n<li>M. Friedrich, J.M. Burke, K.J. Liu, T.H. Wang, \u201cIn-line Preconcentration, Size Separation, and Single-Molecule Detection Without Applied Electric Fields, <em>Proc. 29th IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2016)<\/em>, p. 181-184, 2016<\/li>\n<li>J. Shin, L. Chen and T.H. Wang, \u201cSingle-bacteria Confocal Spectroscopy: An Ultrasensitive method for Real-time Monitoring of Bacterial Growth\u201d, <em>Proc. 19th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2015)<\/em>, p. 340-342, 2015<\/li>\n<li>Kaushik, K. Hsieh, L. Chen, D.J. Shin and T.H. Wang, \u201cRapid Assessment of Bacterial Vitality and Antibiotic Susceptibility via High-Throughput Picoliter-Droplet Single-Cell Assay\u201d, <em>Proc. 19th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2015)<\/em>, p. 531-533, 2015<\/li>\n<li>Liu, K. Hsieh, A. Kaushik, H.C. Zec and T.H. Wang, \u201cMultiplexed, Continuous-Flow, Droplet-Based PCR Genotyping Platform for High-Throughput Agriculture Marker Assisted Selection\u201d, <em>Proc. 19th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2015)<\/em>, p. 1368-1370, 2015<\/li>\n<li>Zec, C. O\u2019Keefe, P. Ma, T.H. Wang, \u201cUltra-Thin, Evaporation-Resistant PDMS Devices for Absolute Quantification of DNA Using Digital PCR\u201d, <em>The 18th International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers 2015)<\/em>, p.536-539, 2015<\/li>\n<li>Guan, L. Chen, T. Rane, A. Kaushik, T.H. Wang, \u201cDigital Droplet ELOHA For Nucleic Molecule Counting And Analysis\u201d, <em>The 18th International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers 2015)<\/em>, p.536-5460-463, 2015<\/li>\n<li>C. Zec, C.J. Glover, W. Hsieh, L. Liu, C. O\u2019Keefe and T.H. Wang, \u201cMethods for Controlling Water Evaporation in PDMS-Based Microfluidic Devices\u201d, <em>Proc. 18th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2014), <\/em>p. 1743-1745, 2014<\/li>\n<li>J. Shin, P. Athamanonolap, L. Chen and T.H. Wang, \u201cIntegrated droplet microfluidic platform for nucleic acids amplification test of Chlamydia trachomatis infection\u201d, Proc. 24<sup>th<\/sup> Anniversary World Congress on Biosensors (Biosensors 2014)<\/li>\n<li>C. Zec, T.D. Rane, P. Ma and T.H. Wang, \u201cParallelization of Fission and Fusion- Operations for High Throughput Generation of Combinatorial Droplets\u201d, <em>Proc. 27th IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2014)<\/em>, p. 334-337, 2014<\/li>\n<li>J. Shin, L. Chen and T.H. Wang, \u201cA Simple Integrated Diagnostic Platform for DNA Testing of Chlamydia Trachomatis Infection, \u201c <em>Proc. 17th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2013)<\/em>, p. 1350-1352, 2013<\/li>\n<li>M. Friedrich, K.J. Liu and T.H. Wang, \u201c Single Molecule Hydrodynamic Separation for Ultrasensitive and Quantitative DNA Size Separations,\u201d <em>Proc. 17th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2013)<\/em>, p. 35-37, 2013<\/li>\n<li>D. Rane, H.C. Zec and T.H. Wang, \u201cA Multiplexed Microfluidic Droplet Platform for Matrix Metalloproteinase Screening\u201d, <em>Proc. 17th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2013)<\/em>, p. 