LOCATION OF ANALYSIS FILES AND RAW DATA:

All matlab analysis files and some sample data are located on the Mac Server under: Groups/Human Skin papers/ SkinAnalysisFiles_Procedures_and_SampleData.

Full data from the Acta Biomaterialia papers are currently stored on the Lab PC Storage System under the folder 'Theresa'.

STRESS/STRAIN DATA ANALYSIS

1. Copy Image, Time, and Pressure from node data and test files into excel. Convert Pressure from psi to kPa. Input Radius (37.5 mm) and initial thickness.

2. Identify image with max pressure for all 3 loadings, and end of unloading for all 3.

3. Load image into ‘Nov10EllipsoidFit.m’ and fit to general ellipse (0)

4. Use eigenvectors to determine counter-clockwise rotation angle needed to align stiffest/fiber direction (b) with x-axis.

5. Run ‘Nov10EllipsoidFit.m’ for all images on loading curve. Copy a, b, and c into excel file.

6. Input angle into ‘Jun16FittedStrains.m’. Run for max pressure image. Check contours (esp. W over distorted, U and V) to make sure everything is aligned properly. (W oval should be vertical, U and V should not be rotated/skewed). Check that strains are calculated at (0,0,Z) àcheck that Xv(1)=Yh(1)=0, and that Xv(centerH)=Yh(centerV)=0. Adjust if necessary and update in “Jun16FittedStrainsGrid.m” as well.

7. Run “Jun16FittedStrainsGrid.m” for all images on loading /unloading curve for all 3 load/unload cycles (including angle as an input).

8. Copy resulting strains into excel file and compute associated stretches [stretch = sqrt(2*strain+1)]

9. Calculate deformed thickness [original thickness/(StretchF*StretchP)]

10. Calculate StressFiber (circ.) and StressPerp(long,).

11. Determine baseline (i.e. where stress and strain pick up). Zero all subsequent pressures accordininly and only plot stress/strain for those points.

12. QUICK PRECONDITIONING CHECK: Identify beginning of each 3 loading cycles input into ‘Aug6_PresDisp.m’ & run.

MODEL PARAMETERS

1. Find c from toe region in excel (both fiber and perp data together)

0. Add stress/strain data to:

Nov24_costFunction.m

costKappa.m

dispF.m

dispP.m

3. Run Nov24_costFunction.m