1595-1597, 2013<\/li>\n<li>K. Wu, S. F. Friedrich, K.J. Liu and T.H. Wang, \u201c Chip-Based DNA Separation in Free Solution by Inertial Hydrodynamic Forces\u201d, <em>Proc. 17th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2013)<\/em>, p. 578-580, 2013<\/li>\n<li>J. Shin, A. Stark and T.H. Wang, \u201c Droplet Bisulfite Conversion Platform for Epigenetic Cancer Biomarker Detection\u201d, <em>The 17<sup>th<\/sup> International Conference on Solid-State Sensors, Actuators and Microsystems(Transducers 2013)<\/em>, p.2181-2184, 2013<\/li>\n<li>Zhang, Y. Zhang, B. Keeley, A. Stark and T.H. Wang, \u201cSpontaneous Shrinking Silica Nanomembrane for Solid Phase\u201d, <em>Proc. 8th Annual IEEE International Conference on Nano\/Micro Engineered and Molecular Systems <\/em>(<em>IEEE NEMS 2013<\/em>), p. 444-445, 2013<\/li>\n<li>Athamanolap, B. Keeley, D.J. Shin and T.H. Wang, \u201cQuantitative Analysis of DNA Methylation Based on Melting Curve Analysis\u201d, <em>Proc. 8th Annual IEEE International Conference on Nano\/Micro Engineered and Molecular Systems<\/em> (<em>IEEE NEMS 2013<\/em>), p.1116-1118, 2013<\/li>\n<li>Zhang, Y Zhang, T.H. Wang, \u201cHierarchical Silica Nanomembrane Driven by Thermal Shrinkage and its Application for Solid Phase DNA Extraction\u201d, <em>Proc. The 13th IEEE International Conference on Nanotechnology (IEEE NANO 2013)<\/em>, 2013<\/li>\n<li>C. Zec, T.D. Rane, W.C. Chu, V. Wang and T.H. Wang, \u201c A Microfluidic Droplet Platform for Multiplexed Single Nucleotide Polymorphism Analysis of an Array Plant Genomic DNA Samples\u201d, <em>Proc. 26th IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2013)<\/em>, p.263-266, 2013<\/li>\n<li>H. Chiou, D.J. Shin, S. Hosmane, Y Zhang and T.H. Wang, \u201cElectromagnet-Actuated Droplet Platform for Sample-to-Answer Genetic Detection\u201d, <em>Proc. 26th IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2013)<\/em>, p.98-101, 2013<\/li>\n<li>Zhang and T.H. Wang, \u201c All-in-One Droplet Platform for Multiplexed Genetic Detection in Blood\u201d<em> Proc. 26th IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2013)<\/em>, p. 1061-1064, 2013<\/li>\n<li>Zhang and T.H. Wang, \u201cFlip-Drop: Droplet Array Created by Surface Energy Trap for Combinatorial Screening,\u201d <em>Proc. 26th IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2013)<\/em>, p.1149-1152, 2013<\/li>\n<li>W. Beh, C. Weiss, H.Q. Mao, D. L. Kraitchman, and T.H. Wang, \u201cHigh-Throughput Microfluidic Preparation of Imaging-Visible Embolic Beads\u201d, <em>Proc. EMBS Micro and Nanotechnology in Medicine Conference<\/em>, p.59, 2012<\/li>\n<li>Zhang, Y. Zhang, B. Keeley, A. Stark and T.H. Wang, \u201cFabricating and Applying Hierarchical Silica Nanomembrane with for Solid Phase DNA Extraction\u201d <em>The 6th IEEE International Conference on Nano\/Molecular Medicine and Engineering (IEEE NANOMED 2012)<\/em>, 2012<\/li>\n<li>Keeley, Yi Zhang, Ye Zhang, A. Stark, T.H. Wang, \u201cQuantum Dot FRET Linker Probes for Highly Sensitive DNA Methylation Detection\u201d, <em>Proc. IEEE 12<sup>th<\/sup> International Conference on Nanotechnology (IEEE NANO 2012)<\/em>, 7848, p.1-4, 2012<\/li>\n<li>Zhang and T.H. Wang, \u201cQuantum Dot Electrophoretic Mobility Shift Assay and Its Application to the Measurement of Exonuclease Activity\u201d <em>Proc. IEEE 12<sup>th<\/sup> International Conference on Nanotechnology (IEEE NANO 2012)<\/em>, 7685, p.1-4, 2012<\/li>\n<li>Song, L. Zhang, M. Chen and T.H. Wang, \u201cSingle Quantum Dot Fluorescence Enhancement by Tunable Nanoporous Gold\u201d, <em>Proc. IEEE 12<sup>th<\/sup> International Conference on Nanotechnology (IEEE NANO 2012)<\/em>, 7877, p.1-4, 2012<\/li>\n<li>Zhang and T.H. Wang, \u201cDroplet Immobilization, Splitting, Metering and Aliquoting with Surface Energy Traps Created Using SU8 Shadow Mask\u201d <em>Proc. 16th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2012)<\/em>, p. 73-75, 2012<\/li>\n<li>Zhang and T.H. Wang, \u201cSurface Energy Trap Assisted Rapid Serial Dilution on Droplet Platform for Bacteria Antibiotics Susceptibility Test\u201d <em>Proc. 16th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2012)<\/em>, p. 467-469, 2012<\/li>\n<li>J. Liu, T.D. Rane, Y. Zhang, C. Beh, D.J. Shin, T.H. Wang, \u201cAn Integrated Platform for Single Molecule Free Solution Hydrodynamic Separation Using Yoctomoles of DNA and Picoliter Samples\u201d, <em>ASME 10th International Conference on Nanochannels, Microchannels and Minichannels (ICNMM 2012)<\/em>, ICNMM2012-73154 (p.1-6), 2012<\/li>\n<li>Zec, T.D. Rane, W.C. Chu and T.H. Wang, \u201cMultiplexed Screening of a Large Library of Biological Samples through on-Demand Droplet Generation and Fusion\u201d, <em>ASME 2012 10th International Conference on Nanochannels, Microchannels and Minichannels (ICNMM 2012)<\/em>, ICNMM2012-73159 (p.1-6), 2012<\/li>\n<li>Song, Y. Zhang, and T.H. Wang, \u201cSingle Quantum Dot-Based Multiplexed Point Mutation Detection by Gap Ligase Chain Reaction\u201d, <em>Proc. 15th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2011)<\/em>, p.1779-1781, 2011<\/li>\n<li>Zhang, D.J. Shin and T.H. Wang, \u201cDetecting Genetic Variations in A Droplet\u201d, <em>Proc. 15th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2011)<\/em>, p.1179-1181, 2011<\/li>\n<li>J. Liu and T.H. Wang, \u201c PCR-free, microfluidic single molecule analysis of circulating nucleic acids in lung cancer patient serum\u201d, <em>Proc. 33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC \u201911)<\/em>, p.8392-8395, 2011<\/li>\n<li>Zhang and T.H. Wang, \u201c An Active Gyroscopic Magnetic Micromixer for Rapid Fluid Mixing in Droplet Based Microfluidic Systems\u201d, <em>The 16<sup>th<\/sup> International Conference on Solid-State Sensors, Actuators and Microsystems(Transducers 2011)<\/em>, p.1769-1772, 2011<\/li>\n<li>Zhang, S.K. Park, S. Yang and T.H. Wang, \u201cFully Integrated Droplet Based Point-of-Care Platform for Molecular Detection from Crude Biosamples\u201d, <em>The 16<sup>th<\/sup> International Conference on Solid-State Sensors, Actuators and Microsystems(Transducers 2011),<\/em> p.1927-1930, 2011<\/li>\n<li>H. Wang, V. Bailey and K. Liu, \u201cQuantum Dots and Microfluidic Single Molecule Detection for Screening Genetic and Epigenetic Cancer Markers in Clinical Samples\u201d, <em>Proc. 2011 SPIE Defense, Security and Sensing Conference<\/em>, Volume 8031, P. 80311W<\/li>\n<li>D. Rane, H. Zec, C.M. Puleo, A.P. Lee and T.H. Wang, \u201cHigh-Throughput Single-Cell Pathogen Detection on a Droplet Microfluidic Platform\u201d, <em>Proc. 24th IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2011)<\/em>, p.881-884, 2011<\/li>\n<li>Stark, Y. Zhang, V. Bailey, B. Keeley. T.H. Wang, &#8220;Increasing Throughput and Sensitivity of DNA Methylation Analysis through Functional Nanoparticles&#8221; The 6th Annual IEEE International Conference on Nano\/Micro Engineered and Molecular Systems (<em>IEEE NEMS 2011<\/em>), 1091-1094, 2011<\/li>\n<li>Y Zhang and T.H. Wang, &#8220;A Quantum Dot Based Electrophoretic Mobility Shift Assay for High Resolution Copy Number Variation Study&#8221;, <em>The 6th Annual IEEE International Conference on Nano\/Micro Engineered and Molecular Systems (IEEE NEMS 2011)<\/em>, p.841-844, 2011<\/li>\n<li>Zhang, I.M. Shih, T.L. Wang and T.H. Wang, &#8221; A Quantum Dot Based Nanoassay for Quantifying Gene Copy Number with Ultrahigh Resolution&#8221;, <em>Proc. 14th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2010)<\/em>, p.1154-1156, 2010<\/li>\n<li>W. Beh, W. Zhou and T.H. Wang, &#8220;Oxygen Plasma-Free Microfluidic Device Sealing&#8221;, <em>Proc. 14th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2010)<\/em> p.1217-1219, 2010<\/li>\n<li>W. Beh, D. Kraitchman, H.Q. Mao, T.H. Wang, &#8220;High-throughput Monodisperse Alginate Gel Bead Formation using Microfluidic Pseudo-Check Valve&#8221;, <em>Proc. 14th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2010)<\/em> p.425-427, 2010<\/li>\n<li>Zhang and T.H. Wang, \u201cAn Automated All-in-one Microfluidic Device for Parallel Solid Phase DNA Extraction and Droplet-in-Oil PCR Analysis\u201d, <em>Proc. 23rd IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2010)<\/em>, P.971-974, 2010<\/li>\n<li>Zhang and T.H. Wang, \u201cGeomorphology-Assisted Manopulation of Magnet-Actuated Droplet for Solid Phase DNA Extraction and Droplet-in-Oil PCR\u201d, <em>Proc. 23rd IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2010)<\/em>, p.1047-1050, 2010<\/li>\n<li>D. Rane, C.M. Puleo, H. Zec, Y. Zhang, A.P. Lee and T.H. Wamh, \u201cAnalyte Detection in Droplets: One Molecule at a Time\u201d, <em>Proc. 13th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2009)<\/em>, p.15-17, 2009<\/li>\n<li>M. Puleo, H.C. Zec, Y. Sung and T.H. Wang, \u201cMicro-evaporator as Interconnects to Low-Volume Microfluidic Components\u201d, <em>Proc. 13th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2009)<\/em>, p.932-935, 2009<\/li>\n<li>J. Bailey, C.M. Puleo, Y.P. Ho, H.C. Yeh, T.H. Wang, &#8220;Quantum Dots in Molecular Detection of Diseases&#8221;, <em>31st Annual International Conference on the IEEE Engineering in Medicine and Biology Society ( IEEE EMBC 2009)<\/em>, p. 4089-4092, 2009<\/li>\n<li>Zhang, V. Bailey, C.M. Puleo, H. Easwaran, E. Griffiths, J.G. Herman, S.B. Baylin, T.H. Wang, \u201cHigh Throughput DNA Methylation Analysis on a Droplet-in-Oil Polymerase Chain Reaction Array\u201d, <em>The 15<sup>th<\/sup> International Conference on Solid-State Sensors, Actuators and Microsystems(Transducers 2009)<\/em>, p.806-808, 2009<\/li>\n<li>Zhang, V. Bailey, C.M. Puleo, C. Chen and T.H. Wang, \u201cMultiple gene Analysis within a Simple Droplet-in-Oil Microfluidic PCR Platform\u201d, Proc. 12th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (<em>micro-TAS 2008<\/em>), p. 751-753 , 2008<\/li>\n<li>P. Ho, H.H. Chen, K. Leong and T.H. Wang \u201cQuantitative Kinetic Analysis of DNA Nanocomplex Self-Assembly with Quantum Dots FRET in a Microfluidic Device\u201d, <em>Proc. 21st IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2008)<\/em>, p. 30-33, 2008<\/li>\n<li>M. Puleo, H.C. Yeh and T.H. Wang \u201cSingle Molecule Detection in Truly, Nanoliter-Sized Volumes: Coupling Evaporation-Based, Microfluidic Concentration with Confocal Fluorescence Spectroscopy\u201d <em>Proc. 21st IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS 2008)<\/em>, p.200-203, 2008<\/li>\n<li>Liu and T.H. Wang, \u201cQuantitative Confocal Spectroscopy \u2013 Rectifying the Limitations of Single Molecule Detection\u201d, <em>Proc. 3<sup>rd<\/sup> Annual IEEE International Conferences on Nano\/Micro Engineered and Molecular Systems (IEEE-NEMS 2008)<\/em>, p. 1189-1192, 2008<\/li>\n<li>C. Yeh. Y.P. Ho, C.M. Puleo and T.H. Wang,\u201d Towards single-molecule diagnostics using microfluidic manipulation and quantum dot nanosensors, <em>Proc. 5<sup>th<\/sup> International Conference on Nanochannels, Microchannels and Minichannels<\/em>, (<em>ICNMM 2007<\/em>), p. 1133-1140, 2007<\/li>\n<li>P. Ho and T.H. Wang, \u201cMultiplexed Detection of Anthrax Sequences with Quantum Dot Nanoprobes\u201d, <em>Proc. IEEE\/NLM Life Science Systems and Application Workshop<\/em>, p. 62-63, 2006<\/li>\n<li>Murray , K. Rebello, J. Crookston, J. Miragliotta and T.H. Wang, \u201cHigh-degree Concentration of Bio-agents Using Electrokinetic Manipulations\u201d, <em>Proc. IEEE\/NLM Life Science Systems and Application Workshop<\/em>, p. 94-95, 2006<\/li>\n<li>P. Ho, M.C. Kung and T.H. Wang, &#8221; Separation-free Detection of Low-abundant Biomolecules with Two-Color Colocalization of Quantum Dot Probes,&#8221; <em>Proc. 9th International Conference on Miniaturized Chemical and Biochemical Analysis Systems (micro-TAS 2005)<\/em>, p. 1330-1332, 2005<\/li>\n<li>C. Yeh, Y.P. Ho and T.H. Wang, &#8220;Quantum Dot-Mediated Separation-Free Assay for Point Mutation Detection &#8221; <em>Proc. NSTI- Bio-Nanotechnology Conference<\/em>, 198-201, 2005<\/li>\n<li>C. Yeh, E. Simone, C.Y. Zhang and T.H. Wang, \u201cSingle Bio-Molecule Detection with Quantum-dots in Flow-rate Controlled Microchannel\u201d, <em>Proc. 17h IEEE Annual Workshop of Micro Electro Mechanical Systems (IEEE MEMS 2004)<\/em>, p 371-374, 2004<\/li>\n<li>Y. Chao, C.Y. Zhang and T.H. Wang,\u201d Measurement of in-situ Flow Velocity Using Single-Molecule Detection for the Application of Biomolecule Quantification\u201d, <em>Proc.<\/em><em> Hilton Head 2004<\/em><em> Solid-State Sensor, Actuator, and Microsystems Workshop (Hilton Head 2004)<\/em>, p. 176-179, 2004<\/li>\n<li>H. Wang and C.M. Ho, \u201cNano\/micro Te<\/li>\n<li>chnologies for Single Molecule Manipulation and Detection\u201d, <em> IEEE International Conference on Robotics and Control (IEEE ICRA 2003)<\/em>, vol. 3, p. 3630-3635, 2003<\/li>\n<li>K. Wong, C.Y. Chen, T.H. Wang and C.M. Ho, &#8220;An AC Electroosmotic Processor for Biomolecules,&#8221; Proc. 12<sup>th<\/sup> International Conference on Solid-state Sensors, Actuators, and Microsystems (<em>Transducers 2003<\/em>), vol. 1, p. 20-23, 2003<\/li>\n<li>K. Wong, T.H. Wang and C.M. Ho, \u201cOptical Fiber Tip Fabricated by Surface Tension Controlled Etching\u201d, <em>Proc. Hilton Head 2002: Solid-State Sensor, Actuator, and Microsystems Workshop (Hilton Head 2002)<\/em>, p. 94-97, 2002<\/li>\n<li>H. Wang, P.K. Wong and C.M. Ho, \u201cElectrical Molecular Focusing for Laser Induced Fluorescence Based Single DNA Detection\u201d, <em>Proc. 15<sup>th<\/sup> IEEE International Conference on Micro Electro Mechanical Systems(IEEE MEMS 2002)<\/em>, p. 15-18, 2002<\/li>\n<li>H. Wang, S. Masset and C.M. Ho, &#8220;A Zepto Mole DNA Micro Sensor,&#8221; <em>Proc. 14<sup>th<\/sup> IEEE International Conference on Micro Electro Mechanical Systems (IEEE MEMS)<\/em>,\u00a0 p. 431-434, 2001<\/li>\n<li>H. Wang, S. Masset and C.M. Ho &#8220;Molecular Beacon Based Biological Detection System&#8221;, <em>Proc. The International Conference on Mathematics and Engineering Techniques in Medicine and Biological Science<\/em>, p. 295-300, 2000<\/li>\n<li>H. Wang, K.C. Lin and S.R. Huang \u201cMethod of Dynamically Determining Cycle Time of a Working Stage\u201d, <em>Proc. 21<sup>st<\/sup> IEEE\/CPMT International Electronics manufacturing Technology Symposium<\/em>, p. 403-407, 1997<\/li>\n<li>C. Chang, W.L. Jan. T.H. Wang and C.S. Chang, \u201cAnalysis of Proportional Machine Allocation in a Deterministic Re-entrant Line\u201d, <em>Proc.<\/em><em> INRIA\/IEEE Conference on Emerging Technology and Factory Automation<\/em>, vol. 2, p. 1-5, 1995<\/li>\n<\/ol>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Journal Publications | Patents | Conference Publications Journal Publications Top Patents H. Wang, F.M. Friedrich, J.M. Burke, and K. Liu \u201cConcentration and Accumulation of Target Species in Response to a Gradient of Solute or Solvent\u201d (JHU Ref. C13791; US provisional patent) H. Wang, K. Hsieh, H. Zec, L. Liu, A.M. Kaushik, Y. Yun, \u201cMultiplexed, Continuous-flow, [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-11","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/me.jhu.edu\/thwang\/wp-json\/wp\/v2\/pages\/11","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/me.jhu.edu\/thwang\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/me.jhu.edu\/thwang\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/me.jhu.edu\/thwang\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/me.jhu.edu\/thwang\/wp-json\/wp\/v2\/comments?post=11"}],"version-history":[{"count":53,"href":"https:\/\/me.jhu.edu\/thwang\/wp-json\/wp\/v2\/pages\/11\/revisions"}],"predecessor-version":[{"id":990,"href":"https:\/\/me.jhu.edu\/thwang\/wp-json\/wp\/v2\/pages\/11\/revisions\/990"}],"wp:attachment":[{"href":"https:\/\/me.jhu.edu\/thwang\/wp-json\/wp\/v2\/media?parent=11"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}